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991.
Assessing the scale-specific importance of niches and other spatial processes on beta diversity: a case study from a temperate forest 总被引:1,自引:0,他引:1
Niche processes and other spatial processes, such as dispersal, may simultaneously control beta diversity, yet their relative
importance may shift across spatial and temporal scales. Although disentangling the relative importance of these processes
has been a continuing methodological challenge, recent developments in multi-scale spatial and temporal modeling can now help
ecologists estimate their scale-specific contributions. Here we present a statistical approach to (1) detect the presence
of a space–time interaction on community composition and (2) estimate the scale-specific importance of environmental and spatial
factors on beta diversity. To illustrate the applicability of this approach, we use a case study from a temperate forest understory
where tree seedling abundances were monitored during a 9-year period at 40 permanent plots. We found no significant space–time
interaction on tree seedling composition, which means that the spatial abundance patterns did not vary over the study period.
However, for a given year the relative importance of niche processes and other spatial processes was found to be scale-specific.
Tree seedling abundances were primarily controlled by a broad-scale environmental gradient, but within the confines of this
gradient the finer scale patchiness was largely due to other spatial processes. This case study illustrates that these two
sets of processes are not mutually exclusive and can affect abundance patterns in a scale-dependent manner. More importantly,
the use of our methodology for future empirical studies should help in the merging of niche and neutral perspectives on beta
diversity, an obvious next step for community ecology.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
992.
The search for C allotypes as defined by the markers common to domestic rabbits (Oryctolagus) was extended to additional lagomorphs of the familiesLeporidae andOchotonidae. None of theOchotona sera obtained from Afghanistan and Iran (Ochotona rufescens) exhibited the d11, d12, or e14 allotypes. The e15 marker was detected only by radioimmune binding assays. Comparative inhibition of binding assays revealed similarities with other lagomorphs with respect to the e15i determinant. As in some variants of hare e15 markers, the e15j determinant is absent inOchotona. The results provided a revised phylogenetic scheme for the evolution of the C gene on the basis of the e-series allotypes. 相似文献
993.
Lartigue A Campanacci V Roussel A Larsson AM Jones TA Tegoni M Cambillau C 《The Journal of biological chemistry》2002,277(35):32094-32098
Chemosensory proteins (CSPs) are believed to be involved in chemical communication and perception. Such proteins, of M(r) 13,000, have been isolated from several sensory organs of a wide range of insect species. Several CSPs have been identified in the antennae and proboscis of the moth Mamestra brassicae. One of them, CSPMbraA6, a 112-amino acid antennal protein, has been expressed in large quantities and is soluble in the Escherichia coli periplasm. X-ray structure determination has been performed in parallel with ligand binding assays using tryptophan fluorescence quenching. The protein has overall dimensions of 25 x 30 x 32 A and exhibits a novel type of alpha-helical fold with six helices connected by alpha-alpha loops. A narrow channel extends within the protein hydrophobic core. Fluorescence quenching with brominated alkyl alcohols or fatty acids and modeling studies indicates that CSPMbraA6 is able to bind such compounds with C12-18 alkyl chains. These ubiquitous proteins might have the role of extracting hydrophobic linear compounds (pheromones, odors, or fatty acids) dispersed in the phospholipid membrane and transporting them to their receptor. 相似文献
994.
Activation of MAPK homologues by elicitors in tobacco cells 总被引:17,自引:3,他引:17
Angela Lebrun-Garcia Fatma Ouaked Annick Chiltz Alain Pugin 《The Plant journal : for cell and molecular biology》1998,15(6):773-781
Elicitors of plant defence reactions (such as cryptogein, an elicitin produced by Phytophthora cryptogea , or oligogalacturonides (OGs)), induced in tobacco cell suspensions ( Nicotiana tabacum var Xanthi) a rapid and transient activation of two protein kinases (PKs) with apparent molecular masses of 50 and 46 kDa, respectively. These PKs activated and phosphorylated at tyrosine residues, phosphorylated myelin basic protein (MBP) at serine/threonine residues. Both are recognized by anti-MAPK antibodies. The two MBP kinases possessed the same kinetics of activation, and their activation depended, to the same extent, on different exogenously applied compounds (staurosporine, lanthanum, EGTA). We demonstrate here that the activation of the MBP kinases is calcium dependent and sensitive to staurosporine, a protein kinase inhibitor which annihilates all known responses of tobacco cells to cryptogein. The activation of MBP kinases appeared to be independent of the production of active oxygen species (AOS) and insensitive to calyculin A, a protein phosphatase type 1 and 2A inhibitor. The activation of MAPKs is discussed in relation to the early responses induced by cryptogein. 相似文献
995.
