Effect of mesenchymal stem cells on cytochrome-c release and inflammation in colon cancer induced by 1,2-dimethylhydrazine in Wistar albino rats

Colon cancer is one of the most common causes of deaths by cancer worldwide. Stem cells have immunosuppressive properties that promote tumor targeting and circumvent obstacles currently in gene therapy. Bone marrow stem cells are believed to have anticancer potential. The transplantation of mesenchymal stem cells (MSCs), a type of bone marrow stem cells, has been considered a potential therapy for patients with solid tumors due to their capability to enhance the immune response; MSC transplantation has received renewed interest in recent years. The present study aimed to evaluate the antiapoptotic effects of the MSCs on 1,2-dimethylhydrazine (DMH)-induced inflammation in the rat model of colorectal cancer. The rats were randomly allocated into four groups: control, treated with MSCs, induced by DMH, and induced by DMH and treated with MSCs. The MSCs were intra-rectally injected, and DMH was subcutaneously injected at 20 mg/kg body weight once a week for 15 weeks. The administration of MSCs into rats starting from day 0 of the DMH injection was found to enhance the histopathological picture. The MSC treatment resulted in fewer inflammatory cells than in the DMH group. Therefore, our findings suggest that BMCs have antitumor effects by modulating the cellular redox status and down-regulating the pro-inflammatory genes. Thus, BMCs may provide therapeutic value for colon cancer treatment.     

Culture Independent Diversity of Bacterial Communities Indigenous to Lower Altitude at Laohugou Glacial Environment

Abstract

High-throughput sequencing approach of the 16S rRNA gene was employed to evaluate the bacterial diversity inhabit in melted water, snow, soil, and rocks samples at the lower altitudes of the Laohugou glacial environment. Bioinformatics tools were used to process millions of Illumina reads for alpha and beta diversities of bacterial communities. The diversity indices such as Chao, Shannon, and Simpson were different in the collected samples and solid samples (soil and rocks) showed higher taxon richness and evenness. Taxonomic diversity was unexpectedly higher and the major portion of sequences was assigned to Proteobacteria, Actinobacteria, and Acidobacteria. Higher variation in community structure was reported at the class level and Alphaproteobacteria was dominant. The solid niches were occupied by a higher number of phyla compared with liquid. The physicochemical variables acted as spatial gradients and associated with the bacterial structural communities of the glacial ecosystem. Findings showed that both Proteobacteria and Actinobacteria in solid samples influenced the bacterial community structure in downstream liquid samples. Interestingly, the metagenomic biomarkers were higher in liquid samples. This study provides precious datasets to understand the bacterial community in a better way under the influence of spatial, physical and environmental factors.     

Molybdenum supply increases root system growth of winter wheat by enhancing nitric oxide accumulation and expression of NRT genes

Plant and Soil - Success in agronomic biofortification of maize and wheat is highly variable. This study aimed to elucidate the differences in uptake and translocation of foliar-applied zinc (Zn)...     

Burr marigold (Bidens tripartita L.) roots directly and immediately scavenge rhizosphere methane with highly exuded hydrogen peroxide via a rhizosphere Fenton reaction

Plant and Soil - The major factors controlling the soil methane (CH4) concentration and CH4 emissions of various plant (mainly wetland) species were identified. Five plant species (Oryza sativa,...     

Recent advances on drug development and emerging therapeutic agents for Alzheimer’s disease

Molecular Biology Reports - Alzheimer’s disease (AD) is a neurodegenerative old age disease that is complex, multifactorial, unalterable, and progressive in nature. The currently approved...     

Characterization of extracellular vesicle miRNA identified in peripheral blood of chronic pancreatitis patients

Molecular and Cellular Biochemistry - Plasma-derived extracellular vesicles (EV) can serve as markers of cell damage/disease but can also have therapeutic utility depending on the nature of their...     

SARS-CoV-2 nucleocapsid and Nsp3 binding: an in silico study

Archives of Microbiology - Severe acute respiratory syndrome virus 2 (SARS-CoV-2) belongs to the single-stranded positive-sense RNA family. The virus contains a large genome that encodes four...