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141.
Christian Berg Hans Dieter Knapp Ulrich Messner Wolfgang Wiehle 《Folia Geobotanica》1989,24(3):297-304
(Bellevalia ciliata was recorded in north-east Bulgaria south of the Dobrudsha, within field and steppe vegetation. Vegetation records and a distribution map are presented. Based on taxonomic studies it is proposed to combineB. ciliata, B. sarmatica (Pall.) Wor. andB. speciosa Wor. under the oldest nameB. ciliata (Cyr.) Nees. 相似文献
142.
Sandra Youngman Mansoor Sarfarazi Maja Bucan Marcy MacDonald Barbara Smith Michael Zimmer Conrad Gilliam Anna-Maria Frischauf John J. Wasmuth James F. Gusella Hans Lehrach Peter S. Harper Duncan J. Shaw 《Genomics》1989,5(4)
Genetic linkage studies have mapped Huntington's disease (HD) to the distal portion of the short arm of chromosome 4 (4p16.3), 4 cM distal to D4S10 (G8). To date, no definite flanking marker has been identified. A new DNA marker, D4S90 (D5), which maps to the distal region of 4p16.3, is described. The marker was used in a genetic linkage study in the CEPH reference families with seven other markers at 4p16. The study, together with knowledge of the physical map of the region, places D4S90 as the most distal marker, 6 cM from D4S10. A provisional linkage study with HD gave a maximum lod score of 2.14 at a θ of 0.00 and no evidence of linkage disequilibrium. As D4S90 appears to be located terminally, it should play an important role in the accurate mapping and cloning of the HD gene. 相似文献
143.
Fusion of cultured dog kidney (MDCK) cells: I. Technique,fate of plasma membranes and of cell nuclei
Ulrich Kersting Heribert Joha Wieland Steigner Birgit Gassner Gerhard Gstraunthaler Walter Pfaller Hans Oberleithner 《The Journal of membrane biology》1989,111(1):37-48
Summary The evaluation of the intracellular signal train and its regulatory function in controlling transepithelial transport with electrophysiological methods often requires intracellular measurements with microelectrodes. However, multiple impalements in epithelial cells are hampered by the small size of the cells. In an attempt to avoid these problems we fused cells of an established cell line, Madin Darby canine kidney cells, originally derived from dog kidney, to giant cells by applying a modified polyethylene glycol method. During trypsin-induced detachment from the ground of the petri dish, individual cells grown in a monolayer incorporate volume and mainly lose basolateral plasma membrane by extrusion. By isovolumetric cell-to-cell fusion, spherical giant cells are formed within 2 hr. During this process a major part of the individual cell plasma membranes is internalized. Over three weeks following cell plasma membrane fusion degradation of single cell nuclei and cell nuclear fusion occurs. We conclude that this experimental approach opens the possibility to investigate ion transport of epithelia in culture by somatic cell genetic techniques. 相似文献
144.
145.
Carl Harald Janson Mahmood Jeddi Tehrani H»kan Mellstedt Hans Wigzell 《Cancer immunology, immunotherapy : CII》1989,28(3):225-232
Summary A murine anti-idiotypic monoclonal antibody (mAb), F1, (IgG2a) was produced against the variable part of the T-cell receptor for antigen (Ti, /) on the tumor cells of a patient with T-cell chronic lymphatic leukemia (CD3+, 8+, 4–). The molecular weight of the protein reactive with mAb F1, comodulation and coprecipitation with anti-CD3 antibody, and the restricted tumor-cell reactivity strongly support the anti-idiotypic nature of mAb F1. MAb F1 also stained 4% of peripheral blood lymphocytes of healthy donors. MAb F1 did not stimulate the tumor cells to DNA synthesis, but stimulated a fraction of the normal peripheral blood lymphocytes, mAb F1 did not mediate antibody-dependent cellular cytotoxicity or complement lysis to any significant degree in vitro. Three infusions of 1–10 mg anti-idiotypic mAb were given over a period of 4 weeks. The plasma half-life for mAb F1 was 3 h in the first 2 h after infusion and 44 h from 2 h to 120 h after infusion. After each treatment a rapid decrease of circulating tumor cells was seen. During the observation period an 80% reduction of the total circulating tumor cells was noted. After the second infusion, IgM and IgG antimouse antibodies were detected. Side-effects from therapy were fever, chills, nausea, vomiting, diarrhea, tachycardia, increase in systolic blood pressure and shortness of breath. Thus, in T-cell malignancies a major reduction of circulating tumor cells can be accomplished by low doses of anti-idiotypic mAb. Anti-idiotypic mAb might be a therapeutic agent of significant importance. 相似文献
146.
