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51.
R S Reid A L Stuart S V Gupta L J Latimer B L Haug J S Lee 《Nucleic acids research》1987,15(10):4325-4335
Synthetic DNAs were prepared containing 6-methyl adenine (m6A) in place of adenine and 5-ethyl uracil (Et5U) or 5-methoxymethyl uracil (Mm5U) in place of thymine. All three modifications destabilized duplex DNAs to varying degrees. The binding of ethidium was studied to analogues of poly[d(AT)]. There was no evidence of cooperative binding and the "neighbour exclusion rule" was obeyed in all cases although the binding constant to poly[d(m6AT)] was approximately 6 fold higher than to poly[d(AT)]. 31P NMR spectra were recorded in increasing concentrations of CsF. Poly[d(AEt5U)] showed two well-resolved signals separated by 0.55 ppm in 1 M CsF compared to 0.32 ppm for poly[d(AT)] under identical conditions. In contrast, poly[d(AMm5U)] and poly[d(m6AT)] showed two signals separated by 0.28 ppm and 0.15 ppm respectively, only when the concentration of CsF was raised to 2 M. The signals for poly[d(AT)] in 2 M CsF were better resolved and were separated by 0.41 ppm. These results suggest that minor modifications to the bases may have conformational effects which could be recognized by DNA-binding proteins. 相似文献
52.
Anaerobic respiration in the Rhodospirillaceae: characterisation of pathways and evaluation of roles in redox balancing during photosynthesis 总被引:2,自引:0,他引:2
Abstract Recent discoveries relating to pathways of anaerobic electron transport in the Rhodospirillaceae are reviewed. The main emphasis is on the organism Rhodobacter capsulatus ** but comparisons are made with Rhodobacter sphaeroides ** f. sp. denitrificans and Rhodopseudomonas palustris . The known electron acceptors for anaerobic respiration in Rhodobacter capsulatus are trimethylamine- N -oxide (TMAO), dimethyl sulphoxide (DMSO), nitrate and nitrous oxide. In each case respiration generates a proton electrochemical gradient and in some cases can support growth on non-fermentable carbon sources. However, the principal objective of this review is to discuss the possibility that, apart from a role in energy conservation, anaerobic respiration in the photosynthetic bacteria may have a special function in maintaining redox balance during photosynthetic metabolism. Thus the electron acceptors mentioned above may serve as auxiliary oxidants: (a) to maintain an optimal redox poise of the photosynthetic electron transport chain; (b) to provide a sink for electrons during phototrophic growth on highly reduced carbon substrates.
Molecular properties of the nitrate reductase, nitrous oxide reductase and a single enzyme responsible for reduction of TMAO and DMSO are discussed. These enzymes are all located in the periplasm. Electrons destined for all three enzymes can originate from the rotenone-sensitive NADH dehydrogenase but do not proceed through the antimycin- and myxothiazol-sensitive cytochrome b/c1 complex. It is likely, therefor, that the pathways of anaerobic respiration overlap with the cyclic photosynthetic electron transport chain only at the level of the ubiquinone pool. Redox components which might be involved in the terminal branches of anaerobic respiration are discussed. 相似文献
Molecular properties of the nitrate reductase, nitrous oxide reductase and a single enzyme responsible for reduction of TMAO and DMSO are discussed. These enzymes are all located in the periplasm. Electrons destined for all three enzymes can originate from the rotenone-sensitive NADH dehydrogenase but do not proceed through the antimycin- and myxothiazol-sensitive cytochrome b/c
53.
Crossovers in two German cystic fibrosis families determine probe order for MET, 7C22 and XV-2c/CS.7 总被引:6,自引:0,他引:6
W. Berger J. Hein J. Gedschold I. Bauer A. Speer M. Farrall R. Williamson C. Coutelle 《Human genetics》1987,77(2):197-199
Summary We have followed the segregation of the probes pJ3.11, 7C22, pB79a, and MET through cystic fibrosis families in the German Democratic Republic with two affected sibs. Two families with a crossover between MET and the CF phenotype were detected. In one of these families recombination was also observed between the DNA probe 7C22 and CF, and between the markers XV-2c and CF, which suggests that XV-2c, MET and 7C22 are all on the same side of CF. The other MET recombinant family is informative with XV-2c and does not recombine, which excludes the genetic order XC-2c-MET-CF if multiple recombinant events are disregarded. These two families together demonstrate that recombinations may occur in a very small genetic interval, which has important implications for prenatal diagnosis based on data from linked markers. 相似文献
54.
Summary The human progesterone receptor gene was mapped by in situ hybridization using two cDNA probes corresponding to the 5′ and
3′ part of the coding sequence. This gene was localized to 11q22-q23. 相似文献
55.
