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151.
广东鼎湖山自然保护区的亚热带季风常绿阔叶林,是我国季风亚热带具典型地带性植被类型。目前,除了保持它的原始林外,还有部分植被因人为活动而受破坏,代之以次生林。原始林内植被的覆盖造成的微环境是一个趋于平衡的生态系统。植物类型与植被环境的变化,改变了植物与昆虫已建立的固定关系,使昆虫栖息环境、食物数量和适食性都发生了变化。对两种林地植物受虫害的研究,有助于阐明植被改变后的生态后果。 相似文献
152.
Hong Zhao Lijie Yuan Dongli Zhu Banghao Sun Juan Du Jingyuan Wang 《Polish journal of microbiology》2022,71(2):173
To explore the role of gut microbiota in Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). Seventy fecal samples were collected, including 27 patients with GD, 27 with HT, and 16 samples from healthy volunteers. Chemiluminescence was used to detect thyroid function and autoantibodies (FT3, FT4, TSH, TRAb, TGAb, and TPOAb); thyroid ultrasound and 16S sequencing were used to analyze the bacteria in fecal samples; KEGG (Kyoto Encyclopedia of Genes and Genomes) and COG (Clusters of Orthologous Groups) were used to analyze the functional prediction and pathogenesis. The overall structure of gut microbiota in the GD and HT groups was significantly different from the healthy control group. Proteobacteria and Actinobacteria contents were the highest in the HT group. Compared to the control group, the GD and HT groups had a higher abundance of Erysipelotrichia, Cyanobacteria, and Ruminococcus_2 and lower levels of Bacillaceae and Megamonas. Further analysis of KEGG found that the “ABC transporter” metabolic pathway was highly correlated with the occurrence of GD and HT. COG analysis showed that the GD and HT groups were enriched in carbohydrate transport and metabolism compared to the healthy control group but not in amino acid transport and metabolism. Our data suggested that Bacillus, Blautia, and Ornithinimicrobium could be used as potential markers to distinguish GD and HT from the healthy population and that “ABC transporter” metabolic pathway may be involved in the pathogenesis of GD and HT. 相似文献
153.
Erythropoietin is a major regulator of erythropoiesis which maintains the body's red blood cell mass and tissue oxygenation at an optimum level. Recombinant human erythropoietin (rhEPO), which is a widely used therapeutic agent for the treatment of anemia and which represents one of the largest biopharmaceuticals markets, is produced from recombinant Chinese hamster ovary cells. rhEPO is a glycoprotein with complex glycan structure, which is responsible for its therapeutic efficacy, including the in vivo activity and half-life. In order to obtain an optimal and consistent glycoform profile of rhEPO and concurrently maintain a high production yield, various approaches in drug development and cell culture technology have been attempted. Recent advances in rhEPO production are classified into three types: the development of improved rhEPO molecules by protein engineering; improvement of production host cells by genetic engineering; and culture condition optimization by fine control of the production mode/system, process parameters, and culture media. In this review, we focus on rhEPO production strategies as they have progressed thus far. Furthermore, the current status of the market and outlook on rhEPO and its derivatives are discussed. 相似文献
154.
