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991.
992.
993.
Isoprenoid quinones and fatty acids ofZoogloea 总被引:1,自引:0,他引:1
Akira Hiraishi Yong Kook Shin Junta Sugiyama Kazuo Komagata 《Antonie van Leeuwenhoek》1992,61(3):231-236
NineZoogloea strains including the type strain ofZ. ramigera (IAM 12136=ATCC 19544=N.C. Dondero 106) and newly isolated strains were investigated for isoprenoid quinone composition and whole-cell fatty acid profiles. Seven of the tested strains, having phenotypic properties typical ofZoogloea, were characterized by their production of both ubiquinone-8 and rhodoquinone-8 as major quinones, whereas the remaining two strains,Z. ramigera IAM 12669 (=K. Crabtree I-16-M) and IAM 12670 (=P.R. Dugan 115), formed ubiquinone-10 and ubiquinone-8, respectively, as the sole quinone. All rhodoquinone-producing strains contained palmitoleic acid and 3-hydroxy-decanoic acid as the major components of nonpolar and hydroxylated fatty acids, respectively. Marked differences were noted in the fatty acid composition between the strains with and without rhodoquinones. The chemotaxonomic data suggested that the rhodoquinonelacking strains should be excluded from the genusZoogloea. Since there have been no reliable taxonomic tools forZoogloea, rhodoquinone analysis may provide a new criterion of great promise for identifyingZoogloea strains. 相似文献
994.
Differences in the effect of vitamin E on nickel sulfide or nickel chloride-induced chromosomal aberrations in mammalian cells 总被引:1,自引:0,他引:1
Vitamin E (alpha-tocopherol succinate) pretreatment significantly inhibited formation of some but not all of the chromosomal aberrations induced by crystalline NiS particles but it did not affect the chromosome damage induced by NiCl2. Crystalline NiS particles are phagocytized by cells in contrast to water-soluble NiCl2 which enters the cells by a different mechanism. These and other previous studies suggest that the phagocytosis of crystalline NiS particles produces genetic damage as a consequence of oxygen radicals resulting from the irritant particle effect and also from the high intracellular levels of nickel ions dissolving from the endocytized particles. 相似文献
995.
996.
Amiloride, a specific inhibitor for the Na+-driven flagellar motors of alkalophilic Bacillus 总被引:8,自引:0,他引:8
Na+-driven flagellar motors of alkalophilic Bacillus were found to be inhibited by amiloride, a potent inhibitor for many Na+-coupled systems. A concentration of 0.5 mM of amiloride completely inhibited motility but showed almost no effect on the membrane potential, the intracellular pH homeostasis, and the ATP content of the cells. Furthermore, the activity of a Na+-coupled amino acid transport system was reduced only by half by this concentration of amiloride. Thus, the inhibition of motility of alkalophilic Bacillus by amiloride was rather specific. The inhibition of motility produced by amiloride was restored by increasing Na+ concentrations in the medium. Kinetic analysis of the data revealed that the inhibition was competitive with respect to the concentration of Na+ in the medium. Therefore, it is quite logical to assume that amiloride inhibits the rotation of the Na+-driven flagellar motors of alkalophilic Bacillus by competing with Na+ at the force-generating site of the motor. Some amiloride analogs known to selectively inhibit Na+ channels were potent inhibitors for the flagellar motors, suggesting that the Na+-interacting site of the motors has some similarity to that of the Na+ channels. 相似文献
997.
Chemistry of the alkali-labile lesion formed from iron(II) bleomycin and d(CGCTTTAAAGCG) 总被引:3,自引:0,他引:3
Two sets of products are formed from DNA upon treatment with Fe(II).bleomycin + O2. One set, which is believed to derive from a C-4' hydroperoxy derivative of the DNA deoxyribose moiety, includes the four possible base propenals, as well as DNA oligomers having deoxynucleoside 3'-(phosphoro-2"-O-glycolates) at their 3'-termini. The other set of products consists of free bases and alkali-labile lesions, the latter of which had not previously been characterized structurally. By use of the self-complementary dodecanucleotide d(CGCTTTAAAGCG) having a site modified by Fe-bleomycin three nucleotides from the 5'-end, it has been possible to characterize the alkali-labile product as a C-4' hydroxyapurinic acid. When the bleomycin-treated dodecanucleotide was treated with agents that effected decomposition of the alkali-labile lesion, products of the form CpGpx were obtained, and these proved useful for structural characterization of the alkali-labile lesion. Treatment with alkali produced CpGpx, where x was 2,4-dihydroxycyclopentenone. Alternatively, treatment with hydrazine provided a pyridazine derivative, and aqueous alkylamines led to formation of CpGp itself. The structures of all dinucleotides produced from the alkali-labile lesion were verified by direct comparison with authentic synthetic samples. 相似文献
998.
The DJH complex remains active in recombination to VH segments after the loss of mu-chain expression in mu-positive pre-B cells 总被引:2,自引:0,他引:2
T Maeda H Sugiyama Y Tani S Kishimoto 《Journal of immunology (Baltimore, Md. : 1950)》1989,142(10):3652-3656
AT11-2 is an Abelson virus-transformed B precursor cell line which is capable of differentiating Ig- from mu+ cells via functional recombination of VH segments to preexisting DJH complexes. We describe here that after a mu+ subclone (VDJ+/DJ) generated from Ig- AT11-2 (DJ/DJ) cells by in vitro functional VH to DJH recombination subsequently lost mu-chain expression either by the recombination of a pseudo VH segment to the VHDJH+ allele or by the deletion of VHDJH+ allele, a novel productive joining of VH segments to the preexisting DJH complex occurred. These results indicated that VH to VHDJH rearrangement was not suppressed in mu-chain producing cells and that the DJH complexes still remained active in the recombination to VH segments after the loss of mu-chain expression. Our results may also suggest that VH to DJH rearrangement, but not VH to VHDJH rearrangement, is suppressed in mu-chain producing cells to maintain allelic exclusion. Our cell differentiation system should continue to be valuable for elucidating the mechanism of suppression and associated implications regarding allelic exclusion. 相似文献
999.
Fusakazu Misoka Tetsuo Miyake Ken-ichi Miyoshi Masanori Sugiyama Shunji Sakamoto Toru Fuwa 《Biotechnology letters》1989,11(12):839-844
Summary Human insulin-like growth factor II (hIGF-II) was produced inEscherichia
coli as a protein fused to human growth hormone. High level expression of the fusion protein was attained with pIBL-1 plasmid. The hIGF-II obtained byin
vitro cleavage of the fusion protein with cyanogen bromide was highly purified and its biological activity was assessed. 相似文献
1000.
Rabbit antiserum against rat plasma fibronectin induced histamine release in isolated rat peritoneal mast cells. Immunofluorescence revealed fibronectin on the mast cells in rat mesentery and on the surface of the isolated mast cells. Mast cells adhered to collagen-coated dishes. This cellular adherence was inhibited by the addition of anti-fibronectin. Fibronectin on the surface of mast cells may play a role of attachment of the cells to collagenous connective tissues. 相似文献