睾丸间质细胞—研究自体吞噬的一种正常细胞模型

In the present study, we tried to estimate, in a semiquantitative way, the relative frequency of the autophagic activity in various cell types under physiological condition. The results indicated that the highest activity appeared to be in the Leydig cells of rat testes. Autophagosomes were frequently observed in electron microscope photographs of Leydig cells, which provide a good model to study the autophagocytosis in normal cells. The autophagic process in Leydig cells was observed with the electron microscope in preparations treated to show CMPase activity. The mode of formation of autophagosomes in Leydig cells can be divided into three steps. Step 1, flattened membranous elements expand to enclose a small cytoplasmic territory to form pre-autophagosome. Step 2, The double membrane profile of the pre-autophagosome then completely encloses the cytoplasmic territory to form early autophagosome in which structurally normal organelles are contained. Step 3, the transformation of an early autophagosome into a late one is accompanied by the loss of one of the two delimiting membranes, the partial disintegration of the enclosed content and simultaneous acquisition of acid phosphatase activity. The enzymatic reactivity is acquired following a close association with the lysosomes. The late autophagosome then reaches the cell surface and appear to exocytose their residual content.     

A procedure for the construction of linear restriction fragment maps of a 50- to 100-kb DNA.

A simple and effective procedure for the construction of linear restriction fragment maps was developed. Using a two-enzyme digestion, two-dimensional (2-D) electrophoresis procedure, all the restriction fragments in a 50- to 100-kb DNA can be individually resolved and displayed on a 2-D plane. This 2-D gel pattern, with appropriate markers, provides a fixed set of x, y coordinates for each fragment obtained from the single and double digestion as well as the relationship between the two steps. A matrix is constructed from the 2-D pattern. The vertical column shows all the singly digested individual fragments and their sizes obtained from each restriction enzyme treatment, and the dividing horizontal row shows all the doubly digested DNA fragments and their sizes after treatment with two enzymes. The order of arrangement is always from the smallest to the largest fragments. Using this matrix, two linear DNA restriction maps for these two enzymes can be simultaneously constructed in a self-reconfirming manner. As examples for this procedure, we describe the construction of two linear restriction fragment maps, a combination of EcoRI and BamHI digestion as well as a combination of EcoRI and HindIII digestion of lambda-phage DNA.     

应用纯化的重组Epstein-Barr病毒早期抗原建立检测鼻咽癌病人血清IgA/EA抗体的ELISA方法

利用凝胶和离子交换柱(Mono Q)两次层析,将大肠杆菌表达的EB病毒早期抗原P138片段多肽纯化。以此P138为抗原,增加鼠抗人IgA单克隆抗体以扩大IgA的反应,建立了三步ELISA法。用本法检查了100例鼻咽癌病人和63例正常人血清中抗EB病毒IgA/EA抗体,病人血清的阳性检出率为86％,正常人有3例阳性(4.7％)。此结果表明,三步ELISA法较常用的间接ELISA法(阳性检出率为71％)敏感。     

外源脂肪酸及胆固醇对莱氏衣原体膜糖脂含量的影响

 <正> 单葡萄糖甘油二酯(MGDG)和双葡萄糖甘油二酯(DGDG)是莱氏衣原体膜上主要的极性脂。糖脂的生理功能过去很少研究,至今仍不清楚。近年来实验结果表明,MGDG在膜上容易形成六角形Ⅱ结构,而DGDG则形成脂双层结构。六角形Ⅱ结构的出现与生物膜的生理功能的关系是当前瞩目的研究内容。本文首次研究了外源脂肪酸和胆固酵对莱氏衣原体AIH089菌株膜上糖脂含量的影响。     

Influence of BA and sucrose on the competence and determination of pepper (Capsicum annuum L. var. Sweet Banana) hypocotyl cultures during shoot formation

Summary The simultaneous presence of 6-benzyladenine (BA) and sucrose in a Murashige and Skoog medium (SIM) during the initial stages of shoot initiation have been found to be obligatory for high-frequency shoot formation in the Capsicum annuum L. var. Sweet Banana upper hypocotyl explants. The explants are determined for shoot formation following a minimum of 8 days of culture on SIM. Deprivation of exogenous sucrose from day 6 to day 20 of culture had no effect on the shoot forming response of the explants. BA and sucrose appear to act independently on different aspects of the competence of explants to respond to SIM during shoot initiation.Abbreviations BA N⁶-benzyladenine - MS Murashige and Skoog medium - SIM shoot induction medium - HFM hormone free medium - SUC sucrose minus medium     

大豆子叶内酸性磷酸酶活性的超微结构定位

开花后３５～５０ ｄ 期间和萌发早期（播种后４～８ ｄ）的大豆（Ｇｌｙｃｉｎｅｍ ａｘ Ｌ．）种子中,酸性磷酸酶主要分布在子叶细胞中的蛋白体内;在内质网内也检测到酸性磷酸酶活性。此外,在萌发早期的部分子叶细胞的质膜外侧及其细胞壁基质中可见密集的酸性磷酸酶活性;而且在近质膜的胞质中常见到一些富含磷酸铅沉淀的胞质小泡,似与质膜融合     

福建武夷山甜槠群落能量的研究

在生物量、生产力研究基础上,对武夷山甜槠（Ｃａｓｔａｎｏｐｓｉｓ ｅｙｒｅｉ（Ｃｈａｍ ｐ．ｅｘ Ｂｅｎｔｈ．） Ｔｕｔｃｈ．）群落各组分的热值、群落能量现存量、能量年净固定量以及太阳能转化效率进行了研究。结果表明：（１）甜槠群落各组分样品的干重热值具有一定的差异,树皮热值最高,细根热值最低。（２）甜槠群落的能量现存量达７８０５８４．１ ｋＪ·ｍ － ２,其中地上部分为６７８９１３．８ ｋＪ·ｍ － ２,占总量的８６．９８％ ;地下部分为１０１６７０．３ ｋＪ·ｍ － ２,占１３．０２％ 。（３）甜槠群落的能量年净固定量（１９９２年）为２６８５６．２ ｋＪ·ｍ － ２·ａ－ １,林地太阳光合有效辐射能的转化效率为１．２９６％ 。