全文获取类型
收费全文 | 1503篇 |
免费 | 98篇 |
出版年
2023年 | 6篇 |
2022年 | 21篇 |
2021年 | 39篇 |
2020年 | 21篇 |
2019年 | 23篇 |
2018年 | 31篇 |
2017年 | 34篇 |
2016年 | 38篇 |
2015年 | 73篇 |
2014年 | 73篇 |
2013年 | 102篇 |
2012年 | 110篇 |
2011年 | 102篇 |
2010年 | 72篇 |
2009年 | 65篇 |
2008年 | 87篇 |
2007年 | 90篇 |
2006年 | 75篇 |
2005年 | 63篇 |
2004年 | 52篇 |
2003年 | 62篇 |
2002年 | 68篇 |
2001年 | 22篇 |
2000年 | 31篇 |
1999年 | 21篇 |
1998年 | 16篇 |
1997年 | 7篇 |
1996年 | 4篇 |
1995年 | 9篇 |
1994年 | 10篇 |
1993年 | 7篇 |
1992年 | 9篇 |
1991年 | 6篇 |
1990年 | 10篇 |
1989年 | 9篇 |
1988年 | 19篇 |
1987年 | 8篇 |
1986年 | 10篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1982年 | 4篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1975年 | 5篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1971年 | 4篇 |
1970年 | 5篇 |
排序方式: 共有1601条查询结果,搜索用时 15 毫秒
961.
Stojan Maleschlijski Stella Bauer Alessio Di Fino G. Hernan Sendra Anthony S. Clare Axel Rosenhahn 《Biofouling》2014,30(9):1055-1065
Testing of new coatings to control fouling frequently involves single-species laboratory bioassays. Barnacle cyprids are among the most widely used model organisms in marine biofouling research, and surfaces that inhibit their settlement are considered to be promising candidates for new coating concepts. An analysis of motility parameters (mean velocity and swimming area coefficient) and distribution of cyprids of Balanus amphitrite in different swimming regions in the vicinity of model surfaces (self-assembled monolayers) is presented. The data are correlated with the settlement preferences of cyprids on these surfaces. Cyprids were predominantly found in interfacial regions and the transition frequencies between swimming regions of different depths were determined. 相似文献
962.
The metacommunity concept, describing how local and regional scale processes interact to structure communities, has been successfully applied to patterns of taxonomic diversity. Functional diversity has proved useful for understanding local scale processes, but has less often been applied to understanding regional scale processes. Here, we explore functional diversity patterns within a metacommunity context to help elucidate how local and regional scale processes influence community assembly. We detail how each of the four metacommunity perspectives (species sorting, mass effects, patch dynamics, neutral) predict different patterns of functional beta‐ and alpha‐diversity and spatial structure along two key gradients: dispersal limitation and environmental conditions. We then apply this conceptual model to a case study from alpine tundra plant communities. We sampled species composition in 17 ‘sky islands’ of alpine tundra in the Colorado Rocky Mountains, USA that differed in geographic isolation and area (key factors related to dispersal limitation) and temperature and elevation (key environmental factors). We quantified functional diversity in each site based on specific leaf area, leaf area, stomatal conductance, plant height and chlorophyll content. We found that colder high elevation sites were functionally more similar to each other (decreased functional beta‐diversity) and had lower functional alpha‐diversity. Geographic isolation and area did not influence functional beta‐ or alpha‐diversity. These results suggest a strong role for environmental conditions structuring alpine plant communities, patterns consistent with the species sorting metacommunity perspective. Incorporating functional diversity into metacommunity theory can help elucidate how local and regional factors structure communities and provide a framework for observationally examining the role of metacommunity dynamics in systems where experimental approaches are less tractable. 相似文献
963.
J. S. Stella P. L. Munday S. P. W. Walker M. S. Pratchett G. P. Jones 《Oecologia》2014,174(4):1187-1195
Although mutualisms are ubiquitous in nature, our understanding of the potential impacts of climate change on these important ecological interactions is deficient. Here, we report on a thermal stress-related shift from cooperation to antagonism between members of a mutualistic coral-dwelling community. Increased mortality of coral-defending crustacean symbionts Trapezia cymodoce (coral crab) and Alpheus lottini (snapping shrimp) was observed in response to experimentally elevated temperatures and reduced coral-host (Pocillopora damicornis) condition. However, strong differential numerical effects occurred among crustaceans as a function of species and sex, with shrimp (75 %), and female crabs (55 %), exhibiting the fastest and greatest declines in numbers. These declines were due to forceful eviction from the coral-host by male crabs. Furthermore, surviving female crabs were impacted by a dramatic decline (85 %) in egg production, which could have deleterious consequences for population sustainability. Our results suggest that elevated temperature switches the fundamental nature of this interaction from cooperation to competition, leading to asymmetrical effects on species and/or sexes. Our study illustrates the importance of evaluating not only individual responses to climate change, but also potentially fragile interactions within and among susceptible species. 相似文献
964.
