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111.
112.
The human glutathione S-transferases: Developmental aspects of the GST1, GST2, and GST3 loci 总被引:6,自引:0,他引:6
Richard C. Strange Brian A. Davis Charles G. Faulder William Cotton A. D. Bain D. A. Hopkinson Robert Hume 《Biochemical genetics》1985,23(11-12):1011-1028
The expression of the GST1, GST2, and GST3 loci in fetal, neonatal, and infant tissues has been studied using starch gel electrophoresis and chromatofocusing. Each locus demonstrated developmental changes in expression, some of which were specific to a single tissue while others occurred in several tissues. GST1 was not usually expressed in any of the tissues studied before 30 weeks of gestation but steadily increased thereafter until adult levels were reached in late infancy. In neonates and older infants the frequencies of the GST1*0, GST1*1, and GST1*2 alleles were 0.79, 0.07, and 0.14, respectively. GST2 was always expressed in liver and adrenal but was only weakly expressed in spleen, cardiac muscle, and diaphragm. In kidney this locus was not usually expressed until nearly 1 year after birth. The GST3 isoenzymes were present in all fetal, neonatal, and infant tissues, although their expression in liver decreased after 30 weeks of gestation. Other isoenzymes with fast anodal mobilities were also identified in several tissues; these are believed to be GST3 isoenzymes that have undergone posttranslational modification rather than products of the putative GST4 locus. No specifically fetal isoenzymes were detected. 相似文献
113.
Thomas W. Johnson Andrew D. Mumford Lauren J. Scott Stuart Mundell Mark Butler Julian W. Strange Chris A. Rogers Barnaby C. Reeves Andreas Baumbach 《PloS one》2015,10(12)
Background
Rapid coronary recanalization following ST-elevation myocardial infarction (STEMI) requires effective anti-platelet and anti-thrombotic therapies. This study tested the impact of door to end of procedure (‘door-to-end’) time and baseline platelet activity on platelet inhibition within 24hours post-STEMI.Methods and Findings
108 patients, treated with prasugrel and procedural bivalirudin, underwent Multiplate® platelet function testing at baseline, 0, 1, 2 and 24hours post-procedure. Major adverse cardiac events (MACE), bleeding and stent thrombosis (ST) were recorded. Baseline ADP activity was high (88.3U [71.8–109.0]), procedural time and consequently bivalirudin infusion duration were short (median door-to-end time 55minutes [40–70] and infusion duration 30minutes [20–42]). Baseline ADP was observed to influence all subsequent measurements of ADP activity, whereas door-to-end time only influenced ADP immediately post-procedure. High residual platelet reactivity (HRPR ADP>46.8U) was observed in 75% of patients immediately post-procedure and persisted in 24% of patients at 2hours. Five patients suffered in-hospital MACE (4.6%). Acute ST occurred in 4 patients, all were <120mins post-procedure and had HRPR. No significant bleeding was observed. In a post-hoc analysis, pre-procedural morphine use was associated with significantly higher ADP activity following intervention.Conclusions
Baseline platelet function, time to STEMI treatment and opiate use all significantly influence immediate post-procedural platelet activity. 相似文献114.
Bombus bifarius is a widespread bumble bee that occurs in montane regions of western North America. This species has several major color pattern polymorphisms and shows evidence of genetic structuring among regional populations, and the taxonomic status of regional populations has repeatedly been debated. We test whether observed structure is evidence for discrete gene flow barriers that might indicate isolation or instead reflects clinal variation associated with spatially limited dispersal in a complex landscape. We first consider color pattern variation and identify geographical patterns of B. bifarius color variation using cluster analysis. We then use climate data and a comprehensive set of B. bifarius natural history records with an existing genetic data set to model the distribution of environmentally suitable habitat in western North America and predict pathways of potential gene flow using circuit theory. Resistance distances among populations that incorporate environmental suitability information predict patterns of genetic structure much better than geographic distance or Bayesian clustering alone. Results suggest that there may not be barriers to gene flow warranting further taxonomic considerations, but rather that the arrangement of suitable habitat at broad scales limits dispersal sufficiently to explain observed levels of population differentiation in B. bifarius. 相似文献
115.
W. Edwin Harris Selvino R. de Kort Caroline M. Bettridge Joana Borges Bradley Cain Hamadi I. Dulle Robert Fyumagwa Donatua Gadiye Martin Jones Ladislaus Kahana Julius Kibebe Jafari R. Kideghesho Fidelcastor F. Kimario Alex Kisingo Francis Makari Emmanuel Martin Andimile Martin Masuruli B. Masuruli Kokel Melubo Hannah L. Mossman Linus Munishi Reginald Mwaya Robert Nasi Oliver Nyakunga Elizabeth Price Rehema A. Shoo Emily F. Strange Elias Symeonakis John E. Fa 《African Journal of Ecology》2021,59(1):326-331
116.