Enhancement of hyperthermia-induced apoptosis by local anesthetics on human histiocytic lymphoma U937 cells 总被引:2,自引:0,他引:2
Arai Y Kondo T Tanabe K Zhao QL Li FJ Ogawa R Li M Kasuya M 《The Journal of biological chemistry》2002,277(21):18986-18993
The combined effects of hyperthermia at 44 degrees C and local anesthetics on apoptosis in human histiocytic lymphoma U937 cells were investigated. When the cells were exposed to hyperthermia for l0 min marginal DNA fragmentation and nuclear fragmentation were observed. In the presence of amide-type local anesthetics further enhancement was found depending on concentration. The order of the concentration required for maximum induction was the reverse order of the lipophilicity (prilocaine > lidocaine > bupivacaine). Western blotting revealed that in hyperthermia there was initial release of Ca(2+) from the intracellular store site as indicated by increased expression of the type 1 inositol-1,4,5-trisphosphate receptor. However, the combination with lidocaine did not induce any further enhancement. Lidocaine enhanced the decrease in ATP content and the increase in intracellular Ca(2+) concentration in individual cells induced by hyperthermia. In addition, superoxide formation, decrease in the mitochondrial membrane potential, and activation of intracellular caspase-3 were found in the cells treated with hyperthermia and lidocaine. All of these were suppressed in part in the presence of the intracellular Ca(2+) ion chelator BAPTA-AM (bis-(O-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl). The present results indicate that local anesthetics at optimal concentrations enhance hyperthermia-induced apoptosis via Ca(2+)- and mitochondria-dependent pathways. Initial release of Ca(2+) from intracellular store sites caused by hyperthermia and followed by the subsequent increase in the intracellular Ca(2+) concentration and the additional activation of the mitochondrial caspase-dependent pathway (partly regulated by intracellular Ca(2+) concentration) plays a crucial role in the enhancement of apoptosis induced by the combination of hyperthermia and lidocaine. 相似文献
155.
摘要:【目的】结合纳米技术建立检测大肠杆菌(Escherichia coli)O157︰H7高灵敏检测技术。【方法】采用化学共沉淀法制备出核心粒径约为10 nm的免疫纳米磁颗粒,柠檬酸钠还原法制备粒径约为20 nm的免疫胶体金。压电免疫传感器通过金黄色葡萄球菌蛋白A(Protein A from Staphylococcus aureus SPA)法将抗体固定于石英晶振上,两种免疫纳米颗粒借助不同的抗体连接于传感器上对检测频率信号进行放大。【结果】SPA在石英晶振上的最佳固定浓度和时间为1.2 mg/mL和40 min,抗体的最佳固定浓度和时间为1.0 mg/mL和60 min。压电免疫传感器通过两种免疫纳米颗粒的放大作用,使其对大肠杆菌O157︰H7的检测限从104 cfu/mL提高到101 cfu/mL。【结论】免疫纳米颗粒强化对压电免疫传感器的检测频率信号具有很好的放大效应,可以明显提高其检测灵敏度。 相似文献
156.
以聚丙烯酰胺凝胶电泳方法测定了331例辽宁满族新生儿脐带血血红蛋白F中Gγ/Aγ比值。结果显示:高Gγ(>80%)者4例,占1.21%,其基因频率(f)为0.00604,低Gγ(30-48%)者6例,占1.81%,其基因频率为0.00906,其余正常,Gγ平均值为65.23±6.38%。未发现AγT链。对其中八例异常者(4例高Gγ值,4例低Gγ值)的染色体DNA进行了基因图谱分析,确定4例高Gγ值者基因型有两种:Gγ-Gγ/Gγ-Aγ二例,Gγ-AGγ-Aγ/Gγ-Aγ二例;4例低Gγ值者基因型有二种,分别为:Aγ-Aγ/Gγ-Aγ二例和-GAγ/Gγ-Aγ二例。其中Aγ-Aγ基因型在中国人群中未见报道过。 相似文献
157.
158.
159.
溶栓剂DSPAα1正处于治疗急性缺血性中风的III期临床研究,临床结果显示DSPAα1具有良好的药理学和安全特性。将DSPAα1基因序列按照毕赤酵母偏好密码子进行优化,并在毕赤酵母菌株GS115和KM71中进行表达,同时利用定点突变对糖基化侧链进行缺失,考察糖基侧链对毕赤酵母表达DSPAα1的影响。结果表明,野生型DSPAα1在GS115和KM71中均获得高表达,在摇瓶发酵条件下,表达量分别为70mg/L和105mg/L;利用SDS-PAGE对DSPAα1三种突变体(N117Q、N362Q和N117Q/N362Q)进行分析,与野生型蛋白质相比较,3种突变体的表达水平显著下降,同时纤溶平板测活数据显示,纯化后的突变体N117Q和N362Q比活性均低于野生型蛋白质的25%。这表明,N-型糖链(N117和N362)对毕赤酵母表达的DSPAα1分泌和酶活性具有重要作用。 相似文献
160.