Summary Specimens of the terrestrial gastropod Arianta arbustorum were fed on cadmium- or copper-enriched agar plates with the aim of performing an input/output analysis and of studying the distribution of these metals in several organs of the snails. After a feeding period of 20 days about 45% of cadmium were lost. 36% accumulated in the hepatopancreas, where a cadmium concentration of more than 500 g/g was measured. The efficiency of cadmium assimilation decreased from about 90% at the beginning to about 55% after 20 days. Copper was distributed more evenly than cadmium, but the main site of copper storage seemed to be the foot/mantle tissues, where 49% of the ingested copper were found. The efficiency of copper assimilation always exceeded 95%. The patterns of distribution and assimilation of copper and cadmium are discussed in relation to differences in the cytological and biochemical detoxification mechanisms which exist for these metals in molluscs. 相似文献
56.
Berger TG 《The Western journal of medicine》1989,151(6):649
The Scientific Board of the California Medical Association presents the following inventory of items of progress in dermatology. Each item, in the judgment of a panel of knowledgeable physicians, has recently become reasonably firmly established both as to scientific fact and important clinical significance. The items are presented in simple epitome and an authoritative reference, both to the item itself and to the subject as a whole, is generally given for those who may be unfamiliar with a particular item. The purpose is to assist busy practitioners, students, research workers, or scholars to stay abreast of these items of progress in dermatology that have recently achieved a substantial degree of authoritative acceptance, whether in their own field of special interest or another.The items of progress listed below were selected by the Advisory Panel to the Section on Dermatology of the California Medical Association, and the summaries were prepared under its direction. 相似文献
57.
Newly recognized ectrodactyly/deafness syndrome 总被引:1,自引:0,他引:1
A Raas-Rothschild A Aviram T Ben-Ami I Berger M B Katznelson R M Goodman 《Journal of craniofacial genetics and developmental biology》1989,9(2):121-127
A 7-year-old non-Ashkenazi Jewish girl is described having asymmetrical ectrodactyly (split hand and foot deformity), short stature, mental retardation, sensorineural deafness, and abnormal facies. Because this constellation of findings has not been reported previously, the authors believe that this represents a new congenital malformation syndrome, most probably of genetic etiology. 相似文献
58.
Stuart K 《Parasitology today (Personal ed.)》1989,5(1):5-8
59.
Reclustering of scattered Golgi elements occurs along microtubules 总被引:43,自引:0,他引:43
W C Ho V J Allan G van Meer E G Berger T E Kreis 《European journal of cell biology》1989,48(2):250-263
Depolymerization of the interphase microtubules by nocodazole results in the scattering and apparent fragmentation of the Golgi apparatus in Vero fibroblast cells. Upon removal of the drug, the interphase microtubules repolymerize, and the scattered Golgi elements move back to the region around the microtubule-organizing center (MTOC) within 40 to 60 min. Using a fluorescent lipid analogue (C6-NBD-ceramide) as a vital stain for the scattered Golgi elements, their relocation was visualized by video-enhanced fluorescence microscopy in Vero cells maintained at 20 degrees C. The NBD-labeled structures were identified as Golgi elements by their colocalization with galactosyltransferase in the fixed cells. During reclustering, NBD-labeled Golgi elements were observed to move by discontinuous saltations towards the MTOC with velocities of 0.1 to 0.4 micron/s. Paths along which Golgi elements moved were super-imposable on microtubules visualized by indirect immunofluorescence. Neither the collapse of intermediate filaments caused by microinjection of antibodies to vimentin nor the disruption of microfilaments by cytochalasin D had an effect on the reclustering of Golgi elements or the positioning of the Golgi apparatus. These data show that scattered Golgi elements move along microtubules back to the region around the MTOC, while neither intact intermediate filaments nor microfilaments are involved. 相似文献
60.
Amelie Berger 《Entomologia Experimentalis et Applicata》1989,50(3):199-207
The egg laying pattern of the spotted stalk borer on sorghum and maize, Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae) was studied during the first three nights of oviposition under laboratory conditions. More than 50% of both eggs and egg batches are laid during the first night of oviposition. Batch size decreases with time, whereas mean egg weight remains constant.Larger females lay both larger eggs and egg batches than smaller ones. They are also more fecund but it is only the second night of oviposition that contributes significantly to this difference.The reproductive effort decreases much with time. Allocation of reproductive reserves to early eggs seems more important than having eggs evenly distributed over time. Larger females use proportionally more resources to the production of late eggs. Large eggs are likely to contain more yolk which could be of importance for the ballooning behaviour of newly hatched larvae.
Résumé La ponte de C. partellus Swinhoe a été étudiée au laboratoire pendant les trois premières nuits de la ponte. Plus de 50% des ooplaques et des oefs ont été pondus pendant la première nuit. La taille des ooplaques diminue avec le temps, tandis que le poids moyen des oeufs reste constant.Les grosses femelles pondent des ooplaques et des oeufs plus grands que les petites. Elles sont aussi plus fécondes, mais c'est seulement à la seconde nuit de ponte qu'est due cette différence.Le reproduction diminue beaucoup avec le temps. L'affectation aux premiers oeufs des réserves utilizées pour la reprodution semble plus importante que l'émission d'oeufs régulièrement répartis dans le temps. Les grosses femelles utilisent proportionnellement plus de ressources à la production d'oeufstardifs. Les gros oeufs contiennent vraisemblablement plus de vitellus que pourrait être nécessaire pour le comportement aérostatique des chenilles néonates.相似文献