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71.
Sensory information about the outside world is encoded by neurons in sequences of discrete, identical pulses termed action potentials or spikes. There is persistent controversy about the extent to which the precise timing of these spikes is relevant to the function of the brain. We revisit this issue, using the motion-sensitive neurons of the fly visual system as a test case. Our experimental methods allow us to deliver more nearly natural visual stimuli, comparable to those which flies encounter in free, acrobatic flight. New mathematical methods allow us to draw more reliable conclusions about the information content of neural responses even when the set of possible responses is very large. We find that significant amounts of visual information are represented by details of the spike train at millisecond and sub-millisecond precision, even though the sensory input has a correlation time of ~55 ms; different patterns of spike timing represent distinct motion trajectories, and the absolute timing of spikes points to particular features of these trajectories with high precision. Finally, the efficiency of our entropy estimator makes it possible to uncover features of neural coding relevant for natural visual stimuli: first, the system's information transmission rate varies with natural fluctuations in light intensity, resulting from varying cloud cover, such that marginal increases in information rate thus occur even when the individual photoreceptors are counting on the order of one million photons per second. Secondly, we see that the system exploits the relatively slow dynamics of the stimulus to remove coding redundancy and so generate a more efficient neural code.  相似文献   
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X-Ray microanalysis of fully hydrated, bulk-frozen samples was used to measure concentrations of potassium, sodium and chloride in various cell types along seminal roots of barley ( Hordeum vulgare L. cv. California Mariout) seedlings (1 to 150 mm from the tip). In the cytoplasm of all meristematic cells 1 mm from the root tip, the average concentrations of potassium and chloride were ca 200 and 15 m M , respectively. The potassium level was also high in the vacuoles of incipient xylem elements and did not drop to significantly lower values until 10 mm from the tip in protoxylem, 50 mm in early metaxylem and 150 mm in late metaxylem (LMX). Light microscopy observations (Nomarski optics) of hand-cut sections showed the presence of cytoplasmic strands and also the presence of intact cross walls in LMX up to a distance of 100 mm. Both quantitative analysis of ion contents and structural observations suggested that LMX elements act as a large transitional sink of accumulated ions and therefore may not function as a main pathway of transport until perforation of the end wall takes place 100–150 mm from the root tip. Treatment with 50 m M NaCl resulted in higher concentrations of sodium and chloride in LMX elements than in the surrounding cells, suggesting that living xylem elements, which develop a large central vacuole at an early stage of root differentiation, may assist in alleviating salinity stress in the meristematic region of barley root tips. Further, it is proposed that reabsorption of sodium and chloride from the LMX, especially before the disappearance of the cross walls, may provide a means of salinity tolerance.  相似文献   
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Thin slices of beetroot tissue develop long ER lamellae during a period of aerated washing. After 2–3 days crystalloid bodies begin to appear within the ER cisternae and these were shown to consist largely of protein.The effect of inhibitors of RNA and protein synthesis on the formation of ER lamellae and crystals was studied, and results indicate that protein synthesis, but not synthesis of m-RNA, is essential for both processes. It is also suggested that the protein-synthesizing system associated with the ER is more stable than the cytoplasmic polyribosome system, which may require the continued production of m-RNA after slicing.This research was supported by grants from the Australian Research Grants Committee.  相似文献   
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Under appropriate experimental conditions toluidine blue is bound to the yeast cell surface, without penetrating into the cells. Based on experimental observations it is highly probable that the dye is bound to polyphosphates, localized outside the plasma membrane. The probable localization of polyphosphates outside the plasma membrane is important in the context of the proposed involvement of polyphosphates in glucose transport in yeast.  相似文献   
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Chloride concentrations within individual chloroplasts, the adjacent cytoplasm and nearby vacuoles of spinach mesophyll cells (Spinacea oleracea L. cv. Hybrid 102) were determined by means of electron probe X-ray microanalysis in the cleavage plane of quench frozen tissue, which was maintained at liquid nitrogen temperatures. The accuracy of quantitative data obtained with this technique is greatly improved by the adoption of a peak to background ratio method and use of carbon slurry standards, which mimic the quench frozen tissue and its X-ray fluorescence. Chloroplasts were incapable of maintaining relatively high levels of Cl? under conditions of low Cl? availability (zero Cl? or 20 μM Cl? in nutrient solution), and under conditions of Cl? stress (100 or 200 mM Cl?) chloroplasts had only a limited capacity to maintain a Cl? concentration at a level below that of the cytoplasm and vacuole. However, under conditions of Cl? stress the concentration of Cl? in cytoplasm immediately adjacent to chloroplasts was substantially higher than in the chloroplasts or more distant cytoplasm. Thus, Cl? levels in chloroplasts are apparently not as tightly regulated as was suggested by estimates of Cl? concentration based on aqueous isolation of chloroplasts. Levels of S in chloroplasts were relatively high (equivalent to 40–60 mM SO42? in S standards) and constant for all treatments, with the possible exception of lower S levels in chloroplasts of leaves approaching premature senescence as a result of salt stress. It is implied that the stability of the S-content results largely from its presence in macromolecular components of chloroplasts (sulfolipids and proteins).  相似文献   
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2-Deoxy-d-glucose transport and metabolism was studied in Saccharomyces fragilis. Inside the cells four phosphorylated and three non-phosphorylated derivatives were found and identified. Accumulation of phosphorylated 2-deoxyglucose derivatives was balanced by a concomitant decrease of cellular ATP, orthophosphate and polyphosphates.The free sugar was concentrated against a concentration gradient, contradicting facilitated diffusion. Pulse labeling experiments revealed transport-associated phosphorylation.Theoretical considerations and analysis of the effects of iodoacetate showed that an intracellular hexokinase activity was not involved in 2-deoxyglucose phosphorylation, although this sugar is a good substrate for the enzyme in in vitro experiments.  相似文献   
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