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151.
J. -K. Huang K. C. Keudell Su-Jin. Seong W. E. Klopfenstein L. Wen M. O. Bagby R. A. Norton R. F. Vesonder 《Biotechnology letters》1996,18(2):193-198
Summary A new microbial isolate, Bacillus sp. U88, transformed 12-hydroxyoctadecanoic acid to 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids when grown aerobically in 1 % yeast extract medium at 30°C and shaken at 250 rpm for 5 days. These three dihydroxyfatty acids were derivatized with Trimethylsilyl ether and identified by GC-MS analysis. The yield of 12,15-, 12,16- and 12,17-dihydroxyoctadecanoic acids after 5 day incubation were 2.8 %, 7.1 % and 1.1 %, respectively.Names are necessary to report factually on available data; however the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of other that may also be suitable. 相似文献
152.
153.
Supercapacitors: Large‐Scale Conductive Yarns Based on Twistable Korean Traditional Paper (Hanji) for Supercapacitor Applications: Toward High‐Performance Paper Supercapacitors (Adv. Energy Mater. 27/2018) 下载免费PDF全文
154.
155.
Cheung-Kue Kim Sunju Oh Sook-Ja Kim Sun-Hee Leem Jeonghoon Heo So-Hak Chung 《Genes & genomics.》2018,40(4):381-388
Osteosarcoma is the most common type of malignant bone tumors. Insulin Growth Factor 1 receptor (IGFR1) has been known as a prognostic factor for metastasis of osteosarcoma. ABC subfamily G member2 (ABCG2) is related to resistance to anti-cancer drug, and CD44 has a role in tumor growth and metastasis. The purpose of this study is to investigate the relationship among expression patterns of IGF1R, ABCG2, and CD44 in osteosarcoma. The expression levels of IGF1R, ABCG2, and CD44 proteins were determined in tissue arrays containing osteosarcoma tissues from 59 osteosarcoma patients. The expression pattern of IGF1R was highly correlated with the expression pattern of ABCG2 (r?=?0.88) in overall osteosarcoma patients. According to pathological types, the expression pattern of IGF1R showed the higher correlation with ABGC2 (r?=?0.90) and CD44 (r?=?0.61) in osteoblatic type than in chondroblastic type. According to gender with pathologic type, the correlation between the expression patterns of IGF1R and CD44 was higher in male with osteoblatic type than in female with osteoblatic type. Among different age groups, the 1–10 years age group showed higher correlation in IGF1R versus CD44 (r?=?0.90) and ABCG2 versus CD44 (0.80) than in other age groups. These results showed that the expression of IGF1R appears to be highly correlated with the expression of ABCG2 in osteosarcoma and with the expression of CD44 in osteosarcoma patients under age of 10, which suggests that ABCG2 and CD44 can be used as prognostic factors with IGF1R for specific prognosis and efficient treatment of osteosarcoma. 相似文献
156.
Nayoung Heo 《Ethnic and racial studies》2018,41(15):2750-2769
The present study explores the ethnic and religious identities of 21 multicultural young adults with a Japanese mother and a Korean father, all residing in South Korea where the “myth” of a mono-ethnic nation still lingers. In the study, one is considered multicultural provided they are born to both one foreign-born parent and one Korean-born parent. All interviewees are members of the Family Federation for World Peace Unification religion established in South Korea in the 1950s. Interviews with these young adults showed (a) that there was an intersectionality between their ethnic identity and religious identity; (b) that the influence of the interaction between the Church and the mother on the ethnic and religious identities of the interviewees was substantial; and (c) that they distinguished themselves from other multicultural individuals and even from non-multicultural individuals concerning their socio-economic background and transcending religious mission. 相似文献
157.
