全文获取类型
收费全文 | 5060篇 |
免费 | 368篇 |
国内免费 | 3篇 |
出版年
2024年 | 4篇 |
2023年 | 19篇 |
2022年 | 61篇 |
2021年 | 92篇 |
2020年 | 75篇 |
2019年 | 97篇 |
2018年 | 145篇 |
2017年 | 108篇 |
2016年 | 176篇 |
2015年 | 257篇 |
2014年 | 330篇 |
2013年 | 359篇 |
2012年 | 464篇 |
2011年 | 449篇 |
2010年 | 274篇 |
2009年 | 249篇 |
2008年 | 318篇 |
2007年 | 315篇 |
2006年 | 249篇 |
2005年 | 233篇 |
2004年 | 251篇 |
2003年 | 194篇 |
2002年 | 164篇 |
2001年 | 102篇 |
2000年 | 104篇 |
1999年 | 68篇 |
1998年 | 26篇 |
1997年 | 26篇 |
1996年 | 20篇 |
1995年 | 18篇 |
1994年 | 15篇 |
1993年 | 11篇 |
1992年 | 19篇 |
1991年 | 24篇 |
1990年 | 18篇 |
1989年 | 10篇 |
1988年 | 13篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1982年 | 5篇 |
1980年 | 5篇 |
1978年 | 3篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1971年 | 5篇 |
1967年 | 3篇 |
排序方式: 共有5431条查询结果,搜索用时 218 毫秒
861.
Yoon H Blaber SI Evans DM Trim J Juliano MA Scarisbrick IA Blaber M 《Protein science : a publication of the Protein Society》2008,17(11):1998-2007
The human kallikrein-related peptidases (KLKs) comprise 15 members (KLK1-15) and are the single largest family of serine proteases. The KLKs are utilized, or proposed, as clinically important biomarkers and therapeutic targets of interest in cancer and neurodegenerative disease. All KLKs appear to be secreted as inactive pro-forms (pro-KLKs) that are activated extracellularly by specific proteolytic release of their N-terminal pro-peptide. This processing is a key step in the regulation of KLK function. Much recent work has been devoted to elucidating the potential for activation cascades between members of the KLK family, with physiologically relevant KLK regulatory cascades now described in skin desquamation and semen liquefaction. Despite this expanding knowledge of KLK regulation, details regarding the potential for functional intersection of KLKs with other regulatory proteases are essentially unknown. To elucidate such interaction potential, we have characterized the ability of proteases associated with thrombostasis to hydrolyze the pro-peptide sequences of the KLK family using a previously described pro-KLK fusion protein system. A subset of positive hydrolysis results were subsequently quantified with proteolytic assays using intact recombinant pro-KLK proteins. Pro-KLK6 and 14 can be activated by both plasmin and uPA, with plasmin being the best activator of pro-KLK6 identified to date. Pro-KLK11 and 12 can be activated by a broad-spectrum of thrombostasis proteases, with thrombin exhibiting a high degree of selectivity for pro-KLK12. The results show that proteases of the thrombostasis family can efficiently activate specific pro-KLKs, demonstrating the potential for important regulatory interactions between these two major protease families. 相似文献
862.
The Rho guanine nucleotide exchange factor GEF-H1 is uniquely regulated by microtubule binding and is crucial in coupling microtubule dynamics to Rho-GTPase activation in a variety of normal biological situations. Here, we review the roles of GEF-H1 in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. GEF-H1 might also contribute to pathophysiological signaling involved in leukemias, and in cancers associated with mutated p53 tumor suppressor gene, epithelial and endothelial cell dysfunction, infectious disease, and cardiac hypertrophy. We suggest that GEF-H1 could be a novel therapeutic target in multiple human diseases. 相似文献
863.
Boukli L Touaibia M Meddad-Belhabich N Djimdé A Park CH Kim JJ Yoon JH Lamouri A Heymans F 《Bioorganic & medicinal chemistry》2008,16(3):1242-1253
Among the different PLA(2)s identified to date, the group IIA secretory PLA(2) (sPLA(2) GIIA) is implied in diverse pathological conditions. In this work we describe the synthesis, inhibitory activities, and structure-activity relationships (SAR) of a new class of substituted piperazine derivatives. The in vitro fluorimetric assay using two groups of enzymes, GIB and GIIA, revealed several compounds as highly potent inhibitors (IC(50)=0.1 microM). The in vivo activity assessed by ip or per os administration in a carrageenan-induced edema test in rats showed that two compounds proved to be as potent as indomethacin (10 mg/kg). 相似文献
864.
865.
866.
Mind bomb 1-expressing intermediate progenitors generate notch signaling to maintain radial glial cells 总被引:1,自引:0,他引:1
Notch signaling is critical for the stemness of radial glial cells (RGCs) during embryonic neurogenesis. Although Notch-signal-receiving events in RGCs have been well characterized, the signal-sending mechanism by the adjacent cells is poorly understood. Here, we report that conditional inactivation of mind bomb-1 (mib1), an essential component for Notch ligand endocytosis, in mice using the nestin and hGFAP promoters resulted in complete loss of Notch activation, which leads to depletion of RGCs, and premature differentiation into intermediate progenitors (IPs) and finally neurons, which were reverted by the introduction of active Notch1. Interestingly, Mib1 expression is restricted in the migrating IPs and newborn neurons, but not in RGCs. Moreover, sorted Mib1+ IPs and neurons can send the Notch signal to neighboring cells. Our results reveal that not only newborn neurons but also IPs are essential Notch-ligand-presenting cells for maintaining RGC stemness during both symmetric and asymmetric divisions. 相似文献
867.
