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961.
Regulation of melanosome movement in the cell cycle by reversible association with myosin V. 总被引:6,自引:0,他引:6
S L Rogers R L Karcher J T Roland A A Minin W Steffen V I Gelfand 《The Journal of cell biology》1999,146(6):1265-1276
Previously, we have shown that melanosomes of Xenopus laevis melanophores are transported along both microtubules and actin filaments in a coordinated manner, and that myosin V is bound to purified melanosomes (Rogers, S., and V.I. Gelfand. 1998. Curr. Biol. 8:161-164). In the present study, we have demonstrated that myosin V is the actin-based motor responsible for melanosome transport. To examine whether myosin V was regulated in a cell cycle-dependent manner, purified melanosomes were treated with interphase- or metaphase-arrested Xenopus egg extracts and assayed for in vitro motility along Nitella actin filaments. Motility of organelles treated with mitotic extract was found to decrease dramatically, as compared with untreated or interphase extract-treated melanosomes. This mitotic inhibition of motility correlated with the dissociation of myosin V from melanosomes, but the activity of soluble motor remained unaffected. Furthermore, we find that myosin V heavy chain is highly phosphorylated in metaphase extracts versus interphase extracts. We conclude that organelle transport by myosin V is controlled by a cell cycle-regulated association of this motor to organelles, and that this binding is likely regulated by phosphorylation of myosin V during mitosis. 相似文献
962.
H.?BleckmannEmail author H.?Schmitz G.?von der?Emde 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2004,190(12):971-981
Nature has developed a stunning diversity of sensory systems. Humans and many animals mainly rely on visual information. In addition, they may use acoustic, olfactory, and tactile cues for object detection and spatial orientation. Beyond these sensory systems a large variety of highly specialized sensors have evolved. For instance, some buprestid beetles use infrared organs for the detection of forest fires. The infrared sensors of boid and crotalid snakes are used for prey detection at night. For object detection and spatial orientation many species of nocturnal fish employ active electrolocation. This review describes certain aspects of the detection and processing of infrared and electrosensory information. We show that the study of natural exotic sensory systems can lead to discoveries that are useful for the construction of technical sensors and artificial control systems. Comparative studies of animal sensory systems have the power to uncover at least a small fraction of the gigantic untapped reservoir of natural solutions for perceptive problems. 相似文献
963.
Martin Wilmking Tanja G. M. Sanders Yongxiang Zhang Saskia Kenter Steffen Holzk?mper Peter D. Crittenden 《Ecosystems》2012,15(7):1053-1064
Treeline advance alters albedo and carbon storage and is an important feedback mechanism to the global climate system. Establishment of trees north of the treeline requires favorable climate, suitable microsites, and viable seeds. Here we studied the influence of climate and microsite conditions on tree and seedling growth at four transects from forest through woodland to tundra in NW Russia, and tested the viability of seeds from the region. General growth patterns and establishment periods of the treeline species Picea obovata are similar across the study sites suggesting a regional driver (for example, climate). Individuals established as early as the 1640s, but mainly between 1850 and 1880, and during a major and continental scale establishment wave in the 1950s and 1960s. No establishment occurred after 1982. Older trees mainly showed significant and stable positive relationships to growing year summer temperatures and significant stable negative correlations to previous year summer temperatures in nearly all plots. Trees from the last establishment wave showed more mixed responses, but current year summer temperature positively affected growth. Active layer depth was similar in all plots with trees but decreased sharply in treeless tundra. A major role for the lack of recent establishment seems to be very low seed viability, possibly combined with early strong fall frosts, which might have severely limited successful recruitment in the last decades of the twentieth century. For a successful establishment of P. obovata in tundra areas of NW Russia, permafrost degradation and (generally) warmer winters might be a prerequisite. 相似文献
964.
A Schmitz K H Gartemann J Fiedler E Grund R Eichenlaub 《Applied and environmental microbiology》1992,58(12):4068-4071
Strains of Arthrobacter catalyze a hydrolytic dehalogenation of 4-chlorobenzoate (4-CBA) to p-hydroxybenzoate. The reaction requires ATP and coenzyme A (CoA), indicating activation of the substrate via a thioester, like that reported for Pseudomonas sp. strain CBS3 (J. D. Scholten, K.-H. Chang, P. C. Babbit, H. Charest, M. Sylvestre, and D. Dunaway-Mariano, Science 253:182-185, 1991). The dehalogenase genes of Arthrobacter sp. strain SU were cloned and expressed in Escherichia coli. Analyses of deletions indicate that dehalogenation depends on three open reading frames (ORFs) which are organized in an operon. There is extensive sequence homology to corresponding gene products in Pseudomonas sp. strain CBS3, suggesting that ORF1 and ORF2 encode a 4-CBA-CoA-ligase and a 4-CBA-CoA dehalogenase, respectively. ORF3 possibly represents a thioesterase, although no homology to the enzyme from Pseudomonas sp. strain CBS3 exists. 相似文献
965.
