排序方式: 共有27条查询结果,搜索用时 15 毫秒
21.
Fujitani Noboru Kawaguchi Naomasa Toda Shuji Matsumura Sueo Kimura Hiroshi Onishi Shunzo 《Molecular and cellular biochemistry》1997,169(1-2):73-78
An immunocytochemical study was performed to examine the expression of cellular c-myc protein in the heart of 30-, 120- and 180-day-old cardiomyopathic Syrian UM-X7.1 hamsters. The heart of age- and sex-matched BIO-RB hamster was used as normal control. In paraffin sections, an immunostaining for c-myc was markedly increased in cytoplasm of cells from the UM-X7.1 heart as compared with that of the BIO-RB heart which showed a weak staining. However, c-myc was localized in nuclei of cells in frozen sections of the heart. Specific cell types of the heart were differentiated with anti-vimentin, and we found that the increased expression of c-myc was present in nuclei of muscle cells of the UM-X7.1 myocardium. A quantitative study of c-myc-positive nuclei of muscle and nonmuscle cells was carried out by a video micrometer. The mean number of c-myc-positive nuclei of muscle cells was significantly higher in the cardiomyopathic heart than in the control heart from hamsters of all ages studied. These results suggest that the increase of c-myc protein may relate to the pathological state or pathogenesis of the hereditary cardiomyopathy. 相似文献
22.
Maksut Coşkun Akiyo Sakushima Sansei Nishibe Sueo Hisada Nevin Tanker 《Phytochemistry》1982,21(6):1453-1454
A new phloroglucinol derivative, abbreviatin BB, has been isolated from Dryopteris abbreviata. Its structure was elucidated to be methylene-bis-met 相似文献
23.
Hiroki Tsukamoto Sueo Hisada Sansei Nishibe David G. Roux John P. Rourke 《Phytochemistry》1984,23(3):699-700
Esculetin and scopoletin were isolated from the bark of Olea africana while isoscopoletin and scoparone were isolated from the bark of Olea capensis. The distribution of these coumarins in Olea species from South Africa is described. 相似文献
24.
Kano Y Horie N Doi S Aramaki F Maeda H Hiragami F Kawamura K Motoda H Koike Y Akiyama J Eguchi S Hashimoto K 《Neurochemical research》2008,33(9):1795-1803
We investigated whether artepillin C, a major component of Brazilian propolis, acts as a neurotrophic-like factor in rat PC12m3
cells, in which nerve growth factor (NGF)-induced neurite outgrowth is impaired. When cultures of PC12m3 cells were treated
with artepillin C at a concentration of 20 μM, the frequency of neurite outgrowth induced by artepillin C was approximately
7-fold greater than that induced by NGF alone. Artepillin C induced-neurite outgrowth of PC12m3 cells was inhibited by the
ERK inhibitor U0126 and by the p38 MAPK inhibitor SB203580. Although artepillin C-induced p38 MAPK activity was detected in
PC12m3 cells, phosphorylation of ERK induced by artepillin C was not observed. On the other hand, artepillin C caused rapid
activation of ERK and the time course of the activation was similar to that induced by NGF treatment in PC12 parental cells.
However, NGF-induced neurite outgrowth was inhibited by artepillin C treatment. Interestingly, inhibition of ERK by U0126
completely prevented artepillin C-induced p38 MAPK phosphorylation of PC12m3 cells. These findings suggest that artepillin
C-induced activation of p38 MAPK through the ERK signaling pathway is responsible for the neurite outgrowth of PC12m3 cells. 相似文献
25.
Sueo Urushizaki Yasuo Ota Tetsuya Haruyama Hiromasa Kano Kuniomi Matsumoto Minoru Haga 《Bioscience, biotechnology, and biochemistry》2013,77(12):3159-3161
To find amino acid residues which are required for glucoamylase activity, mutant glucoamylase genes were constructed by in vitro mutations of GLU1 DNA encoding Saccharomycopsis fibuligera glucoamylase and introduced into Saccharomyces cerevisiae, and the resulting mutant proteins were assayed for enzymatic activities. Eighteen mutant proteins were obtained by random insertions of a BamHX linker DNA. Six out of 7 proteins with mutations in conserved regions among divergent glucoamylases showed no activities, while 8 out of 11 proteins with mutations in unconserved regions had similar specific activities to a wild-type value, suggesting that the conserved regions are important to the activity. A series of amino-terminal deletion mutants were also constructed. A mutant protein with a deletion of only two amino acid residues from the amino terminus had a significant reduction in the activity, suggesting an essential role for the amino-terminal peptide. Ten mutant proteins with single amino acid replacements were produced by site-directed mutagenesis. Analyses for thermal stability and temperature dependency of these mutant proteins revealed that Ala81, Asp89, Trp94, Arg96, Asp97, and Trp166 are required for wild-type levels of activities, and that at least Ala81 and Asp89 are not essential to catalytic activities, but act in thermal stability. 相似文献
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27.
Yuko Tashiro Sueo Matsumura Noriko Murakami Akira Kumon 《Archives of biochemistry and biophysics》1984,233(2):540-546
The 20-kDa light chain isolated from gizzard myosin has recently been reported to be phosphorylated by casein kinase II at a site distinct from that phosphorylated by Ca2+- and calmodulin-dependent myosin light-chain kinase. In the present study, the site phosphorylated by casein kinase II has been analyzed through procedures including tryptic digestion of the radioactively phosphorylated light chain and CNBr cleavage of the purified tryptic phosphopeptide, followed by amino acid analysis of these phosphopeptides. Comparison of the amino acid compositions of these peptides with the previously reported sequence has indicated that the phosphorylation site is threonine-134 of the light chain. The significance of the phosphorylation of the light chain by casein kinase II, as well as the substrate specificity of the protein kinase, is discussed on the basis of the result. 相似文献