全文获取类型
收费全文 | 1834篇 |
免费 | 229篇 |
国内免费 | 11篇 |
出版年
2023年 | 7篇 |
2022年 | 11篇 |
2021年 | 23篇 |
2020年 | 33篇 |
2019年 | 65篇 |
2018年 | 57篇 |
2017年 | 28篇 |
2016年 | 54篇 |
2015年 | 71篇 |
2014年 | 87篇 |
2013年 | 119篇 |
2012年 | 126篇 |
2011年 | 82篇 |
2010年 | 69篇 |
2009年 | 62篇 |
2008年 | 90篇 |
2007年 | 88篇 |
2006年 | 93篇 |
2005年 | 60篇 |
2004年 | 65篇 |
2003年 | 61篇 |
2002年 | 72篇 |
2001年 | 60篇 |
2000年 | 40篇 |
1999年 | 50篇 |
1998年 | 29篇 |
1997年 | 22篇 |
1996年 | 31篇 |
1995年 | 29篇 |
1994年 | 11篇 |
1993年 | 18篇 |
1992年 | 26篇 |
1991年 | 29篇 |
1990年 | 25篇 |
1989年 | 28篇 |
1988年 | 13篇 |
1987年 | 14篇 |
1986年 | 20篇 |
1985年 | 11篇 |
1984年 | 21篇 |
1983年 | 13篇 |
1982年 | 8篇 |
1980年 | 17篇 |
1979年 | 11篇 |
1978年 | 18篇 |
1977年 | 10篇 |
1975年 | 21篇 |
1974年 | 14篇 |
1973年 | 18篇 |
1972年 | 9篇 |
排序方式: 共有2074条查询结果,搜索用时 31 毫秒
101.
Yoshiko Iida I‐Fang Sun Charles A. Price Chien‐Teh Chen Zueng‐Sang Chen Jyh‐Min Chiang Chun‐Lin Huang Nathan G. Swenson 《Ecology and evolution》2016,6(17):6085-6096
A fundamental goal in ecology is to link variation in species function to performance, but functional trait–performance investigations have had mixed success. This indicates that less commonly measured functional traits may more clearly elucidate trait–performance relationships. Despite the potential importance of leaf vein traits, which are expected to be related to resource delivery rates and photosynthetic capacity, there are few studies, which examine associations between these traits and demographic performance in communities. Here, we examined the associations between species traits including leaf venation traits and demographic rates (Relative Growth Rate, RGR and mortality) as well as the spatial distributions of traits along soil environment for 54 co‐occurring species in a subtropical forest. Size‐related changes in demographic rates were estimated using a hierarchical Bayesian approach. Next, Kendall's rank correlations were quantified between traits and estimated demographic rates at a given size and between traits and species‐average soil environment. Species with denser venation, smaller areoles, less succulent, or thinner leaves showed higher RGR for a wide range of size classes. Species with leaves of denser veins, larger area, cheaper construction costs or thinner, or low‐density wood were associated with high mortality rates only in small size classes. Lastly, contrary to our expectations, acquisitive traits were not related to resource‐rich edaphic conditions. This study shows that leaf vein traits are weakly, but significantly related to tree demographic performance together with other species traits. Because leaf traits associated with an acquisitive strategy such as denser venation, less succulence, and thinner leaves showed higher growth rate, but similar leaf traits were not associated with mortality, different pathways may shape species growth and survival. This study suggests that we are still not measuring some of key traits related to resource‐use strategies, which dictate the demography and distributions of species. 相似文献
102.
Activation of brain steroidogenesis and neurogenesis during the gonadal differentiation in protandrous black porgy,Acanthopagrus schlegelii
下载免费PDF全文
![点击此处可从《Developmental neurobiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Chien‐Ju Lin Yi‐Chun Fan‐Chiang Sylvie Dufour Ching‐Fong Chang 《Developmental neurobiology》2016,76(2):121-136
The early brain development, at the time of gonadal differentiation was investigated using a protandrous teleost, black porgy. This natural model of monosex juvenile fish avoids the potential complexity of sexual dimorphism. Brain neurogenesis was evaluated by histological analyses of the diencephalon, at the time of testicular differentiation (in fish between 90 and 150 days after hatching). Increases in the number of both Nissl‐stained total brain cells, and Pcna‐immunostained proliferative brain cells were observed in specific area of the diencephalon, such as ventromedialis thalami and posterior preoptic area, revealing brain cell proliferation. qPCR analyses showed significantly higher expression of the radial glial cell marker blbp and neuron marker bdnf. Strong immunohistochemical staining of Blbp and extended cellular projections were observed. A peak expression of aromatase (cyp19a1b), as well as an increase in estradiol (E2) content were also detected in the early brain. These data demonstrate that during gonadal differentiation, the early brain exhibits increased E2 synthesis, cell proliferation, and neurogenesis. To investigate the role of E2 in early brain, undifferentiated fish were treated with E2 or aromatase inhibitor (AI). E2 treatment upregulated brain cyp19a1b and blbp expression, and enhanced brain cell proliferation. Conversely, AI reduced brain cell proliferation. Castration experiment did not influence the brain gene expression patterns and the brain cell number. Our data clearly support E2 biosynthesis in the early brain, and that brain E2 induces neurogenesis. These peak activity patterns in the early brain occur at the time of gonad differentiation but are independent of the gonads. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 121–136, 2016 相似文献
103.
