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201.
Jingliang Cheng Jiangzhou Peng Jiewen Fu Md. Asaduzzaman Khan Pingping Tan Chunli Wei Xiyun Deng Hanchun Chen Junjiang Fu 《Journal of cellular and molecular medicine》2020,24(2):1676-1683
Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer‐related deaths in women worldwide. In this study, a large Chinese pedigree with breast cancer including a proband and two female patients was recruited and a familial history of breast cancer was collected by questionnaire. Clinicopathological assessments and neoadjuvant therapy‐related information were obtained for the proband. Blood samples were taken, and gDNA was extracted. The BRCA1/2 and PALB2 genes were screened using next‐generation sequencing by a targeted gene panel. We have successfully identified a novel, germline heterozygous, missense mutation of the gene BRCA2: c.7007G>T, p.R2336L, which is likely to be pathogenic in the proband and her elder sister who both had breast cancer. Furthermore, the risk factors for developing breast cancer in this family are discussed. Thus, genetic counselling and long‐term follow‐up should be provided for this family of breast cancer patients as well as carriers carrying a germline variant of BRCA2: c.7007G>T (p.R2336L). 相似文献
202.
Regmi Sudip Choi Yoon Seok Kim Young Kyun Khan Md Maruf Lee Sang Hun Choi Yun Hee Cho Seung Sik Jin Ying-Yu Yoo Jin Cheol Suh Joo-Won 《Bioprocess and biosystems engineering》2020,43(2):249-259
Bioprocess and Biosystems Engineering - The β-glucanase produced from Bacillus sp. CSB55 not only depicts the potent industrial characteristics but also relates as bio-industrial catalyst... 相似文献
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205.
Shazmeen Aslam Irfan Khan Fatima Jameel Midhat Batool Zaidi Asmat Salim 《World journal of stem cells》2020,12(12):1652-1666
BACKGROUNDImpaired wound healing can be associated with different pathological states. Burn wounds are the most common and detrimental injuries and remain a major health issue worldwide. Mesenchymal stem cells (MSCs) possess the ability to regenerate tissues by secreting factors involved in promoting cell migration, proliferation and differentiation, while suppressing immune reactions. Preconditioning of MSCs with small molecules having cytoprotective properties can enhance the potential of these cells for their use in cell-based therapeutics.AIMTo enhance the therapeutic potential of MSCs by preconditioning them with isorhamnetin for second degree burn wounds in rats.METHODSHuman umbilical cord MSCs (hU-MSCs) were isolated and characterized by surface markers, CD105, vimentin and CD90. For preconditioning, hU-MSCs were treated with isorhamnetin after selection of the optimized concentration (5 µmol/L) by cytotoxicity analysis. The migration potential of these MSCs was analyzed by the in vitro scratch assay. The healing potential of normal, and preconditioned hU-MSCs was compared by transplanting these MSCs in a rat model of a second degree burn wound. Normal, and preconditioned MSCs (IH + MSCs) were transplanted after 72 h of burn injury and observed for 2 wk. Histological and gene expression analyses were performed on day 7 and 14 after cell transplantation to determine complete wound healing.RESULTSThe scratch assay analysis showed a significant reduction in the scratch area in the case of IH + MSCs compared to the normal untreated MSCs at 24 h, while complete closure of the scratch area was observed at 48 h. Histological analysis showed reduced inflammation, completely remodeled epidermis and dermis without scar formation and regeneration of hair follicles in the group that received IH + MSCs. Gene expression analysis was time dependent and more pronounced in the case of IH + MSCs. Interleukin (IL)-1β, IL-6 and Bcl-2 associated X genes showed significant downregulation, while transforming growth factor β, vascular endothelial growth factor, Bcl-2 and matrix metallopeptidase 9 showed significant upregulation compared to the burn wound, showing increased angiogenesis and reduced inflammation and apoptosis.CONCLUSIONPreconditioning of hU-MSCs with isorhamnetin decreases wound progression by reducing inflammation, and improving tissue architecture and wound healing. The study outcome is expected to lead to an improved cell-based therapeutic approach for burn wounds. 相似文献
206.