Lauchlan H. Fraser William L. Harrower Heath W. Garris Scott Davidson Paul D. N. Hebert Rick Howie Anne Moody David Polster Oswald J. Schmitz Anthony R. E. Sinclair Brian M. Starzomski Thomas P. Sullivan Roy Turkington Dennis Wilson 《Restoration Ecology》2015,23(5):503-507
Ecological restoration projects have traditionally focused on vegetation as both a means (seeding, planting, and substrate amendments) and ends (success based upon primary productivity and vegetation diversity). This vegetation‐centric approach to ecological restoration stems from an historic emphasis on esthetics and cost but provides a limited measure of total ecosystem functioning and overlooks alternative ways to achieve current and future restoration targets. We advocate a shift to planning beyond the plant community and toward the physical and biological components necessary to initiate autogenic recovery, then guiding this process through the timely introduction of top predators and environmental modifications such as soil amendments and physical structures for animal nesting and refugia. 相似文献
966.
Lindner SN Vidaurre D Willbold S Schoberth SM Wendisch VF 《Applied and environmental microbiology》2007,73(15):5026-5033
Corynebacterium glutamicum is able to accumulate up to 600 mM cytosolic phosphorus in the form of polyphosphate (poly P). Granular poly P (volutin) can make up to 37% of the internal cell volume. This bacterium lacks the classic enzyme of poly P synthesis, class I polyphosphate kinase (PPK1), but it possesses two genes, ppk2A (corresponds to NCgl0880) and ppk2B (corresponds to NCgl2620), for putative class II (PPK2) PPKs. Deletion of ppk2B decreased PPK activity and cellular poly P content, while overexpression of ppk2B increased both PPK activity and cellular poly P content. Neither deletion nor overexpression of ppk2A changed specific activity of PPK or cellular poly P content significantly. Purified PPK2B of C. glutamicum is active as a homotetramer and formed poly P with an average chain length of about 125, as determined with (31)P nuclear magnetic resonance. The catalytic efficiency of C. glutamicum PPK2B was higher in the poly P-forming direction than for nucleoside triphosphate formation from poly P. The ppk2B deletion mutant, which accumulated very little poly P and grew as C. glutamicum wild type under phosphate-sufficient conditions, showed a growth defect under phosphate-limiting conditions. 相似文献
967.
Synthesis and Characterization of High‐Energy,High‐Power Spinel‐Layered Composite Cathode Materials for Lithium‐Ion Batteries 下载免费PDF全文
Steffen Krueger Vassilios Siozios Jie Li Sascha Nowak Martin Winter 《Liver Transplantation》2015,5(5)
Spinel‐layered composites, where a high‐voltage spinel is incorporated in a layered lithium‐rich (Li‐rich) cathode material with a nominal composition x{0.6Li2MnO3 · 0.4[LiCo0.333Mn0.333Ni0.333]O2} · (1 – x) Li[Ni0.5Mn1.5]O4 (x = 0, 0.3, 0.5, 0.7, 1) are synthesized via a hydroxide assisted coprecipitation route to generate high‐energy, high‐power cathode materials for Li‐ion batteries. X‐ray diffraction patterns and the cyclic voltammetry investigations confirm the presence of both the parent components in the composites. The electrochemical investigations performed within a wide potential window show an increased structural stability of the spinel component when incorporated into the composite environment. All the composite materials exhibit initial discharge capacities >200 mAh g–1. The compositions with x = 0.5 and 0.7 show excellent cycling stability among the investigated materials. Moreover, the first cycle Coulombic efficiency achieve a dramatic improvement with the incorporation of the spinel component. More notably, the composite materials with increased spinel component exhibit superior rate capability compared with the parent Li‐rich material especially together with the highest capacity retention for x = 0.5 composition, making this as the optimal high‐energy high‐power material. The mechanisms involved in the symbiotic relationship of the spinel and layered Li‐rich components in the above composites are discussed. 相似文献
968.
969.
970.
Keen Carl L. Holt Roberta R. Polagruto John A. Wang Janice F. Schmitz Harold H. 《Phytochemistry Reviews》2002,1(2):231-240
Fruits and vegetables have historically been considered rich sources of essential dietary micronutrients, soluble fiber, and
antioxidants. More recently they are have been recognized as important sources for a wide array of phytochemicals that individually,
or in combination, may benefit vascular health. Flavonoids are the largest, and most widely distributed class of phytochemicals,
and can be further subdivided into several different sub-classes. Several epidemiology studies have observed an inverse association
between flavonoid intake and risk of cardiovascular mortality. One sub-class of flavonoids, the flavanols, is found in foods
such as grapes, red wine, tea, cocoa and chocolate; however, it is important to note that common food processing practices
can significantly reduce the levels of these compounds found in finished food products. Recent studies have examined the potential
of flavanol-rich cocoa and chocolates to influence vascular health. In this review, we discuss evidence for the hypothesis
that the consumption of flavanol-rich cocoa can reduce the risk for cardiovascular disease through a multiplicity of mechanisms,
including changes in oxidant defense mechanisms, vascular reactivity, cytokine production, and platelet function.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献