Huang CG Lamitina T Agre P Strange K 《American journal of physiology. Cell physiology》2007,292(5):C1867-C1873
117.
Mateusz Siedlinski Barbara Klanderman Robert A. Sandhaus Alan F. Barker Mark L. Brantly Edward Eden N. Gerard McElvaney Stephen I. Rennard James M. Stocks James K. Stoller Charlie Strange Gerard M. Turino Edward J. Campbell Dawn L. DeMeo 《Epigenetics》2012,7(7):720-728
Alpha-1 antitrypsin (AAT) deficiency and tobacco smoking are confirmed risk factors for Chronic Obstructive Pulmonary Disease. We hypothesized that variable DNA methylation would be associated with smoking and inflammation, as reflected by the level of C-Reactive Protein (CRP) in AAT-deficient subjects. Methylation levels of 1,411 autosomal CpG sites from the Illumina GoldenGate Methylation Cancer Panel I were analyzed in 316 subjects. Associations of five smoking behaviors and CRP levels with individual CpG sites and average methylation levels were assessed using non-parametric testing, linear regression and linear mixed effect models, with and without adjustment for age and gender. Univariate linear regression analysis revealed that methylation levels of 16 CpG sites significantly associated with ever-smoking status. A CpG site in the TGFBI gene was the only site associated with ever-smoking after adjustment for age and gender. No highly significant associations existed between age at smoking initiation, pack-years smoked, duration of smoking, and time since quitting smoking as predictors of individual CpG site methylation levels. However, ever-smoking and younger age at smoking initiation associated with lower methylation level averaged across all sites. DNA methylation at CpG sites in the RUNX3, JAK3 and KRT1 genes associated with CRP levels. The most significantly associated CpG sites with gender and age mapped to the CASP6 and FZD9 genes, respectively. In summary, this study identified multiple potential candidate CpG sites associated with ever-smoking and CRP level in AAT-deficient subjects. Phenotypic variability in Mendelian diseases may be due to epigenetic factors. 相似文献
118.
119.
Hiroaki Miyazaki Toshiki Yamada Angela Parton Rebecca Morrison Sunghoon Kim Albert?H. Beth Kevin Strange 《Biophysical journal》2012,103(8):1706-1718
The signaling mechanisms that regulate CLC anion channels are poorly understood. Caenorhabditis elegans CLH-3b is a member of the CLC-1/2/Ka/Kb channel subfamily. CLH-3b is activated by meiotic cell-cycle progression and cell swelling. Inhibition is brought about by GCK-3 kinase-mediated phosphorylation of S742 and S747 located on a ∼176 amino acid disordered domain linking CBS1 and CBS2. Much of the inter-CBS linker is dispensable for channel regulation. However, deletion of a 14 amino acid activation domain encompassing S742 and S747 inhibits channel activity to the same extent as GCK-3. The crystal structure of CmCLC demonstrated that CBS2 interfaces extensively with an intracellular loop connecting membrane helices H and I, the C-terminus of helix D, and a short linker connecting helix R to CBS1. Point mutagenesis of this interface identified two highly conserved aromatic amino acid residues located in the H-I loop and the first α-helix (α1) of CBS2. Mutation of either residue to alanine rendered CLH-3b insensitive to GCK-3 inhibition. We suggest that the dephosphorylated activation domain normally interacts with CBS1 and/or CBS2, and that conformational information associated with this interaction is transduced through a conserved signal transduction module comprising the H-I loop and CBS2 α1. 相似文献
120.
Maud Poisbleau Laurent Demongin Ian J. Strange Helen Otley Petra Quillfeldt 《Polar Biology》2008,31(8):925-932
The rockhopper penguins Eudyptes chrysocome have recently been split into the northern E. moseleyi and the southern E. chrysocome rockhopper penguin. It is therefore crucial to have a comprehensive understanding of the biology of each species in order
to develop appropriate conservation measures. We investigated the breeding biology of the southern rockhopper on New Island,
in the western part of the Falklands Islands, by following the breeding attempt of 160 pairs during the 2006/2007 season and
examining the effect of lay time and colony habitat on breeding success. Specifically, we compared survival and growth parameters
between A- and B-eggs and chicks from non-manipulated and artificially manipulated nests to investigate why southern rockhopper
penguins in the Falkland Islands are more able to fledge an A-egg (first laid) than conspecifics elsewhere. Breeding was highly
synchronous, with no significant difference in the breeding success between early and late breeders or between pairs breeding
in different habitats. We demonstrate for the first time that the A-egg produced by the southern rockhopper penguin has, when
alone, the same theoretical intrinsic potential to lead to a fledged chick as the B-egg. In contrast, the hatching success
and survival of the B-chick was similar when alone or in a two-egg clutch. 相似文献