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51.
Chiranjeevi Tikka Hari Prasad Osuru Navya Atluri Praveen Chakravarthi Veera Raghavulu Nanda Kumar yellapu Ismail Shaik Mannur Uppu Venkateswara Prasad Sudheer Aluru Narasimha Varma K Matcha Bhaskar 《Bioinformation》2013,9(8):421-425
Yeast strains are commonly associated with sugar rich environments. Various fruit samples were selected as source for isolating
yeast cells. The isolated cultures were identified at Genus level by colony morphology, biochemical characteristics and cell
morphological characters. An attempt has been made to check the viability of yeast cells under different concentrations of ethanol.
Ethanol tolerance of each strain was studied by allowing the yeast to grow in liquid YEPD (Yeast Extract Peptone Dextrose)
medium having different concentrations of ethanol. A total of fifteen yeast strains isolated from different samples were used for the
study. Seven strains of Saccharomyces cerevisiae obtained from different fruit sources were screened for ethanol tolerance. The
results obtained in this study show a range of tolerance levels between 7%-12% in all the stains. Further, the cluster analysis based
on 22 RAPD (Random Amplified polymorphic DNA) bands revealed polymorphisms in these seven Saccharomyces strains. 相似文献
52.
Jiandong Zheng Xixiang Huo Yang Huai Lin Xiao Hui Jiang John Klena Carolyn M. Greene Xuesen Xing Jigui Huang Shali Liu Youxing Peng Hui Yang Jun Luo Zhibin Peng Linlin Liu Maoyi Chen Hui Chen Yuzhi Zhang Danqin Huang Xuhua Guan Luzhao Feng Faxian Zhan Dale J. Hu Jay K. Varma Hongjie Yu 《PloS one》2016,11(3)
53.
Imteyaz Ahmad Khan Sucharita Pilli Surendranath A Ritika Rampal Sudhir Kumar Chauhan Veena Tiwari Venigalla Pratap Mouli Saurabh Kedia Baibaswata Nayak Prasenjit Das Govind K. Makharia Vineet Ahuja 《PloS one》2016,11(3)
Background
Association of Mycobacterium avium subspecies paratuberculosis (MAP) and Crohn’s disease (CD) has been controversial due to contradictory reports. Therefore, we determined the prevalence of MAP in patients with CD and intestinal tuberculosis (ITB) and its association with clinical course.Methodology
Blood and intestinal biopsies were taken from 69 CD, 32 ITB patients and 41 patients with haemorrhoidal bleed who served as controls. qPCR targeting of MAP-specific IS900 gene was used to detect the presence of MAP DNA. qPCR results were further validated by sequencing. Immunohistochemistry (IHC) was used to detect the presence of MAP antigen in biopsy specimens. CD and ITB patients were followed-up for disease course and response to therapy.Principal Findings
The frequency of MAP-specific DNA in biopsies by qPCR was significantly higher in CD patients (23.2%, p = 0.03) as compared to controls (7.3%). No significant difference in intestinal MAP presence was observed between ITB patients (12.5%, p = 0.6) and controls (7.3%). MAP presence in blood of CD patients was 10.1% as compared to 4.9% in controls while no patients with ITB were found to be positive (p = 0.1). Using IHC for detection of MAP antigen, the prevalence of MAP in CD was 2.9%, 12.5% in ITB patients and 2.4% in controls. However, long-term follow-up of the patients revealed no significant associations between clinical characteristics and treatment outcomes with MAP positivity.Conclusion
We report significantly high prevalence of MAP in intestinal biopsies of CD patients. However, the presence of MAP does not affect the disease course and treatment outcomes in either CD or ITB patients. 相似文献54.
Hui Wang Tujin Shi Wei-Jun Qian Tao Liu Jacob Kagan Sudhir Srivastava 《Expert review of proteomics》2016,13(1):99-114
Mass spectrometry (MS) -based proteomics has become an indispensable tool with broad applications in systems biology and biomedical research. With recent advances in liquid chromatography (LC) and MS instrumentation, LC–MS is making increasingly significant contributions to clinical applications, especially in the area of cancer biomarker discovery and verification. To overcome challenges associated with analyses of clinical samples (for example, a wide dynamic range of protein concentrations in bodily fluids and the need to perform high throughput and accurate quantification of candidate biomarker proteins), significant efforts have been devoted to improve the overall performance of LC–MS-based clinical proteomics platforms. Reviewed here are the recent advances in LC–MS and its applications in cancer biomarker discovery and quantification, along with the potentials, limitations and future perspectives. 相似文献
55.