BackgroundEqual access to healthcare facilities and high-level quality of care are important strategies to eliminate the disparity in outcome of care. However, the existing literature regarding how urban or rural dwelling patients with different income level select healthcare providers is insufficient. The purposes of this study were to examine whether differences of healthcare provider selection exist among urban and rural coronary artery bypass surgery (CABG) patients with different income level. If so, we further investigated the associated impact on mortality.MethodsA retrospective, multilevel study design was conducted using claims data from 2007–2011 Taiwan’s Universal Health Insurance Scheme. Healthcare providers’ performance and patients’ travelling distance to hospitals were used to define the patterns of healthcare provider selection. Baron and Kenny’s procedures for mediation effect were conducted.ResultsThere were 10,108 CABG surgeries included in this study. The results showed that urban dwelling and higher income patients were prone to receive care from better-performance providers. The travelling distances of urban dwelling patients was 15 KM shorter, especially when they received better-performance provider’s care. The results also showed that the difference of healthcare provider selection and mortality rate existed between rural and urban dwelling patients with different income levels. After the procedure of mediation effect testing, the results showed that the healthcare provider selection partially mediated the relationships between patients’ residential areas with different income levels and 30-day mortality.ConclusionPreferences of healthcare provider selection vary among rural and urban patients with different income, and such differences partially mediated the outcome of care. Health authorities should pay attention to this issue, and propose appropriate solutions to eliminate the disparity in outcome of CABG care. 相似文献
104.
Shin H. Haga J. H. Kosawada T. Kimura K. Li Y. S. Chien S. Schmid-Schönbein G. W. 《Biomechanics and modeling in mechanobiology》2019,18(1):5-16
Biomechanics and Modeling in Mechanobiology - Recent experimental evidence points to the possibility that cell surface-associated caveolae may participate in mechanotransduction. The particular... 相似文献
105.
106.
Shu Chien 《Molecular & cellular biomechanics : MCB》2019,16(3):163-178
Professor Y.C. Fung has made tremendous impacts on science, engineering and humanity through his research and its applications, by setting the highest standards, through educating many students and their students, and providing his exemplary leadership. He has applied his profound knowledge and elegant analytical methods to the study of biomedical problems with rigor and excellence. He established the foundations of biomechanics in living tissues and organs. Through his vision of the power of “making models” to explain and predict biological phenomena, Dr. Fung opened up new vista for bioengineering, from organs-systems to molecules-genes, and has provided the foundation of research activities in many institutions in the United States and the world. He has made outstanding contributions to education in bioengineering, service to professional organizations, and translation to industry and clinical medicine. He is widely recognized as the Father of Biomechanics and the leading Bioengineer in the world. His extraordinary achievements and commands in science, engineering and the arts make him a Renaissance Man for the world. 相似文献
107.
108.
The renewal and differentiation of Isl1+ cardiovascular progenitors are controlled by a Wnt/beta-catenin pathway 总被引:3,自引:0,他引:3
Qyang Y Martin-Puig S Chiravuri M Chen S Xu H Bu L Jiang X Lin L Granger A Moretti A Caron L Wu X Clarke J Taketo MM Laugwitz KL Moon RT Gruber P Evans SM Ding S Chien KR 《Cell Stem Cell》2007,1(2):165-179
Isl1(+) cardiovascular progenitors and their downstream progeny play a pivotal role in cardiogenesis and lineage diversification of the heart. The mechanisms that control their renewal and differentiation are largely unknown. Herein, we show that the Wnt/beta-catenin pathway is a major component by which cardiac mesenchymal cells modulate the prespecification, renewal, and differentiation of isl1(+) cardiovascular progenitors. This microenvironment can be reconstituted by a Wnt3a-secreting feeder layer with ES cell-derived, embryonic, and postnatal isl1(+) cardiovascular progenitors. In vivo activation of beta-catenin signaling in isl1(+) progenitors of the secondary heart field leads to their massive accumulation, inhibition of differentiation, and outflow tract (OFT) morphogenic defects. In addition, the mitosis rate in OFT myocytes is significantly reduced following beta-catenin deletion in isl1(+) precursors. Agents that manipulate Wnt signals can markedly expand isl1(+) progenitors from human neonatal hearts, a key advance toward the cloning of human isl1(+) heart progenitors. 相似文献
109.
In this study, we used nanocomposite magnetic particles coated with alumina as the affinity probes to selectively concentrate phosphorylated peptides and proteins from a low volume of sample solution. Tryptic digest products of phosphoproteins including alpha and beta-caseins, human protein phosphatase inhibitor 1, nonfat milk, egg white, and a cell lysate were used as the samples to demonstrate the feasibility of this approach. In only 30 and 90 s, phosphopeptides and phosphoproteins sufficient for characterization by MALDI-MS were enriched by the particles, respectively. Proteins trapped on the particles could be directly digested on the particles. The same particles in the digest solution were employed for enrichment of phosphopeptides. We estimated the required time for performing the enrichment of phosphopeptides from complex samples and characterization by MALDI MS was within 5 min. A small volume (50 microL) and a low concentration (5 x 10(-10) M) of tryptic digest product of a phosphoprotein sample could be dramatically enriched and characterized using this approach. 相似文献
110.