Marilyn Ader Darko Stefanovski Stella P. Kim Joyce M. Richey Viorica Ionut Karyn J. Catalano Katrin Hucking Martin Ellmerer Gregg Van Citters Isabel R. Hsu Jenny D. Chiu Orison O. Woolcott Lisa N. Harrison Dan Zheng Maya Lottati Cathryn M. Kolka Vahe Mooradian Justin Dittmann Sophia Yae Huiwen Liu Ana Valeria B. Castro Morvarid Kabir Richard N. Bergman 《Obesity (Silver Spring, Md.)》2014,22(5):1238-1245
965.
Sabine Kienesberger Hanna Sprenger Stella Wolfgruber Bettina Halwachs Gerhard G. Thallinger Guillermo I. Perez-Perez Martin J. Blaser Ellen L. Zechner Gregor Gorkiewicz 《PloS one》2014,9(1)
Campylobacter fetus are important animal and human pathogens and the two major subspecies differ strikingly in pathogenicity. C. fetus subsp. venerealis is highly niche-adapted, mainly infecting the genital tract of cattle. C. fetus subsp. fetus has a wider host-range, colonizing the genital- and intestinal-tract of animals and humans. We report the complete genomic sequence of C. fetus subsp. venerealis 84-112 and comparisons to the genome of C. fetus subsp. fetus 82-40. Functional analysis of genes predicted to be involved in C. fetus virulence was performed. The two subspecies are highly syntenic with 92% sequence identity but C. fetus subsp. venerealis has a larger genome and an extra-chromosomal element. Aside from apparent gene transfer agents and hypothetical proteins, the unique genes in both subspecies comprise two known functional groups: lipopolysaccharide production, and type IV secretion machineries. Analyses of lipopolysaccharide-biosynthesis genes in C. fetus isolates showed linkage to particular pathotypes, and mutational inactivation demonstrated their roles in regulating virulence and host range. The comparative analysis presented here broadens knowledge of the genomic basis of C. fetus pathogenesis and host specificity. It further highlights the importance of surface-exposed structures to C. fetus pathogenicity and demonstrates how evolutionary forces optimize the fitness and host-adaptation of these pathogens. 相似文献
966.
Albert Calbet Andrey F. Sazhin Jens C. Nejstgaard Stella A. Berger Zachary S. Tait Lorena Olmos Despoina Sousoni Stamatina Isari Rodrigo A. Martínez Jean-Marie Bouquet Eric M. Thompson Ulf B?mstedt Hans H. Jakobsen 《PloS one》2014,9(4)
We studied the effects of future climate change scenarios on plankton communities of a Norwegian fjord using a mesocosm approach. After the spring bloom, natural plankton were enclosed and treated in duplicates with inorganic nutrients elevated to pre-bloom conditions (N, P, Si; eutrophication), lowering of 0.4 pH units (acidification), and rising 3°C temperature (warming). All nutrient-amended treatments resulted in phytoplankton blooms dominated by chain-forming diatoms, and reached 13–16 μg chlorophyll (chl) a l−1. In the control mesocosms, chl a remained below 1 μg l−1. Acidification and warming had contrasting effects on the phenology and bloom-dynamics of autotrophic and heterotrophic microplankton. Bacillariophyceae, prymnesiophyceae, cryptophyta, and Protoperidinium spp. peaked earlier at higher temperature and lower pH. Chlorophyta showed lower peak abundances with acidification, but higher peak abundances with increased temperature. The peak magnitude of autotrophic dinophyceae and ciliates was, on the other hand, lowered with combined warming and acidification. Over time, the plankton communities shifted from autotrophic phytoplankton blooms to a more heterotrophic system in all mesocosms, especially in the control unaltered mesocosms. The development of mass balance and proportion of heterotrophic/autotrophic biomass predict a shift towards a more autotrophic community and less-efficient food web transfer when temperature, nutrients and acidification are combined in a future climate-change scenario. We suggest that this result may be related to a lower food quality for microzooplankton under acidification and warming scenarios and to an increase of catabolic processes compared to anabolic ones at higher temperatures. 相似文献
967.
968.