Tanushri Chatterji Suruchi Singh Manodeep Sen Ajai Kumar Singh Pradeep Kumar Maurya Nuzhat Husain Janmejai Kumar Srivastava Sudhir Kumar Mandal Raja Roy 《Metabolomics : Official journal of the Metabolomic Society》2016,12(8):130
Introduction
Meningitis, a morbidly infectious central nervous system pathology is accompanied by acute inflammation of the meninges, causing raised intracranial pressure linked with serious neurological sequelae.Objective
To observe the variation in the metabolic profile, that may occur in serum and urine along with CSF in adults using 1H NMR spectroscopy, with an attempt of appropriate and timely treatment regimen.Methods
The 1H NMR-based metabolomics has been performed in 115 adult subjects for differentiating bacterial meningitis (BM) and tubercular meningitis (TBM).Results
The discriminant function analysis (DFA) of the three bio-fluids collectively identified 3-hydroxyisovalerate, lactate, glucose, formate, valine, alanine, ketonic bodies, malonate and choline containing compounds (choline and GPC) as significant metabolites among cases versus control group. The differentiation of bacterial meningitis and tuberculous meningitis (BM vs. TBM) can be done on the basis of identification of 3-hydroxyisovalerate, isobutyrate and formate in case of CSF (with a correct classification of 78 %), alanine in serum (correct classification 60 %), valine and acetone in case of urine (correct classification 89.1 %). The NMR spectral bins based orthogonal signal correction principal component analysis score plots of significant metabolites obtained from DFA also provided group classification among cases versus control group in CSF, serum and urine samples. The variable importance in projection scores also identified similar significant metabolites as obtained from DFA, collectively in CSF, serum and urine samples, responsible for differentiation of meningitis.Conclusion
The CSF contained metabolites which are formed during infection and inflammation, and these were also found in significant quantity in serum and urine samples.56.
57.
Sudhir K. Singh 《Molecular simulation》2016,42(5):413-419
We report the effect of surface-screening parameter of Yukawa potential model on vapour–liquid phase coexistence and critical-point properties of slit–pore-confined Yukawa fluid, using grand canonical transition-matrix Monte Carlo along with the histogram reweighting method. The effect of surface-screening parameter on the vapour–phase coexistence density is insignificant for the studied system. On the other hand, significant effect of surface-screening parameter is observed on liquid phase coexistence density. With increasing surface-screening parameter, liquid phase coexistence density decreases. Critical-point properties have shown monotonic decreasing trends with increase in surface-screening parameter. Moreover, the effect of change of surface-screening parameter is least on critical temperature changes as compared to critical density and critical pressure changes for the studied Yukawa system in this work. 相似文献
58.
Sharma Manisha Sangwan Rajender S. Khatkar Bhupender S. Singh Sudhir P. 《Bioprocess and biosystems engineering》2019,42(10):1681-1693
Bioprocess and Biosystems Engineering - The genes for dextransucrase and dextranase were cloned from the genomic regions of Leuconostoc mesenteroides MTCC 10508 and Streptococcus mutans MTCC 497,... 相似文献
59.
Garsa Anita Kumari Choudhury Prasanta Kumar Puniya Anil Kumar Dhewa Tejpal Malik Ravinder Kumar Tomar Sudhir Kumar 《Probiotics and antimicrobial proteins》2019,11(4):1403-1413
Probiotics and Antimicrobial Proteins - Bovicin is a type AII lantibiotic, possessing two β-methyllanthionine and a disulfide bridge encoded by bovA gene hitherto unknown a couple of decades... 相似文献
60.
Anand Santosh Mandal Surajit Tomar Sudhir Kumar 《Probiotics and antimicrobial proteins》2019,11(1):23-29
Probiotics and Antimicrobial Proteins - The present study was to investigate the utilization of prebiotics by Lactobacillus rhamnosus NCDC 298 and its synergistic adversary effect on both... 相似文献