Hans -Georg Heinzel Hartmut Böhm 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1989,164(6):775-786
The wind-orientation of carrion beetles (Necrophorus humator F.) was studied by use of a locomotion-compensator.
相似文献
1. | Beetles walking on a horizontal surface for periods of several minutes in a dark environment without an air current and other orientational stimuli seldom keep straight paths. They walk along individually different circular paths (Fig. 1). The mean walking speed is 5.6±1.0 cm/s. The mean of the angular velocity reaches maximally 25 °/s for individual beetles (mean angular velocity of the analysed population of 152 beetles: 1.9±9.3 °/s). The distribution of the mean walking directions of the population shows that the beetles display no preference for one direction (Fig. 3 A). The instantaneous value of the individual angular velocity is independent of the instantaneous walking direction. |
2. | During exposure to an air current the individual beetles keep straight and stable courses with any orientation relative to the direction of air flow (Fig. 4). The mean walking directions of 76 individuals point in all directions but there is a weak preference of windward tracks (Fig. 3B). |
3. | Wind orientated walking starts at a threshold wind velocity of about 5 cm/s (Fig. 6). The walking tracks straighten with increasing air current velocity. This leads to a narrowing of the distribution of the instantaneous walking directions around the preferred walking direction (Fig. 7C). This narrowing is due to an increase in the slope of the characteristic curve (angular velocity as a function of walking direction) of the wind-orientation system. |
4. | Twenty percent of the beetles show a spontaneous change of their anemotactic course during walks of 5 min duration. Neither the time of the change, its position on the track or the direction of the new course are predictable. There is, however, a slight preference for 90±20° changes in the walking direction (Fig. 8). |
5. | The antennae (Fig. 9) act as the only sense organs responsible for the wind orientation. The capability for wind orientated walks is lost after ablation of both flagella (Fig. 10). |
147.
Hans -Ortwin Nalbach Jochen Zeil Luise Forzin 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1989,165(5):643-649
1. | We recorded compensatory eye stalk movements in response to pitch and roll stimulation of the visual, statocyst, and leg-proprioceptive systems in different species of crabs (Carcinus maenas, Heloecius cordiformis, Pachygrapsus marmoratus) (Fig. 2). |
2. | The relative contribution of visual, statocyst and leg-proprioceptive inputs to eye stabilization in space varies greatly among different species (Fig. 3). |
3. | We suggest that for stabilizing the eyes in space, the contribution of various sensory inputs in different species of crabs correspond to the availability of cues in their habitat. Semiterrestrial crabs living in a habitat with well defined and predictable visual geometry stabilize their eye stalks mainly by visual cues. Crabs living on solid substrate make strong use of leg proprioceptive input. Swimming crabs, and other predominantly aquatic crabs, rely mainly on their statocysts. |
148.
Hans R. Preisig 《Plant Systematics and Evolution》1989,164(1-4):209-214
A new species ofMallomonas, M. alphaphora (Chrysophyceae), was found in freshwater ponds in the Perth region, Western Australia. It is distinguished from other species ofMallomonas by its very distinctive scale and bristle morphology and is placed in a new section,Alphaphorae, of the genusMallomonas.
Dedicated to Prof. DrL. Geitler on the occasion of the 90th anniversary of his birthday. 相似文献
149.
150.
Cysteine: Depolarization-Induced Release from Rat Brain In Vitro 总被引:3,自引:2,他引:1
Hans Jörg Keller Kim Quang Do Markus Zollinger Kaspar H. Winterhalter Michel Cuénod 《Journal of neurochemistry》1989,52(6):1801-1806
Compounds released on depolarization in a Ca2+-dependent manner from rat brain slices were screened to identify candidates for neuroactive substances. Lyophilized superfusates were analyzed by reversed-phase HPLC after derivatization with 9-fluorenyl N-succinimidyl carbonate. One of the compounds that showed an increase of concentration in superfusates in the presence of iodoacetamide was identified as the cysteine (Cys) derivative, S-carboxamidomethylcysteine, by fast atom bombardment mass spectrometry and other methods. This stable Cys derivative originates from endogenous, extracellular Cys. The finding led to a method for quantification of Cys in superfusates by immediate cooling of the superfusates to 0 degrees C and reaction of Cys with N-ethylmaleimide. Depolarization-induced Ca2+-dependent release of Cys was most prominent in the neocortex, followed by the mesodiencephalon, striatum, and cerebellum. This suggests that Cys is released from a neuronal compartment and might be involved in neurotransmission. 相似文献