排序方式: 共有94条查询结果,搜索用时 234 毫秒
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Use of nitrate to control sulfide generation by sulfate-reducing bacteria associated with oily waste
Received 13 November 1998/ Accepted in revised form 03 March 1999 相似文献
83.
D.B. Ringelberg G.T. Townsend K.A. DeWeerd J.M. Suflita D.C. White 《FEMS microbiology ecology》1994,14(1):9-18
Abstract Desulfomonile tiedjei is a Gram-negative sulfate-reducing bacterium capable of catalyzing aryl reductive dehalogenation reactions. Since many toxic and persistent contaminants in the subsurface are halogenated aromatic compounds, the detection and enumeration of dehalogenating microorganisms in the environment may be a useful tool for planning and evaluating bioremediation efforts. In this study, we show that D. tiedjei contains unique lipopolysaccharide branched 3-hydroxy fatty acids, unknown as yet in other bacteria, and that it is possible to detect the bacterium in inoculated aquifer sediments based on these signature lipid biomarkers. The detection of D. tiedjei and other dehalogenating microorganisms possessing similar cellular properties in environmental matrices may be possible by this technique. Additionally, the effect of such inoculation on dehalogenation activity is examined. 相似文献
84.
Characterization of the acclimation period before anaerobic dehalogenation of halobenzoates. 总被引:8,自引:7,他引:1
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The acclimation periods prior to detectable dehalogenation of halogenated benzoates in anaerobic lake sediments ranged from 3 weeks to 6 months. These acclimation periods were reproducible over time and among sampling sites and were characteristic of the chemical tested. The lengthy acclimation period appears to represent an induction phase in which little or no aryl dehalogenation is observed, followed by an exponential increase in activity typical of an enrichment response. Continuous growth from the time of the first exposure to the chemical is inconsistent with the extremely low per-cell activities estimated for the early days of the acclimation period and the fact that the dehalogenation yields no carbon to support microbial growth. The finding of a characteristic acclimation time for each chemical argues against nutritional deficiency, inhibition, or predation as an explanation for this phase of metabolism, while the reproducibility of the findings with time and space and among replicates argues against genetic changes as the explanation. The acclimation times did correlate with the eventual dehalogenation rates. This may reflect the general energy limitations in the anaerobic communities and suggests that those chemicals with faster dehalogenation rates provide more energy for the induction and growth phases of the active population. 相似文献
85.
Strevett Keith Davidova Irene Suflita Joseph M. 《Reviews in Environmental Science and Biotechnology》2002,1(2):143-167
A biodegradability assay should mimic in situ conditions as closely as possible. If this is not entirely possible, the assayshould at least include inoculum from the site. This review attemptsto condense current literature on anaerobic biodegradability assayand propose a clear assessment methodology to determine the fatesurfactants in anaerobic environments. It has been well documentedthat surfactant concentrations toxic to the microflora can lead tounwarranted failure of biodegradability assays. Thus, an important recommendation is to first perform a toxicity evaluation with relevant controls. Based on the results of this evaluation, a Tier 1biodegradability assay that assesses the rate of formation of reducedendproducts or the consumption of a particular terminal electron acceptor is recommended and supported by current literature. Balancedchemical equations for the complete mineralization of the substrateare then used to compare the amount of transformation that actuallyoccurred with that theoretically expected. When required, resultsshould be confirmed by Tier 2 testing, which includes monitoring ofsubstrate disappearance over time using a variety of analytical tools.These recommended procedures are scientifically defensible and havethe potential of providing environmentally relevant information on thefate of surfactant materials in the environment. 相似文献
86.
Extrapolation of biodegradation results to groundwater aquifers: reductive dehalogenation of aromatic compounds 总被引:15,自引:0,他引:15
The reductive biodegradation of a variety of haloaromatic substrates was monitored in samples from two sites within a shallow anoxic aquifer and was compared with freshwater sediment and sewage sludge. The metabolic capacity existing in methane-producing aquifer material was very similar to that in sediment in that three of four chlorobenzoates, five of seven chlorophenols, and one of two chlorophenoxyacetate herbicides were reductively dehalogenated in both types of incubations. The 2,4-dichlorophenoxyacetate was first converted to a dichlorophenol before dehalogenation occurred. Sewage sludge microorganisms dehalogenated four of seven chlorophenols tested and degraded both phenoxyacetate herbicides by first converting them to the corresponding chlorophenols, but the microorganisms did not transform the chlorobenzoates. In general, the same suite of initial metabolites were produced from a test substrate in all types of samples, as confirmed by cochromatography of the intermediates with authentic material. Aquifer microbiota from a sulfate-reducing site was unable to significantly degrade any of the haloaromatic substrates tested. Biological removal of the sulfate in samples from this site permitted dehalogenation of a model substrate, while stimulation of methanogenesis without removal of sulfate did not. These results demonstrate that dehalogenating microorganisms were present at this site but that their activity was at least partially inhibited by the high sulfate levels. 相似文献
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L R Krumholz S H Harris S T Tay J M Suflita 《Applied and environmental microbiology》1999,65(6):2300-2306
We examined the relative roles of acetogenic and sulfate-reducing bacteria in H2 consumption in a previously characterized subsurface sandstone ecosystem. Enrichment cultures originally inoculated with ground sandstone material obtained from a Cretaceous formation in central New Mexico were grown with hydrogen in a mineral medium supplemented with 0.02% yeast extract. Sulfate reduction and acetogenesis occurred in these cultures, and the two most abundant organisms carrying out the reactions were isolated. Based on 16S rRNA analysis data and on substrate utilization patterns, these organisms were named Desulfomicrobium hypogeium sp. nov. and Acetobacterium psammolithicum sp. nov. The steady-state H2 concentrations measured in sandstone-sediment slurries (threshold concentration, 5 nM), in pure cultures of sulfate reducers (threshold concentration, 2 nM), and in pure cultures of acetogens (threshold concentrations 195 to 414 nM) suggest that sulfate reduction is the dominant terminal electron-accepting process in the ecosystem examined. In an experiment in which direct competition for H2 between D. hypogeium and A. psammolithicum was examined, sulfate reduction was the dominant process. 相似文献
90.
Summary The ability ofAcetobacterium woodii andEubacterium limosum to degrade methyl esters of acetate, propionate, butyrate, and isobutyrate was examined under growing and resting-cell conditions. Both bacteria hydrolyzed the esters to the corresponding carboxylates and methanol under either condition. Methanol was further oxidized to formate under growing but not resting conditions. Unlike the metabolism of phenylmethylethers, no H2 requirement was evident for ester biotransformation. The hydrolysis of methyl carboxylates is thermodynamically favorable under standard conditions and the mixotrophic metabolism of ester/CO2 allowed for bacterial growth. These results suggest that the degradation of methyl carboxylates may be a heretofore unrecognized nutritional option for acetogenic bacteria. 相似文献