Afrah F. Alkhuriji Seham G. Alsaiari Suliman Y. Alomar Alaa A. Alnafjan Hussah Alobaid Manal F. El-Khadragy 《Bioscience reports》2021,41(3)
Colon cancer is one of the most common causes of deaths by cancer worldwide. Stem cells have immunosuppressive properties that promote tumor targeting and circumvent obstacles currently in gene therapy. Bone marrow stem cells are believed to have anticancer potential. The transplantation of mesenchymal stem cells (MSCs), a type of bone marrow stem cells, has been considered a potential therapy for patients with solid tumors due to their capability to enhance the immune response; MSC transplantation has received renewed interest in recent years. The present study aimed to evaluate the antiapoptotic effects of the MSCs on 1,2-dimethylhydrazine (DMH)-induced inflammation in the rat model of colorectal cancer. The rats were randomly allocated into four groups: control, treated with MSCs, induced by DMH, and induced by DMH and treated with MSCs. The MSCs were intra-rectally injected, and DMH was subcutaneously injected at 20 mg/kg body weight once a week for 15 weeks. The administration of MSCs into rats starting from day 0 of the DMH injection was found to enhance the histopathological picture. The MSC treatment resulted in fewer inflammatory cells than in the DMH group. Therefore, our findings suggest that BMCs have antitumor effects by modulating the cellular redox status and down-regulating the pro-inflammatory genes. Thus, BMCs may provide therapeutic value for colon cancer treatment. 相似文献
207.
Barkat Ali Wasim Sajjad Prakriti Sharma Ghimire Suliman Khan Ghufranud Din 《Geomicrobiology journal》2021,38(1):1-13
Abstract High-throughput sequencing approach of the 16S rRNA gene was employed to evaluate the bacterial diversity inhabit in melted water, snow, soil, and rocks samples at the lower altitudes of the Laohugou glacial environment. Bioinformatics tools were used to process millions of Illumina reads for alpha and beta diversities of bacterial communities. The diversity indices such as Chao, Shannon, and Simpson were different in the collected samples and solid samples (soil and rocks) showed higher taxon richness and evenness. Taxonomic diversity was unexpectedly higher and the major portion of sequences was assigned to Proteobacteria, Actinobacteria, and Acidobacteria. Higher variation in community structure was reported at the class level and Alphaproteobacteria was dominant. The solid niches were occupied by a higher number of phyla compared with liquid. The physicochemical variables acted as spatial gradients and associated with the bacterial structural communities of the glacial ecosystem. Findings showed that both Proteobacteria and Actinobacteria in solid samples influenced the bacterial community structure in downstream liquid samples. Interestingly, the metagenomic biomarkers were higher in liquid samples. This study provides precious datasets to understand the bacterial community in a better way under the influence of spatial, physical and environmental factors. 相似文献
208.
Molybdenum supply increases root system growth of winter wheat by enhancing nitric oxide accumulation and expression of NRT genes 总被引:1,自引:0,他引:1
Imran Muhammad Sun Xuecheng Hussain Saddam Rana Muhammad Shoaib Saleem Muhammad Hamzah Riaz Muhammad Tang Xiangru Khan Imran Hu Chengxiao 《Plant and Soil》2021,462(1-2):235-244
Plant and Soil - Success in agronomic biofortification of maize and wheat is highly variable. This study aimed to elucidate the differences in uptake and translocation of foliar-applied zinc (Zn)... 相似文献
209.
Wagatsuma Tadao Tanaka Kazuhiro Iino Yuuichirou Satou Tsuyoshi Khan Md. Shahadat Hossain Tawaraya Keitaro 《Plant and Soil》2021,459(1-2):289-313
Plant and Soil - The major factors controlling the soil methane (CH4) concentration and CH4 emissions of various plant (mainly wetland) species were identified. Five plant species (Oryza sativa,... 相似文献
210.