Inja Cho Won-Joo Kim Hyun-Woo Kim Kyoung Heo Byung In Lee Yang-Je Cho 《Neurochemical research》2018,43(7):1464-1475
Excessive production of reactive oxygen species (ROS), along with dysfunction of the antioxidant defense system, such as that involving superoxide dismutase (SOD), may play a major role in neuronal death following status epilepticus (SE). Neurosteroids, which are allosteric modulators of the GABAA receptor in cerebral metabolism, have been suggested as being neuroprotective in various animal models; however, their effect to preventing ROS has not been examined. Herein, we investigate the neuroprotective role of allopregnanolone, the prototypical neurosteroid in the brain, in relation to the ROS-mediated neuronal injury. Adult male C57BL/6 mice were subjected to SE and treated with allopregnanolone. Hippocampal cell death was assessed by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and ROS production was investigated by in situ detection of oxidized hydroethidine. SOD2 expression was analyzed by both western blot and immunofluorescent staining in the hippocampal subfields. In mice treated with allopregnanolone after SE, hippocampal cell death, DNA fragmentation, oxidative DNA damage, and ROS production were reduced significantly compared to mice subjected to vehicle treatment after SE. Hippocampal SOD2 expression was significantly increased by allopregnanolone. These finding suggest that allopregnanolone plays a neuroprotective role, with not only anticonvulsant but also antioxidant effects, by increasing SOD2 in pilocarpine-induced SE model. 相似文献
158.
Although magnetic resonance imaging (MRI) can accurately measure lower limb skeletal muscle (SM) mass, this method is complex and costly. A potential practical alternative is to estimate lower limb SM with dual-energy X-ray absorptiometry (DXA). The aim of the present study was to develop and validate DXA-SM prediction equations. Identical landmarks (i.e., inferior border of the ischial tuberosity) were selected for separating lower limb from trunk. Lower limb SM was measured by MRI, and lower limb fat-free soft tissue was measured by DXA. A total of 207 adults (104 men and 103 women) were evaluated [age 43 +/- 16 (SD) yr, body mass index (BMI) 24.6 +/- 3.7 kg/m(2)]. Strong correlations were observed between lower limb SM and lower limb fat-free soft tissue (R(2) = 0.89, P < 0.001); age and BMI were small but significant SM predictor variables. In the cross-validation sample, the differences between MRI-measured and DXA-predicted SM mass were small (-0.006 +/- 1.07 and -0.016 +/- 1.05 kg) for two different proposed prediction equations, one with fat-free soft tissue and the other with added age and BMI as predictor variables. DXA-measured lower limb fat-free soft tissue, along with other easily acquired measures, can be used to reliably predict lower limb skeletal muscle mass. 相似文献
159.
The Saccharomyces cerevisiae gene RHC21 is a homologue of the fission yeast rad21
+gene, which affects the sensitivity of cells to γ-irradiation and is essential for cell growth in S. pombe. Disruption of the RHC21 gene showed that it is also essential in S. cerevisiae. To examine its function in cell growth further, we have isolated temperature-sensitive mutants for the RHC21 gene and characterized one of them, termed rhc21-sk16. When this mutant was incubated at 36° C, the percentage of large-budded cells was increased. Most of the large-budded cells
had aberrant nuclear structures, such as unequally extended nuclear DNA with incompletely elongated spindles across the mother-daughter
neck or only in a mother cell. Furthermore, a circular minichromosome is more unstable in the mutant than in the wild-type,
even at 25° C. Flow cytometry showed that the bulk of DNA replication takes place normally at the restrictive temperature
in the mutant. These results indicated that the RHC21 gene is required for proper segregation of the chromosomes. In addition, we found that the mutant is sensitive not only to
UV radiation and γ-rays but also to the antimicrotubule agent nocodazole at 25° C. This suggests that the RHC21 gene is involved in the microtubule function. We discuss how the RHC21 gene product may be involved in chromosome segregation and microtubule function.
Received: 10 March 1997 / Accepted: 1 September 1997 相似文献
160.
Sojeong Lee Mi Gyeom Kim Nury Kim Won Do Heo Gyun Min Lee 《Biotechnology and Bioprocess Engineering》2016,21(3):439-445
Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the most popular host cells for transient gene expression (TGE) of therapeutic proteins. These host cells require high transfection efficiency in order to enhance TGE. Heparan sulfate proteoglycan (HSPG) at the cell surface is known to regulate endocytosis for gene delivery. The HSPG expression in CHO DG44 and HEK293E cells was investigated in an effort to enhance the TGE. Immunostaining of HSPGs followed by confocal microscopy and flow cytometry analyses revealed that CHO DG44 cells possessed a higher amount of cell-surface and intracellular HSPGs than HEK293E cells. The mRNA levels of the representative enzymes involved in the HSPG biosynthesis in CHO DG44, which were determined by quantitative real time PCR, were quite different from those in HEK293E cells. Taken together, the results obtained here would be useful in improving TGE in CHO DG44 and HEK293E cells through genetic engineering of HSPG synthesis. 相似文献