全文获取类型
收费全文 | 6507篇 |
免费 | 776篇 |
国内免费 | 4篇 |
出版年
2021年 | 81篇 |
2019年 | 52篇 |
2018年 | 75篇 |
2017年 | 58篇 |
2016年 | 107篇 |
2015年 | 186篇 |
2014年 | 180篇 |
2013年 | 267篇 |
2012年 | 280篇 |
2011年 | 344篇 |
2010年 | 200篇 |
2009年 | 174篇 |
2008年 | 267篇 |
2007年 | 252篇 |
2006年 | 217篇 |
2005年 | 253篇 |
2004年 | 223篇 |
2003年 | 226篇 |
2002年 | 212篇 |
2001年 | 178篇 |
2000年 | 148篇 |
1999年 | 160篇 |
1998年 | 101篇 |
1997年 | 75篇 |
1996年 | 79篇 |
1995年 | 68篇 |
1994年 | 83篇 |
1993年 | 69篇 |
1992年 | 125篇 |
1991年 | 116篇 |
1990年 | 136篇 |
1989年 | 118篇 |
1988年 | 118篇 |
1987年 | 98篇 |
1986年 | 86篇 |
1985年 | 97篇 |
1984年 | 90篇 |
1983年 | 72篇 |
1982年 | 76篇 |
1981年 | 84篇 |
1980年 | 64篇 |
1979年 | 83篇 |
1978年 | 60篇 |
1977年 | 54篇 |
1976年 | 58篇 |
1975年 | 65篇 |
1974年 | 64篇 |
1973年 | 70篇 |
1972年 | 64篇 |
1968年 | 51篇 |
排序方式: 共有7287条查询结果,搜索用时 0 毫秒
991.
Maria Loreto Contador Louise H. Comas Samuel G. Metcalf William L. Stewart Ignacio Porris Gomez Claudia Negron Bruce D. Lampinen 《Annals of botany》2015,116(1):49-60
Background and Aims Examination of plant growth below ground is relatively scant compared with that above ground, and is needed to understand whole-plant responses to the environment. This study examines whether the seasonal timing of fine root growth and the spatial distribution of this growth through the soil profile varies in response to canopy manipulation and soil temperature.Methods Plasticity in the seasonal timing and vertical distribution of root production in response to canopy and soil water manipulation was analysed in field-grown walnut (Juglans regia ‘Chandler’) using minirhizotron techniques.Key Results Root production in walnuts followed a unimodal curve, with one marked flush of root growth starting in mid-May, with a peak in mid-June. Root production declined later in the season, corresponding to increased soil temperature, as well as to the period of major carbohydrate allocation to reproduction. Canopy and soil moisture manipulation did not influence the timing of root production, but did influence the vertical distribution of roots through the soil profile. Water deficit appeared to promote root production in deeper soil layers for mining soil water. Canopy removal appeared to promote shallow root production.Conclusions The findings of this study add to growing evidence that root growth in many ecosystems follows a unimodal curve with one marked flush of root growth in coordination with the initial leaf flush of the season. Root vertical distribution appeared to have greater plasticity than timing of root production in this system, with temperature and/or carbohydrate competition constraining the timing of root growth. Effects on root distribution can have serious impacts on trees, with shallow rooting having negative impacts in years with limited soil water or positive impacts in years with wet springs, and deep rooting having positive impacts on soil water mining from deeper soil layers but negative impacts in years with wet springs. 相似文献
992.
Valley Stewart 《Molecular microbiology》2014,91(5):853-857
Proteolytic control of Caulobacter cell cycle proteins is primarily executed by ClpXP, a dynamically localized protease implicated in turnover of several factors critical for faithful cell cycle progression. Here, we show that the transient midcell localization of ClpXP that precedes cytokinesis requires the FtsZ component of the divisome. Although ClpAP does not exhibit subcellular localization, FtsZ is a substrate of both ClpXP and ClpAP in vivo and in vitro. A peptide containing the C‐terminal portion of the FtsA divisome protein is a substrate of both ClpXP and ClpAP in vitro but is primarily degraded by ClpAP in vivo. Caulobacter carries out an asymmetric division in which FtsZ and FtsA are stable in stalked cells but degraded in the non‐replicative swarmer cell where ClpAP alone degrades FtsA and both ClpAP and ClpXP degrade FtsZ. While asymmetric division in Caulobacter normally yields larger stalked and smaller swarmer daughters, we observe a loss of asymmetric size distribution among daughter cells when clpA is depleted from a strain in which FtsZ is constitutively produced. Taken together, these results suggest that the activity of both ClpXP and ClpAP on divisome substrates is differentially regulated in daughter cells. 相似文献
993.
994.
995.
996.
Alice Stewart 《BMJ (Clinical research ed.)》1961,1(5224):452-460
997.
J. Stewart MacKintosh 《BMJ (Clinical research ed.)》1928,1(3512):732-733
998.
999.
The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybrid molecules. Restriction fragments of the replicating form of bacteriophage luminal diameter X174 were hybridized to the intact 'plus' strand, thereby forming hybrids having single- and/or double-stranded regions in the kilobase range. A series of such hybrid preparations were subject to caffeine concentration gradient elution from benzoylated DEAE-cellulose. After logarithmic transformation, a linear relationship (R = 0.94) could be demonstrated between eluting caffeine concentration and single-stranded length, irrespective of the length of associated double-stranded regions or the location, within a given fragment, of unpaired nucleotides. Benzoylated DEAE-cellulose chromatography may therefore be used to separate and characterize, on a preparative scale, double-stranded DNA containing single-stranded regions. 相似文献
1000.
The early MIS 3 (55–40 Kyr BP associated with Middle Palaeolithic archaeology) bird remains from Pin Hole, Creswell Crags, Derbyshire, England are analysed in the context of the new dating of the site’s stratigraphy. The analysis is restricted to the material from the early MIS 3 level of the cave because the upper fauna is now known to include Holocene material as well as that from the Late Glacial. The results of the analysis confirm the presence of the taxa, possibly unexpected for a Late Pleistocene glacial deposit including records such as Alpine swift, demoiselle crane and long-legged buzzard with southern and/or eastern distributions today. These taxa are accompanied by more expected ones such as willow ptarmigan /red grouse and rock ptarmigan living today in northern and montane areas. Finally, there are temperate taxa normally requiring trees for nesting such as wood pigeon and grey heron. Therefore, the result of the analysis is that the avifauna of early MIS 3 in England included taxa whose ranges today do not overlap making it a non-analogue community similar to the many steppe-tundra mammalian faunas of the time. The inclusion of more temperate and woodland taxa is discussed in the light that parts of northern Europe may have acted as cryptic northern refugia for some such taxa during the last glacial. These records showing former ranges of taxa are considered in the light of modern phylogeographic studies as these often assume former ranges without considering the fossil record of those taxa. In addition to the anomalous combination of taxa during MIS 3 living in Derbyshire, the individuals of a number of the taxa are different in size and shape to members of the species today probably due to the high carrying capacity of the steppe-tundra. 相似文献