Toll-like receptor 9-stimulated monocyte chemoattractant protein-1 is mediated via JNK-cytosolic phospholipase A2-ROS signaling 总被引:1,自引:0,他引:1
Lee JG Lee SH Park DW Lee SH Yoon HS Chin BR Kim JH Kim JR Baek SH 《Cellular signalling》2008,20(1):105-111
Monocyte chemoattractant protein-1 (MCP-1) influences monocyte migration into sites of inflammation. This study highlights the importance of cytosolic phospholipase A2 (cPLA2)-mediated reactive oxygen species (ROS) signaling processes in the regulation of MCP-1 release as a result of toll-like receptor (TLR) activation. In macrophages, activation of TLR9 induced MCP-1 and cPLA2-phosphorylated arachidonic acid (AA) release. Inhibition of cPLA2 blocked CpG-induced MCP-1 and AA release. Although CpG stimulates phosphorylation of ERK, p38 and JNK, only inhibition of the JNK signaling pathways attenuated MCP-1 release, suggesting that the TLR9-mediated MCP-1 release was dependent upon the JNK pathway. TLR9 activation also stimulated ROS generation, while inhibition of NADPH oxidases (Noxs) blocked CpG-induced MCP-1 release. The CpG treatment increased macrophage Nox1 mRNA level, however it had no effect on macrophage Nox2 mRNA level. Overall, these results suggest that CpG enhances ROS generation through cPLA2-dependent pathways, which results in MCP-1 release. 相似文献
868.
Mi Yoon Chung 《Ecological Research》2008,23(1):83-90
Fine-scale genetic structure (FSGS) in plant populations is expected to be influenced by variation in demographic processes
across space and over time. I chose Hemerocallis taeanensis (Liliaceae), a perennial herb with a rapid population turnover, to quantify how demographic structure and FSGS change with
a population’s history (i.e., density). Nonaccumulative O-ring statistic and spatial autocorrelation analysis (kinship coefficient,
F
ij
) were used to quantify spatial patterns of individuals and FSGS in four populations belonging to two population stages (expansion
and maturation) in west-central Korea. The O-ring function revealed that significant aggregation of individuals occurs at
short spatial scales during the earlier stage of population expansion, which reflects restricted seed dispersal around maternal
individuals. However, this pattern disappears as the population density increases during population maturation, probably due
to a high population density. Significant evidence of FSGS was found in two populations at the stage of population expansion
(Sp, a statistic which describes the rate of decrease of pairwise kinship with distance, was 0.018 and 0.029). The results show
that most seeds fall around maternal plants when initially established colonists proliferate at suitable microhabitats. In
contrast to this, much lower Sp values (−0.003 and 0.004) were estimated for two populations at the stage of population maturation, which may result from
the overlapping of seed shadows due to high adult density. All of these results demonstrate considerable variation in within-population
demographic and genetic structures of H. taeanensis with respect to population temporal stage across the landscape. 相似文献
869.
The enzymes phosphoglucomutase (PGM) and phosphomannomutase (PMM) play an important role in the synthesis of extracellular polysaccharide. By colony hybridization of the fosmid library of Sphingomonas chungbukensis DJ77, an open reading frame (ORF-1) of 1,626 nucleotides, whose predicted product is highly homologous with other PGM proteins from several bacterial species, was identified. An additional open reading frame (ORF-2) of 1,437 nucleotides was identified, and its encoded protein shows a high level of similarity with the PGM/PMM protein family. The two genes were cloned into a bacterial expression vector pET-15b (+) and expressed in Escherichia coli as fusion proteins with (His)(6)-tag. Both recombinant proteins (designated as SP-1 and SP-2 for ORF-1 and ORF-2, respectively) exhibited PGM and PMM activities. The molecular masses of subunits of SP-1 and SP-2 were estimated to be around 58 and 51 kDa from SDS-PAGE, respectively. However, molecular masses of SP-1 and SP-2 in their native condition were determined to be approximately 59.5 and 105.4 kDa, according to non-denaturing PAGE, respectively. The SP-1 protein has a preference for glucose-1-phosphate rather than mannose-1-phosphate, while the preferred substrate of SP-2 is mannose-1-phosphate. Thus, the existence of two proteins with bifunctional PGM/PMM activities was first found S. chungbukensis DJ77. 相似文献
870.
Lee HJ Yoon YJ Jang do S Kim C Cha HJ Hong BH Choi KY Lee HC 《Journal of biochemistry》2008,144(2):159-166
The backbone dynamics of Y14F mutant of Delta(5)-3-ketosteroid isomerase (KSI) from Comamonas testosteroni has been studied in free enzyme and its complex with a steroid analogue, 19-nortestosterone hemisuccinate (19-NTHS), by (15)N NMR relaxation measurements. Model-free analysis of the relaxation data showed that the single-point mutation induced a substantial decrease in the order parameters (S(2)) in free Y14F KSI, indicating that the backbone structures of Y14F KSI became significantly mobile by mutation, while the chemical shift analysis indicated that the structural perturbations of Y14F KSI were more profound than those of wild-type (WT) KSI upon 19-NTHS binding. In the 19-NTHS complexed Y14F KSI, however, the key active site residues including Tyr14, Asp38 and Asp99 or the regions around them remained flexible with significantly reduced S(2) values, whereas the S(2) values for many of the residues in Y14F KSI became even greater than those of WT KSI upon 19-NTHS binding. The results thus suggest that the hydrogen bond network in the active site might be disrupted by the Y14F mutation, resulting in a loss of the direct interactions between the catalytic residues and 19-NTHS. 相似文献