Daniel Gianola Kent A. Weigel Nicole Kr?mer Alessandra Stella Chris-Carolin Sch?n 《PloS one》2014,9(4)
We examined whether or not the predictive ability of genomic best linear unbiased prediction (GBLUP) could be improved via a resampling method used in machine learning: bootstrap aggregating sampling (“bagging”). In theory, bagging can be useful when the predictor has large variance or when the number of markers is much larger than sample size, preventing effective regularization. After presenting a brief review of GBLUP, bagging was adapted to the context of GBLUP, both at the level of the genetic signal and of marker effects. The performance of bagging was evaluated with four simulated case studies including known or unknown quantitative trait loci, and an application was made to real data on grain yield in wheat planted in four environments. A metric aimed to quantify candidate-specific cross-validation uncertainty was proposed and assessed; as expected, model derived theoretical reliabilities bore no relationship with cross-validation accuracy. It was found that bagging can ameliorate predictive performance of GBLUP and make it more robust against over-fitting. Seemingly, 25–50 bootstrap samples was enough to attain reasonable predictions as well as stable measures of individual predictive mean squared errors. 相似文献
969.
Stella De Nicola Paola Dongiovanni Alessio Aghemo Cristina Cheroni Roberta D'Ambrosio Michele Pedrazzini Francesco Marabita Lorena Donnici Marco Maggioni Silvia Fargion Massimo Colombo Raffaele De Francesco Luca Valenti 《PloS one》2014,9(8)
Background and Aims
The PNPLA3 I148M sequence variant favors hepatic lipid accumulation and confers susceptibility to hepatic fibrosis and hepatocellular carcinoma. The aim of this study was to estimate the effect size of homozygosity for the PNPLA3 I148M variant (148M/M) on the fibrosis progression rate (FPR) and the interaction with age at infection in chronic hepatitis C (CHC).Methods
FPR was estimated in a prospective cohort of 247 CHC patients without alcohol intake and diabetes, with careful estimation of age at infection and determination of fibrosis stage by Ishak score.Results
Older age at infection was the strongest determinant of FPR (p<0.0001). PNPLA3 148M/M was associated with faster FPR in individuals infected at older age (above the median, 21 years; −0.64±0.2, n = 8 vs. −0.95±0.3, n = 166 log10 FPR respectively; p = 0.001; confirmed for lower age thresholds, p<0.05), but not in those infected at younger age (p = ns). The negative impact of PNPLA3 148M/M on fibrosis progression was more marked in subjects at risk of altered hepatic lipid metabolism (those with grade 2–3 steatosis, genotype 3, and overweight; p<0.05). At multivariate analysis, PNPLA3 148M/M was associated with FPR (incremental effect 0.08±0.03 log10 fibrosis unit per year; p = 0.022), independently of several confounders, and there was a significant interaction between 148M/M and older age at infection (p = 0.025). The association between 148M/M and FPR remained significant even after adjustment for steatosis severity (p = 0.032).Conclusions
We observed an interaction between homozygosity for the PNPLA3 I148M variant and age at infection in determining fibrosis progression in CHC patients. 相似文献970.
Stella M. Bernardo Christopher P. Allen Anna Waller Susan M. Young Tudor Oprea Larry A. Sklar Samuel A. Lee 《PloS one》2014,9(10)
Although three major classes of systemic antifungal agents are clinically available, each is characterized by important limitations. Thus, there has been considerable ongoing effort to develop novel and repurposed agents for the therapy of invasive fungal infections. In an effort to address these needs, we developed a novel high-throughput, multiplexed screening method that utilizes small molecules to probe candidate drug targets in the opportunistic fungal pathogen Candida albicans. This method is amenable to high-throughput automated screening and is based upon detection of changes in GFP levels of individually tagged target proteins. We first selected four GFP-tagged membrane-bound proteins associated with virulence or antifungal drug resistance in C. albicans. We demonstrated proof-of-principle that modulation of fluorescence intensity can be used to assay the expression of specific GFP-tagged target proteins to inhibitors (and inducers), and this change is measurable within the HyperCyt automated flow cytometry sampling system. Next, we generated a multiplex of differentially color-coded C. albicans strains bearing C-terminal GFP-tags of each gene encoding candidate drug targets incubated in the presence of small molecules from the Prestwick Chemical Library in 384-well microtiter plate format. Following incubation, cells were sampled through the HyperCyt system and modulation of protein levels, as indicated by changes in GFP-levels of each strain, was used to identify compounds of interest. The hit rate for both inducers and inhibitors identified in the primary screen did not exceed 1% of the total number of compounds in the small-molecule library that was probed, as would be expected from a robust target-specific, high-throughput screening campaign. Secondary assays for virulence characteristics based on null mutant strains were then used to further validate specificity. In all, this study presents a method for the identification and verification of new antifungal drugs targeted to fungal virulence proteins using C. albicans as a model fungal pathogen. 相似文献