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911.
A new lectin gene was cloned from Amorphophallus konjac. The full-length cDNA of Amorphophallus konjac agglutinin (aka) was 736 bp and contained a 474 bp open reading frame encoding a 158 amino acid protein. Homology analysis revealed that the lectin from this Araceae species belonged to the superfamily of monocot mannose-binding proteins. Molecular modeling of AKA indicated that the three-dimensional structure of AKA strongly resembles that of the snowdrop lectin. Southern blot analysis of the genomic DNA revealed that aka belonged to a low-copy gene family. Northern blot analysis demonstrated that aka expression was tissue-specific with the strongest expression being found in root. 相似文献
912.
Differential effects of two growth inhibitory K vitamin analogs on cell cycle regulating proteins in human hepatoma cells 总被引:6,自引:0,他引:6
Markovits J Wang Z Carr BI Sun TP Mintz P Le Bret M Wu CW Wu FY 《Life sciences》2003,72(24):2769-2784
A comparison was made between two K vitamin analogs. Growth in vitro of Hep G2 hepatoma cells was inhibited both by Compound 5 (Cpd 5), a recently synthesized thioalkyl analog of vitamin K or 2-(2-mercaptoethanol)-3-methyl-1, 4-naphthoquinone, as well as by synthetic vitamin K3 (menadione). Using synchronized Hep G2 hepatoma cells, the actions of both Cpd 5 and vitamin K3 on cell cycle regulating proteins were examined. Cpd 5 decreased the levels of cyclin D1, Cdk4, p16, p21 and cyclin B1. By contrast, VK3 only decreased the level of cyclin D1, but had no effect on the levels of Cdk4, p16 or p21. Interestingly, both VK3 and VK2 increased the levels of p21. The naturally occurring K vitamins had little effect on cell growth and none on the cyclins or Cdks. Amounts and activity of the G1/S phase controlling Cdc25A were measured. We found that Cpd 5 directly inhibited both Cdc25A activity and its protein expression, whereas VK3 did not. Thus, the main effects of Cpd 5 were on G1 and S phase proteins, especially Cdk4 and Cdc25A amounts in contrast to VK3. Computer docking studies of Cpd 5 and VK3 to Cdc25A phosphatase showed three binding sites. In the best conformation, Cpd 5 was found to be closer to the enzyme active site than VK3. These findings show that Cpd 5 represents a new class of anticancer agent, being a protein tyrosine phosphatase (PTP) antagonist, that binds to Cdc25A with suppression of its activity. Tumors expressing high levels of oncogenic Cdc25A phosphatase may thus be susceptible to the growth inhibitory activities of this class of compound. 相似文献
913.
914.
915.
Adipogenic differentiating agents regulate expression of fatty acid binding protein and CD36 in the J744 macrophage cell line 总被引:2,自引:0,他引:2
Adipocyte fatty acid binding protein (aP2) is a key mediator of intracellular transport and metabolism of fatty acids. Its expression during adipocyte differentiation is regulated through the actions of peroxisome proliferator-activated receptor gamma (PPARgamma) and CCAAT/enhancer binding protein alpha (C/EBPalpha). Macrophages also express aP2, and the lack of macrophage aP2 significantly reduces atherosclerotic lesion size in hypercholesterolemic mice. We investigated the regulation of expression of macrophage aP2 and CD36, a fatty acid membrane binding protein and scavenger receptor, in response to the adipogenic agents isobutylmethylxanthine (IBMX), insulin, and dexamethasone, a combination of agents shown to induce fibroblast-to-adipocyte differentiation. Treatment of J774 macrophages with adipogenic agents significantly induced aP2 mRNA expression, while CD36 expression was inhibited. Dexamethasone was essential and sufficient to induce aP2 expression, and insulin had a synergistic effect. However, IBMX antagonized induced-aP2 expression. aP2 protein expression and [14C]oleic acid uptake by macrophages were also increased by dexamethasone. Unlike what occurs in adipocytes, adipogenic agents had mixed effects on the expression of PPARgamma and C/EBPalpha in macrophages. Our data demonstrate differences in the regulation of aP2 in adipocytes and macrophages and show that macrophage aP2 expression by adipogenic agents is independent of the PPARgamma and/or C/EBPalpha signaling pathway. 相似文献
916.
利用离体诱变技术选育小麦大粒种质的研究 总被引:3,自引:0,他引:3
以鲁麦12等为材料进行幼层培养,转分化前辐照1000rad,在R2代调查千粒重变异情况,结果表明,后代千粒重发生了显著变化,变异范围为39.5—68.5g.突变率高达63.08%,最高千粒重超过对照40.95%。已选出一批大粒材料应用于生产.其中核生二号新品种千粒重65g,杂交后代表明,大粒变异多为显性突变。 相似文献
917.
Cloning and functional characterization of LCR-F1: a bZIP transcription factor that activates erythroid-specific,human globin gene expression. 下载免费PDF全文
DNase I hypersensitive site 2 (HS 2) of the human beta-globin Locus Control Region (LCR) directs high level expression of the beta-globin gene located 50 kilobases downstream. Experiments in cultured cells and in transgenic mice demonstrate that duplicated AP1-like sites in HS 2 are required for this powerful enhancer activity. A cDNA clone encoding a basic, leucine-zipper protein that binds to these sites was isolated and designated Locus Control Region-Factor 1 (LCR-F1). This protein is a member of a new family of regulatory factors that contain a 63 amino acid ''CNC domain'' overlapping the basic region. This domain is approximately 70% identical in the Drosophila Cap N Collar (CNC) protein, NF-E2 and LCR-F1. LCR-F1 transactivates an HS 2/gamma-globin reporter gene over 170-fold in transient transfection experiments specifically in erythroid cells. These results suggest that LCR-F1 may be a critical factor involved in LCR-mediated, human globin gene expression. 相似文献
918.
白额鹱卵壳的扫描电镜观察 总被引:9,自引:2,他引:9
本文报道白额鹱卵壳的壳膜、孔锥层、海绵层、表层等的超微结构,并对卵壳元素进行TN-5500能谱分析。 相似文献
919.
皂荚皂甙提取工艺的研究 总被引:7,自引:0,他引:7
研究了以皂荚为原料,对传统有机溶剂提取与水提取和超临界CO2萃取三萜皂甙进行了比较,得出水最适合提取皂荚皂甙;水提工艺部分,在单因素实验的基础上采用正交设计实验优化工艺,得出提取皂荚皂甙的最佳工艺。 相似文献
920.
松嫩平原盐碱化草地治理方法的比较研究 总被引:22,自引:0,他引:22
采用生物、化学和物理方法对盐碱化草地治理进行了比较研究.生物方法采用枯草处理;化学方法采用石膏处理;物理方法采用铺沙处理,种植羊草、野大麦和碱茅3种牧草.结果表明,3种方法均能降低土壤pH值、电导率和增加土壤含水率.在生物治理中,羊草和野大麦在枯草量达1500g·m-2,碱茅在枯草量达1000g·m-2时,便可良好生长.在化学治理中,石膏施用量达1.25kg·m-2,即可达到改良效果.在物理方法治理中,羊草和野大麦在沙层厚度为13cm,碱茅在11cm时即可正常生长发育. 相似文献