Mathematical approach to modeling of the treatment of suppurative processes

Consideration of an inflammation focus as an "open system" provided analogy between microbiological processes in inflamed wounds and in systems of continuous cultivation of microorganisms. Mathematical modeling of such systems is widely used. Some of the methods for the mathematical modeling were applied to chemoprophylaxis and chemotherapy of postoperative wounds. In modeling continuous cultivation of microorganisms it is usually necessary to determine optimal conditions for the maximum yield of their biomass. In modeling of wound treatment the aim was to determine the process parameters providing the minimum biomass. The described simple models showed that there could be certain optimal flow rate of the washing fluid in the aspiration-washing procedure for wound treatment at which the drug was not completely washed out while the growth rate of the microbial population was minimal. Such mathematical models were shown valuable in optimizing the use of bactericidal and bacteriostatic antibiotics.     

Therapeutic effect of purified components of a turtle spleen extract

The administration of a spleen extract of Testudo horsfieldi to mice exposed to lethal radiation of 8 Gy was shown to increase the survival rate and to stimulate haemopoiesis and formation of endocolonies in the spleen. The gel filtration method was used to obtain an active fraction which was subjected to chromatography on cation exchanger to give two more fractions. Active was the fraction with basic components.     

Synthesis of oligoribonucleotides by the N-phosphonate method using alkali-labile 2'-o-protective groups. II. Various aspects of using 2'-o-benzoyl and anisole protective groups

The N-acyl, 5'-O-trityl (MeOTr, (MeO)2Tr, Me3Tr), 2'-O-benzoyl (and anisole) nucleosides were prepared by selective aroylation of N,5'-protected nucleosides. By means of the reverse-phase microcolumn liquid chromatography it was shown that the rate of the aryl 2'----3'-isomerisation is lower in case of 2'-anisoylnucleosides and depends on structure of the 5'-O-protecting group. The prepared synthons were used for the manual H-phosphonate solid-phase synthesis of oligoribonucleotides (6-10-mers).     

Anaphylactogenic, antigeni and avid properties of the antibotulin serum from the blood of various animal species]

It was shown that purified and concentrated by the "Diaferm" method antibotulin sera from horse and cattle blood failed to differ by anaphylactogenic properties; at the same time in sensitization of the organism to protein of one animal species the use of the sera of another species provided a lesser reactogenicity of the preparation. The antigenic activity of the purified and concentrated sera from the blood or horses and cows in testing on rabbits was identical, but in response to cow alpha-globulin the animals responsed by a more intensive production of precipitins. The activity of cow and horse antibotulin serum (determined by the rate and stability of their association with the corresponding toxin) proved to be identical.     

Book reviews

Ohne Zusammenfassung

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Book reviews

     

Genetic studies of phosphoglucomutase-1 (PGM1) subtypes: population aspects]

Distribution of the subtypes and gene frequencies of phosphoglucomutase-1 among some populations of Buryats, Kirghizes of the Pamir and Russians of Moscow district was analysed. The frequencies of PGM1 genes vary in Buryats being PGM1+(1) 0.647-0.743, PGM1-(1)-0.100-0.132, PGM2+(1)-0.122-0.199 and PGM2-(1)-0.007-0.037. Following frequencies of PGM1 genes were established for Kirghizes: PGM1+(1) = 0.614, PGM1-(1) = 0.114, PGM2+(1) = 0.217 and PGM2-(1) = 0.054; in Russian populations the frequencies were: PGM1+(1) = 0.578, PGM1-(1) = 0.110, PGM2+(1) = 0.253 and PGM2-(1) = 0.059. Peculiarities of PGM1 polymorphism in the USSR and all over the world were analysed. Parallel biodemographic investigations in Buryat population demonstrated differences in intensities of selection, related to concrete PGM genotypes.     

Ultrastructure of the hypophysiotropic region of the hypothalamus in early ontogenesis in rats]

The results of electron microscopic studies have shown that the 16--18 days old rat embryos already have in the hypophysiotropic area some structures necessary for the realization of neuroendocrine regulations. In the arcuate nuclei, the neurosecretory cells differentiate which are capable to synthesize specific neurosecretory granules of 800--1,000 A in diameter. In the median eminence, the primary portal capillaries develop with which tanicytes and a few axon terminals make contact. One can see in the tanicytes the signs of active transport and accumulation of electron dense polymorphic material. All these phenomena are strengthen during the subsequent development. Hence, several days are before birth the neurosecretory and glial elements of the embryos show the signs of functional activity which strengthen during ontogenesis and are expressed most distinctly in the adult animals.     

A bioelectrochemical study of a suspension of Escherichia coli cells metabolizing glucose and lactose

The metabolism of glucose and lactose in Escherichia coli K-12 cells has been studied using a bioelectrochemical (BEC) approach. The magnitude and the duration of the response of a BEC anode were found to be functions of the composition of nutrient media and the concentration of bacterial cells. The amount of electricity that is generated enzymatically during the metabolism of a particular substrate depends on the activity of the relevant enzymes. This suggests that the BEC approach can be used for evaluating the activity of particular enzyme systems.