Hierarchical regulation of pelagic invertebrates in lakes of the northern Great Plains: a novel model for interdecadal effects of future climate change on lakes

Endorheic lakes of the northern Great Plains encompass a wide range of environmental parameters (e.g., salinity, pH, DOC, Ca, nutrients, depth) that vary 1000‐fold among sites and through the past 2000 years due to variation in basin hydrology and evaporative forcing. However, while many environmental parameters are known to individually influence zooplankton diversity and taxonomic composition, relatively little is known of the hierarchical relationships among potential controls or of how regulatory mechanisms may change in response to climate variation on diverse scales. To address these issues, we surveyed 70 lakes within a 100 000 km² prairie region to simulate the magnitude of environmental change expected to occur over 100–1000 years and to quantify the unique and interactive effects of diverse environmental parameters in regulating pelagic invertebrate community structure at that scale. Multivariate analyses showed that salinity was the principal correlate of changes in invertebrate composition among lakes, with a sequential loss of taxa between salinities of 4 and 50 g total dissolved solids L^?1 until one to two species predominated in highly saline systems. In contrast, changes in the concentrations of Ca²⁺ and other mineral nutrients exerted secondary controls of invertebrate assemblages independent of salinity, whereas lake depth provided a tertiary regulatory mechanism structuring species composition. In contrast to these large‐scale hierarchical patterns, seasonal surveys (May, July, September) of a subset of 21 lakes in each of 2003–2005 revealed that annual meteorological variation had no measurable effect on pelagic invertebrates, despite large differences in temperature, precipitation, and evaporation arising from regional droughts. Together these findings show that pelagic invertebrate communities in saline lakes are resilient to interannual variability in climate, but suggest that lakes of the northern Great Plains may provide a sensitive model to forecast centennial effects of future climate change.     

Local selection modifies phenotypic divergence among Rana temporaria populations in the presence of gene flow

In ectotherms, variation in life history traits among populations is common and suggests local adaptation. However, geographic variation itself is not a proof for local adaptation, as genetic drift and gene flow may also shape patterns of quantitative variation. We studied local and regional variation in means and phenotypic plasticity of larval life history traits in the common frog Rana temporaria using six populations from central Sweden, breeding in either open‐canopy or partially closed‐canopy ponds. To separate local adaptation from genetic drift, we compared differentiation in quantitative genetic traits (Q_ST) obtained from a common garden experiment with differentiation in presumably neutral microsatellite markers (F_ST). We found that R. temporaria populations differ in means and plasticities of life history traits in different temperatures at local, and in F_ST at regional scale. Comparisons of differentiation in quantitative traits and in molecular markers suggested that natural selection was responsible for the divergence in growth and development rates as well as in temperature‐induced plasticity, indicating local adaptation. However, at low temperature, the role of genetic drift could not be separated from selection. Phenotypes were correlated with forest canopy closure, but not with geographical or genetic distance. These results indicate that local adaptation can evolve in the presence of ongoing gene flow among the populations, and that natural selection is strong in this system.     

Distribution of acid and vanadium in Rhopalaea birkelandi Tokioka

The distribution of acidity, vanadium, and fouling in Rhopalaea birkelandi Tokioka, a solitary ascidian common on the Pacific coast of Central America, was investigated. The tunic around the siphons released acid (pH ?2) when slightly bruised and was not fouled. However, the basal parts of the tunic, which were non-acidic, were fouled. Whole R. birkelandi has ≈610 ppm vanadium dry wt; the zooid with the tunic removed has ≈ 1956 ppm and the tunic has ≈ 500 ppm vanadium dry wt. Vanadocytes were present. These data are discussed in reference to previous work on the roles of vanadium and acid in benthic ascidians.     

Effect of small-scale shear on grazing and growth of the dinoflagellate Pfiesteria piscicida

Low turbulence environments have been hypothesized to be necessary for toxic outbreaks of the heterotrophic/mixotrophic dinoflagellate Pfiesteria piscicida. A toxic Pfiesteria outbreak occurred in the shallow waters (the flats) of the lower Pocomoke River, MD, USA, in 1997. During August 1999 and May and August 2000, data were collected with a Shallow Water ADV Turbulence Tripod (SWATT) to estimate turbulent shear at a location monitored for Pfiesteria on the flats. Approximately 78% of the observed shears were ≤1 s⁻¹ and 98% were ≤2 s⁻¹. Densities of Pfiesteria-like dinoflagellates were low in the Pocomoke River during 1999 and 2000 and no toxic outbreaks occurred. In the laboratory, Couette cylinders were used to determine the effect of small-scale shear on feeding and growth of cultured P. piscicida growing on cryptophyte prey. Shear of 1 s⁻¹ had little or no effect on feeding or growth but 3 s⁻¹ reduced feeding and survival/growth of Pfiesteria zoospores. Small, rapidly dividing Pfiesteria cells were most susceptible to negative effects of shear. Pfiesteria appears to be more sensitive to small-scale shear than are most autotrophic dinoflagellates. However, in the lower Pocomoke River, turbulent shear is rarely high enough to inhibit the growth of non-toxic Pfiesteria zoospores. Toxic functional types (TOX-A and TOX-B) may be more sensitive to small-scale shear; it will be important to determine the responses of these types of Pfiesteria in order to predict the affects of wind and tidal mixing on toxic outbreaks.     

