排序方式: 共有62条查询结果,搜索用时 15 毫秒
21.
22.
We analysed complexes formed during recognition of the lacUV5 promoter by E. coli RNA polymerase using formaldehyde as a DNA-protein and protein-protein cross-linking reagent. Most of the cross-linked complexes specific for the open complex (RPO) contain the beta' subunit of RNA polymerase cross-linked with promoter DNA in the regions: -50 to -49; -5 to -10; + 5 to +8 and +18 to +21. The protein-protein cross-linking pattern of contacting subunits is the same for the RNA polymerase in solution and in RPO: there are strong sigma-beta' and beta-beta' interactions. In contrast, only beta-beta' cross-links were detected in the closed (RPC) and intermediate (RPI) complexes. In presence of lac repressor before or after formation of the RPO cross-linking pattern is similar with that of RPI (RPC) complex. 相似文献
23.
24.
25.
26.
V. M. Studitsky 《Molecular Biology》2009,43(2):188-197
Enhancers and insulators are regulatory DNA sequences that can work over a large distance. Efficient action over a distance
clearly requires special mechanisms for facilitating communication between a regulatory region and its target. Studies from
our laboratory identified DNA supercoiling as primary factor that mediates efficient enhancer-promoter communication over
a distance in prokaryotes through a “DNA slithering” mechanism. These studies allowed rational design and construction of
an insulator that can block enhancer action over a distance both in vitro and in vivo. Our most recent studies suggest that
eukaryotic chromatin structure can support action over a distance using similar principles, but in a mechanistically distinct
way.
Published in Russian in Molekulyarnaya Biologiya, 2009, Vol. 43, No. 2, pp. 204–214.
The article was translated by the author. 相似文献
27.
28.
29.
30.