全文获取类型
收费全文 | 64篇 |
免费 | 5篇 |
出版年
2021年 | 1篇 |
2020年 | 1篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 5篇 |
2013年 | 6篇 |
2012年 | 1篇 |
2011年 | 3篇 |
2010年 | 5篇 |
2009年 | 4篇 |
2008年 | 2篇 |
2007年 | 3篇 |
2006年 | 3篇 |
2005年 | 1篇 |
2004年 | 4篇 |
2002年 | 2篇 |
2001年 | 1篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1977年 | 1篇 |
排序方式: 共有69条查询结果,搜索用时 31 毫秒
41.
Clint A. James Patrick DeRoy Martin Duplessis Paul J. Edwards Teddy Halmos Joannie Minville Louis Morency Sébastien Morin Bruno Simoneau Martin Tremblay Richard Bethell Michael Cordingley Jianmin Duan Louie Lamorte Alex Pelletier Daniel Rajotte Patrick Salois Sonia Tremblay Claudio F. Sturino 《Bioorganic & medicinal chemistry letters》2013,23(9):2781-2786
42.
Marcelo L Laia Leandro M Moreira Juliana Dezajacomo Joice B Brigati Cristiano B Ferreira Maria IT Ferro Ana CR Silva Jesus A Ferro Julio CF Oliveira 《BMC microbiology》2009,9(1):12-17
Background
Citrus canker is a disease caused by the phytopathogens Xanthomonas citri subsp. citri, Xanthomonas fuscans subsp. aurantifolli and Xanthomonas alfalfae subsp. citrumelonis. The first of the three species, which causes citrus bacterial canker type A, is the most widely spread and severe, attacking all citrus species. In Brazil, this species is the most important, being found in practically all areas where citrus canker has been detected. Like most phytobacterioses, there is no efficient way to control citrus canker. Considering the importance of the disease worldwide, investigation is needed to accurately detect which genes are related to the pathogen-host adaptation process and which are associated with pathogenesis. 相似文献43.
Background
Phylogenetic analyses of the Annonaceae consistently identify four clades: a basal clade consisting of Anaxagorea, and a small 'ambavioid' clade that is sister to two main clades, the 'long branch clade' (LBC) and 'short branch clade' (SBC). Divergence times in the family have previously been estimated using non-parametric rate smoothing (NPRS) and penalized likelihood (PL). Here we use an uncorrelated lognormal (UCLD) relaxed molecular clock in BEAST to estimate diversification times of the main clades within the family with a focus on the Asian genus Pseuduvaria within the SBC. Two fossil calibration points are applied, including the first use of the recently discovered Annonaceae fossil Futabanthus. The taxonomy and morphology of Pseuduvaria have been well documented, although no previous dating or biogeographical studies have been undertaken. Ancestral areas at internal nodes within Pseuduvaria are determined using dispersal-vicariance analysis (DIVA) and weighted ancestral area analysis (WAAA). 相似文献44.
Vincenzo Trotta Federico CF Calboli Marcello Ziosi Daniela Guerra Maria C Pezzoli Jean R David Sandro Cavicchi 《BMC evolutionary biology》2006,6(1):67
Background
Populations of Drosophila melanogaster show differences in many morphometrical traits according to their geographic origin. Despite the widespread occurrence of these differences in more than one Drosophila species, the actual selective mechanisms controlling the genetic basis of such variation are not fully understood. Thermal selection is considered to be the most likely cause explaining these differences. 相似文献45.
Antisense RNA Targeting of Primase Interferes with Bacteriophage Replication in Streptococcus thermophilus
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The putative primase gene and other genes associated with the Sfi21-prototype genome replication module are highly conserved in Streptococcus thermophilus bacteriophages. Expression of antisense RNAs complementary to the putative primase gene (pri3.1) from S. thermophilus phage κ3 provided significant protection from κ3 and two other Sfi21-type phages. Expression of pri3.10-AS, an antisense RNA that covered the entire primase gene, reduced the efficiency of plaquing (EOP) of κ3 to 3 × 10−3 and reduced its burst size by 20%. Mutant phages capable of overcoming antisense inhibition were not recovered. Thirteen primase-specific antisense cassettes of different lengths (478 to 1,512 bp) were systematically designed to target various regions of the gene. Each cassette conferred some effect, reducing the EOP to between 0.8 and 3 × 10−3. The largest antisense RNAs (1.5 kb) were generally found to confer the greatest reductions in EOP, but shorter (0.5 kb) antisense RNAs were also effective, especially when directed to the 5′ region of the gene. The impacts of primase-targeted antisense RNAs on phage development were examined. The expression of pri3.10-AS resulted in reductions in target RNA abundance and the number of phage genomes synthesized. Targeting a key genome replication function with antisense RNA provided effective phage protection in S. thermophilus. 相似文献
46.
47.
Juteau H Gareau Y Labelle M Sturino CF Sawyer N Tremblay N Lamontagne S Carrière MC Denis D Metters KM 《Bioorganic & medicinal chemistry》2001,9(8):1977-1984
Potent and selective antagonists of the human EP3 receptor have been identified. The structure-activity relationship of the chemical series was conducted and we found several analogues displaying sub-nanomolar K(i) values at the EP3 receptor and micromolar activities at the EP1, EP2 and EP4 receptors. The effect of added human serum albumin (HSA) on the binding affinity at the EP3 receptor was also investigated. 相似文献
48.
Rates of DNA sequence evolution are not sex-biased in Drosophila melanogaster and D. simulans 总被引:2,自引:0,他引:2
To determine whether male- or female-biased mutation rates have affected
the molecular evolution of Drosophila melanogaster and D. simulans, we
calculated the male-to-female ratio of germline cell divisions ([symbol:
see text]) from germline generation data and the male-to-female ratio of
mutation rate ([symbol: see text]) by comparing chromosomal levels of
nucleotide divergence. We found that the ratio of germline cell divisions
changes from indicating a weak female bias to indicating a weak male bias
as the age of reproduction increases. The range of [symbol: see text]
values that we observed, however, does not lead us to expect much, if any,
difference in mutation rate between the sexes. Silent and intron nucleotide
divergence were compared between nine loci on the X chromosome and nine
loci on the second and third chromosomes. The average levels of nucleotide
divergence were not significantly different across the chromosomes,
although both silent and intron sites show a trend toward slightly more
divergence on the X. These results indicate a lack of sex- or
chromosome-biased molecular evolution in D. melanogaster and D. simulans.
相似文献
49.
50.