Old Crop,New Society: Persistence and Change of Tartary Buckwheat Farming in Yunnan,China

Traditional agricultural systems are important for crop genetic resources conservation. Many scholars have addressed the problem of traditional cultivar replacement by modern varieties, but few have investigated the entire loss of traditional crops from farming systems. Our prior research suggested that tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) agriculture is rapidly decreasing in Yunnan China, the center of the crop’s origin and an important repository of on-farm genetic diversity. Using interdisciplinary methodology to determine whether the crop is indeed in decline and evaluate reasons behind planting trends, we found a combination of interacting agroecological, socio-cultural, and institutional factors influence tartary buckwheat planting in Yunnan. Farmer ethnicity, presence of a commercial market for the crop, and government agricultural policy were particularly important. Low commercial value of the crop compared to other alternatives, reduction of available farmland, and labor shortages were major reasons cited for crop abandonment. Despite an overall reduction in tartary buckwheat planting across a wide spectrum of farms, we also found the crop retains subsistence, rotational and cultural value for many Yunnan farmers. We conclude that although Yunnan farms are increasingly managed commercially rather than for subsistence, tartary buckwheat will continue to occupy a niche in the agricultural landscape.     

Screening Global Positioning System Location Data for Errors Using Animal Movement Characteristics

Abstract: Animal locations estimated by Global Positioning System (GPS) inherently contain errors. Screening procedures used to remove large positional errors often trade data accuracy for data loss. We developed a simple screening method that identifies locations arising from unrealistic movement patterns. When applied to a large data set of moose (Alces alces) locations, our method identified virtually all known errors with minimal loss of data. Thus, our method for screening GPS data improves the quality of data sets and increases the value of such data for research and management.     

Bill morphology reflects adaptation to a fibrous diet in the kākāpō (Strigops: Psittaciformes)

We describe the upper portion of the bill sheath (rhinotheca) of the kākāpō (Strigops habroptilus) from three adult female specimens. The external buccal surface of the rhinotheca is deeply concave with a prominent palatal stop and hardened chevrons creating a ‘milling apparatus’ that the kākāpō uses to grind food. The palatal stop presents a working face of 40–50?mm². The internal surface of the rhinotheca mirrors the overlying premaxilla and provides a distinct thickened abutment consistent with resistance against the increased workload of the mandibles (gnathotheca) due to the kākāpō’s fibrous diet and chewing style. Along the midline, the rhinotheca at the abutment is up to 5.6?mm thick, compared with as thin as 2.1?mm elsewhere on the midline. The closely related Nestor parrots have less developed palatal stops, chevrons and abutments on their rhinothecas consistent with their lower preference for fibrous plant material. The form of the rhinotheca agrees with the kākāpō’s feeding ecology as a generalist herbivore that grinds locally available fibrous material to assist digestion.     

A straightforward DOPE (double labeling of oligonucleotide probes)-FISH (fluorescence in situ hybridization) method for simultaneous multicolor detection of six microbial populations

Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is an essential tool for the cultivation-independent identification of microbes within environmental and clinical samples. However, one of the major constraints of conventional FISH is the very limited number of different target organisms that can be detected simultaneously with standard epifluorescence or confocal laser scanning microscopy. Recently, this limitation has been overcome via an elegant approach termed combinatorial labeling and spectral imaging FISH (CLASI-FISH) (23). This technique, however, suffers compared to conventional FISH from an inherent loss in sensitivity and potential probe binding biases caused by the competition of two differentially labeled oligonucleotide probes for the same target site. Here we demonstrate that the application of multicolored, double-labeled oligonucleotide probes enables the simultaneous detection of up to six microbial target populations in a straightforward and robust manner with higher sensitivity and less bias. Thus, this newly developed technique should be an attractive option for all researchers interested in applying conventional FISH methods for the study of microbial communities.     

Ocean acidification with (de)eutrophication will alter future phytoplankton growth and succession

Human activity causes ocean acidification (OA) though the dissolution of anthropogenically generated CO₂ into seawater, and eutrophication through the addition of inorganic nutrients. Eutrophication increases the phytoplankton biomass that can be supported during a bloom, and the resultant uptake of dissolved inorganic carbon during photosynthesis increases water-column pH (bloom-induced basification). This increased pH can adversely affect plankton growth. With OA, basification commences at a lower pH. Using experimental analyses of the growth of three contrasting phytoplankton under different pH scenarios, coupled with mathematical models describing growth and death as functions of pH and nutrient status, we show how different conditions of pH modify the scope for competitive interactions between phytoplankton species. We then use the models previously configured against experimental data to explore how the commencement of bloom-induced basification at lower pH with OA, and operating against a background of changing patterns in nutrient loads, may modify phytoplankton growth and competition. We conclude that OA and changed nutrient supply into shelf seas with eutrophication or de-eutrophication (the latter owing to pollution control) has clear scope to alter phytoplankton succession, thus affecting future trophic dynamics and impacting both biogeochemical cycling and fisheries.