全文获取类型
收费全文 | 1413篇 |
免费 | 78篇 |
国内免费 | 2篇 |
出版年
2022年 | 20篇 |
2021年 | 29篇 |
2020年 | 19篇 |
2019年 | 14篇 |
2018年 | 35篇 |
2017年 | 29篇 |
2016年 | 47篇 |
2015年 | 65篇 |
2014年 | 70篇 |
2013年 | 86篇 |
2012年 | 109篇 |
2011年 | 94篇 |
2010年 | 68篇 |
2009年 | 46篇 |
2008年 | 65篇 |
2007年 | 43篇 |
2006年 | 49篇 |
2005年 | 53篇 |
2004年 | 36篇 |
2003年 | 51篇 |
2002年 | 33篇 |
2001年 | 36篇 |
2000年 | 34篇 |
1999年 | 29篇 |
1998年 | 21篇 |
1997年 | 12篇 |
1996年 | 8篇 |
1995年 | 14篇 |
1994年 | 7篇 |
1993年 | 7篇 |
1992年 | 27篇 |
1991年 | 22篇 |
1990年 | 19篇 |
1989年 | 20篇 |
1988年 | 17篇 |
1987年 | 23篇 |
1986年 | 17篇 |
1985年 | 14篇 |
1984年 | 8篇 |
1983年 | 10篇 |
1982年 | 11篇 |
1979年 | 5篇 |
1978年 | 9篇 |
1976年 | 7篇 |
1975年 | 6篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 7篇 |
1970年 | 5篇 |
1966年 | 4篇 |
排序方式: 共有1493条查询结果,搜索用时 984 毫秒
191.
María del Rocío Cantero Irina F. Velázquez Andrew J. Streets Albert C. M. Ong Horacio F. Cantiello 《The Journal of biological chemistry》2015,290(39):23888-23896
Polycystin-2 (PC2) is a TRP-type, Ca2+-permeable non-selective cation channel that plays an important role in Ca2+ signaling in renal and non-renal cells. The effect(s) of the cAMP pathway and kinase mediated phosphorylation of PC2 seem to be relevant to PC2 trafficking and its interaction with polycystin-1. However, the role of PC2 phosphorylation in channel function is still poorly defined. Here we reconstituted apical membranes of term human syncytiotrophoblast (hST), containing endogenous PC2 (PC2hst), and in vitro translated channel protein (PC2iv). Addition of the catalytic subunit of PKA increased by 566% the spontaneous PC2hst channel activity in the presence of ATP. Interestingly, 8-Br-cAMP also stimulated spontaneous PC2hst channel activity in the absence of the exogenous kinase. Either stimulation was inhibited by addition of alkaline phosphatase, which in turn, was reversed by the phosphatase inhibitor vanadate. Neither maneuver modified the single channel conductance but instead increased channel mean open time. PKA directly phosphorylated PC2, which increased the mean open time but not the single channel conductance of the channel. PKA phosphorylation did not modify either R742X truncated or S829A-mutant PC2iv channel function. The data indicate that the cAMP pathway regulates PC2-mediated cation transport in the hST. The relevant PKA site for PC2 channel regulation centers on a single residue serine 829, in the carboxyl terminus. 相似文献
192.
193.
194.
195.
Photosynthetic characteristics, specific leaf mass, chlorophyll and total leaf nitrogen concentrations of four herbaceous
plants (Dicranopteris linearis, Hanguana malayana, Pentaphragma ellipticum, Tacca integrifolia) from nutrient-poor tropical
forests showed that all these plants were well-adapted to their natural growth environments. No photoinhibition was observed
even in the understorey plants.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
196.
A method for DNA extraction from Pyrrosia piloselloides (L.) Price and Pyrrosia longifolia (Burm.) Morton utilising hexadecyltrimethylammonium
bromide is described. The genomic DNA isolated was found to be relatively pure and shown to be suitable for RAPD analysis.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
197.
Applied Microbiology and Biotechnology - The aim of this work was to study the solid waste (manure) produced by catfish as a potential feedstock for the production of lactic acid (LA) via... 相似文献
198.
Huihua Sun Hongfang Zhang Ee Lui Ang Huimin Zhao 《Bioorganic & medicinal chemistry》2018,26(7):1275-1284
Biocatalysis has been increasingly used for pharmaceutical synthesis in an effort to make manufacturing processes greener and more sustainable. Biocatalysts that possess excellent activity, specificity, thermostability and solvent-tolerance are highly sought after to meet the requirements of practical applications. Generating biocatalysts with these specific properties can be achieved by either discovery of novel biocatalysts or protein engineering. Meanwhile, chemoenzymatic routes have also been designed and developed for pharmaceutical synthesis on an industrial scale. This review discusses the recent discoveries, engineering, and applications of biocatalysts for the synthesis of pharmaceuticals and pharmaceutical intermediates. Key classes of biocatalysts include reductases, oxidases, hydrolases, lyases, isomerases, and transaminases. 相似文献
199.
Shuobo Shi Ee Lui Ang Huimin Zhao 《Journal of industrial microbiology & biotechnology》2018,45(7):491-516
In vivo biosensors can recognize and respond to specific cellular stimuli. In recent years, biosensors have been increasingly used in metabolic engineering and synthetic biology, because they can be implemented in synthetic circuits to control the expression of reporter genes in response to specific cellular stimuli, such as a certain metabolite or a change in pH. There are many types of natural sensing devices, which can be generally divided into two main categories: protein-based and nucleic acid-based. Both can be obtained either by directly mining from natural genetic components or by engineering the existing genetic components for novel specificity or improved characteristics. A wide range of new technologies have enabled rapid engineering and discovery of new biosensors, which are paving the way for a new era of biotechnological progress. Here, we review recent advances in the design, optimization, and applications of in vivo biosensors in the field of metabolic engineering and synthetic biology. 相似文献
200.
Karpe A. V. Dunn M. S. Taylor M. C. Nguyen T. Ong C. Karla T. Rockman S. Beale D. J. 《Metabolomics : Official journal of the Metabolomic Society》2018,14(12):1-1
Metabolomics - The identification of metabolomic dysregulation appears promising for the prediction of type 1 diabetes and may also reveal metabolic pathways leading to beta-cell destruction.... 相似文献