全文获取类型
收费全文 | 47537篇 |
免费 | 4846篇 |
国内免费 | 1796篇 |
专业分类
54179篇 |
出版年
2024年 | 68篇 |
2023年 | 386篇 |
2022年 | 937篇 |
2021年 | 1606篇 |
2020年 | 1125篇 |
2019年 | 1387篇 |
2018年 | 1463篇 |
2017年 | 1097篇 |
2016年 | 1634篇 |
2015年 | 2382篇 |
2014年 | 2701篇 |
2013年 | 2951篇 |
2012年 | 3351篇 |
2011年 | 3156篇 |
2010年 | 1946篇 |
2009年 | 1739篇 |
2008年 | 2136篇 |
2007年 | 1950篇 |
2006年 | 1762篇 |
2005年 | 1504篇 |
2004年 | 1277篇 |
2003年 | 1179篇 |
2002年 | 991篇 |
2001年 | 2675篇 |
2000年 | 2416篇 |
1999年 | 1854篇 |
1998年 | 655篇 |
1997年 | 705篇 |
1996年 | 625篇 |
1995年 | 553篇 |
1994年 | 473篇 |
1993年 | 369篇 |
1992年 | 910篇 |
1991年 | 756篇 |
1990年 | 614篇 |
1989年 | 488篇 |
1988年 | 378篇 |
1987年 | 340篇 |
1986年 | 254篇 |
1985年 | 202篇 |
1984年 | 135篇 |
1983年 | 101篇 |
1982年 | 80篇 |
1981年 | 67篇 |
1980年 | 48篇 |
1979年 | 55篇 |
1978年 | 44篇 |
1976年 | 41篇 |
1974年 | 42篇 |
1972年 | 40篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
The selenoprotein A component of the glycine reductase complex of Clostridium sticklandii was shown to differ in certain properties from the selenoprotein A produced by a purine-fermenting organism, Clostridium purinolyticum. Both proteins contain one selenocysteine and two cysteine residues. 相似文献
102.
Ferricrocin functions as the main intracellular iron-storage compound in mycelia ofNeurospora crassa
Summary
Neurospora crassa produces several structurally distinct siderophores: coprogen, ferricrocin, ferrichrome C and some minor unknown compounds. Under conditions of iron starvation, desferricoprogen is the major extracellular siderophore whereas desferriferricrocin and desferriferrichrome C are predominantly found intracellularly. Mössbauer spectroscopic analyses revealed that coprogen-bound iron is rapidly released after uptake in mycelia of the wild-typeN.crassa 74A. The major intracellular target of iron distribution is desferriferricrocin. No ferritin-like iron pools could be detected. Ferricrocin functions as the main intracellular iron-storage peptide in mycelia ofN. crassa. After uptake of ferricrocin in both the wild-typeN. crassa 74A and the siderophore-free mutantN. crassa arg-5 ota aga, surprisingly little metabolization (11%) could be observed. Since ferricrocin is the main iron-storage compound in spores ofN. crassa, we suggest that ferricrocin is stored in mycelia for inclusion into conidiospores. 相似文献
103.
104.