Objective
Aging is associated with increased oxidative stress levels and impaired neovascularization following ischemia. CuZnSOD has an important role to limit oxidative stress in the vasculature. Here we investigated the role of CuZnSOD for the modulation of ischemia-induced neovascularisation during aging.Methods and Results
Hindlimb ischemia was surgically induced in young (2- month-old) or older (8-month-old) wild type (WT) and CuZnSOD−/− mice. We found that blood flow recovery after ischemia and vascular density in ischemic muscles were significantly reduced in older compared to young WT mice. Both in young and older mice, CuZnSOD deficiency led to a further reduction of neovascularization. Accordingly, the resulting neovascularisation potential in a young CuZnSOD−/− mouse was similar to that of an older WT mouse. Oxidative stress levels were also increased to similar levels in the ischemic muscles of young CuZnSOD−/− and older WT mice. To identify potential mechanisms involved, we investigated the effect of aging and CuZnSOD deficiency on the number and the function of endothelial progenitor cells (EPCs). Both aging and CuZnSOD deficiency were associated with reduced number of bone marrow and peripheral EPCs. The effect of moderate aging alone on specific functional activities of EPCs (migration, integration into tubules) was modest. However, CuZnSOD deficiency was associated with severe age-dependent defects in EPC functional activities.Conclusions
CuZnSOD deficiency is associated with accelerated vascular aging and impaired ischemia-induced neovascularization. Our results suggest that in the context of aging, CuZnSOD has an essential role to protect against excessive oxidative stress in ischemic tissues and preserve the function of EPCs. 相似文献996.
997.
Severino Michelin Isabelle Varlet Ccile Martinerie Bernard Perbal Alain Sarasin Horacio G. Surez 《Experimental cell research》1991,196(2)
Human Xeroderma pigmentosum “normal” fibroblasts AS16 (XP4 VI) were transformed after transfection with a recombinant v-myb clone. In this clone (pKXA 3457) derived from avian myeloblastosis virus (AMV), the expression of the oncogene sequences is driven by the AMV U-5 LTR promoter. The transformed cells (ASKXA), which have integrated a rearranged v-myb oncogene, grow in agar, are not tumorigenic in nude mice, and express a 45-kDa v-myb protein. The HMW DNA of these cells transform chicken embryo fibroblasts. The c-Ha-ras oncogene is overexpressed in the ASKXA cells but not in the parental “normal” AS16 cells and a revertant clone (ASKXA Cl 1.1 G). Our results lead to the conclusion that the XP fibroblasts are phenotipically transformed by the presence of the transfected v-myb oncogene, which is able to induce an overexpression of the c-Ha-ras gene. 相似文献
998.
Reyes Babiano Gwenael Badis Cosmin Saveanu Abdelkader Namane Antonia Doyen Antonio Díaz-Quintana Alain Jacquier Micheline Fromont-Racine Jesús de la Cruz 《Nucleic acids research》2013,41(20):9461-9470
Ribosome biogenesis requires >300 assembly factors in Saccharomyces cerevisiae. Ribosome assembly factors Imp3, Mrt4, Rlp7 and Rlp24 have sequence similarity to ribosomal proteins S9, P0, L7 and L24, suggesting that these pre-ribosomal factors could be placeholders that prevent premature assembly of the corresponding ribosomal proteins to nascent ribosomes. However, we found L7 to be a highly specific component of Rlp7-associated complexes, revealing that the two proteins can bind simultaneously to pre-ribosomal particles. Cross-linking and cDNA analysis experiments showed that Rlp7 binds to the ITS2 region of 27S pre-rRNAs, at two sites, in helix III and in a region adjacent to the pre-rRNA processing sites C1 and E. However, L7 binds to mature 25S and 5S rRNAs and cross-linked predominantly to helix ES7Lb within 25S rRNA. Thus, despite their predicted structural similarity, our data show that Rlp7 and L7 clearly bind at different positions on the same pre-60S particles. Our results also suggest that Rlp7 facilitates the formation of the hairpin structure of ITS2 during 60S ribosomal subunit maturation. 相似文献
999.
Fran?ois Lefebvre Géraldine Fazio Béatrice Mounaix Alain J. Crivelli 《Proceedings. Biological sciences / The Royal Society》2013,280(1754)
Quantifying the fitness cost that parasites impose on wild hosts is a challenging task, because the epidemiological history of field-sampled hosts is often unknown. In this study, we used an internal marker of the parasite pressure on individual hosts to evaluate the costs of parasitism with respect to host body condition, size increase and reproductive potential of field-collected animals for which we also determined individual age. In our investigated system, the European eel Anguilla anguilla and the parasitic invader Anguillicoloides crassus, high virulence and severe impacts are expected because the host lacks an adaptive immune response. We demonstrated a nonlinear relationship between the severity of damage to the affected organ (i.e. the swimbladder, our internal marker) and parasite abundance and biomass, thus showing that the use of classical epidemiological parameters was not relevant here. Surprisingly, we found that the most severely affected eels (with damaged swimbladder) had greater body length and mass (+11% and +41%, respectively), than unaffected eels of same age. We discuss mechanisms that could explain this finding and other counterintuitive results in this host–parasite system, and highlight the likely importance of host panmixia in generating great inter-individual variability in growth potential and infection risk. Under that scenario, the most active foragers would not only have the greatest size increase, but also the highest probability of becoming repeatedly infected—via trophic parasite transmission—during their continental life. 相似文献
1000.