X M Zhang E M McDowell 《Virchows Archiv. B, Cell pathology including molecular pathology》1992,61(6):375-387
We showed previously that the proliferation of hamster airway secretory cells decreases during vitamin A deficiency (VAD) but later increases when submucosal inflammation develops (Virchows Arch [B] 59:231-242, 1990). This observation has important biological implications since two morphological extremes (atrophy and quiescence versus hyperplasia and hyperproliferation) are reported in the literature for VAD tracheal epithelium in vivo. In the present study, histological slides of tracheal rings from 35-day-old control and VAD hamsters (Virchows Arch [B] 45:197-219, 1984) were reviewed again. Rings from VAD hamsters were selected based on the absence or presence of a florid submucosal inflammation. Quantitative analyses were made on the cartilaginous part of rings from the anterior third of the trachea. When inflammation was absent, a mucociliary pseudostratified epithelium was, for the most part, maintained. The mitotic rate (MR, 6 h colchicine blockade) of secretory cells was markedly reduced (29-fold) but that of basal cells was not changed significantly. Moreover, cell density was not changed by VAD but ciliated cells and secretory cells were decreased and basal cells were increased, proportionally. We call this "minimal morphological change." Thinning (atrophy) of the minimally changed epithelium was associated with focal cell sloughing. Small scattered foci of epidermoid metaplasia (multiple layers of highly keratinized cells which were extremely flat, so that the epithelium was thin and attenuated) were also seen. We call this "atrophic epidermoid metaplasia." When inflammation was present, hyperplastic changes (stratification and epidermoid metaplasia) predominated and cells were in mitosis at all epithelial levels (low, middle, superficial) except in the most superficial (terminally differentiated) squames. The tracheal epithelium was thickened and hypercellular. The cells were piled up at the stratified lesions, and epithelial height, cell density and epithelial MR were significantly increased compared with the non-inflamed VAD epithelium. The effects of VAD and inflammation on cell proliferation were analyzed further by studying 7 h bromodeoxyuridine (BrdU) labelling patterns of cells in VAD tracheal epithelium, with and without submucosal inflammation. In addition, inflammation was induced in "minimally changed epithelium" by mild mechanical injury. The BrdU labelling patterns confirmed that DNA synthesis by secretory cells is reduced markedly by VAD. However, this suppression is overidden by the influx of inflammatory cells (the nature of the stimulus is unknown). The results indicate that the morphological contrasts (atrophy and hyperplasia) seen in the trachea during VAD in vivo are related to extremes in proliferation rates of tracheal secretory cells, regulated by VAD alone (minimal replication) and by inflammation (maximal replication). 相似文献
105.
头端延髓腹外侧区注射5—羟色胺对应激性高血粘度... 总被引:3,自引:0,他引:3
Experiments were carried out on 62 wistar rats. The hyperviscosity and elevation of blood pressure were induced by hanging and restraining the rats with their four limbs tied on a frame. It was found that microinjection of 5-HT (25 micrograms/10 microliters) into the 4th ventricle of the brain or bilateral microinjection of 5-HT (4 micrograms/0.5 microliters/site) into rostral ventrolateral medulla (rVLM) reduced stress-induced hyperviscosity (p < 0.01) and elevation of blood pressure (p < 0.01). The effect of 5-HT injected into the 4th ventricle or rVLM was blocked by bilateral microinjection of cinanserine (4 micrograms/0.5 microliter/site) into rVLM. These results suggest that microinjection of 5-HT into 4th ventricle and rVLM could reduce stress-induced hyperviscosity and elevation of blood pressure and these effects were probably mediated via 5-HT receptors in the rVLM. 相似文献
106.
107.
108.
X Tang H Tashiro T Eki Y Murakami E Soeda T Sakakura P C Watkins K Yokoyama 《Genomics》1992,14(1):185-187
Sequence-tagged sites (STSs) are short stretches of DNA that can be specifically detected by the polymerase chain reaction (PCR) and can be used to construct long-range physical maps of chromosomal DNA. These STSs can be detected by PCR assays developed by reference to data obtained from the sequencing of restriction fragment length polymorphism-DNA markers for chromosome 21, which were derived from recombinant lamba-phage and plasmid clones made from DNA of a human-hamster hybrid cell line. In this report, we describe the generation of 19 new STSs that are specific for human chromosome 21. 相似文献
109.
Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5. 总被引:14,自引:0,他引:14
D Sieburth E W Jabs J A Warrington X Li J Lasota S LaForgia K Kelleher K Huebner J J Wasmuth S F Wolf 《Genomics》1992,14(1):59-62
IL12 (formerly NKSF or CLMF) is a unique cytokine composed of two unrelated disulfide-linked subunits. The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF. The chromosomal localization of these two subunits was determined by PCR analysis of DNA from rodent-human hybrids. More refined mapping was obtained by PCR analysis of hybrids containing translocation chromosomes and for p40, by analysis of radiation hybrids. The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2. 相似文献
110.
Human dopamine transporter gene (DAT1) maps to chromosome 5p15.3 and displays a VNTR. 总被引:24,自引:0,他引:24
D J Vandenbergh A M Persico A L Hawkins C A Griffin X Li E W Jabs G R Uhl 《Genomics》1992,14(4):1104-1106
The human dopamine transporter (DAT1) gene is localized to chromosome 5p15.3 by in situ hybridization and PCR amplification of rodent somatic cell hybrid DNA. Analysis of a 40-bp repeat in the 3' untranslated region of the message revealed variable numbers of the repeat ranging from 3 to 11 copies. These results will aid in the investigation of a role for this gene in genetic disorders of the dopaminergic system in humans. 相似文献