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131.
Oberle S Abate A Grosser N Vreman HJ Dennery PA Schneider HT Stalleicken D Schröder H 《Biochemical and biophysical research communications》2002,290(5):1539-1544
The organic nitrate pentaerythrityl tetranitrate (PETN) is known to exert long-term antioxidant and antiatherogenic effects by as yet unidentified mechanisms. In cultured endothelial cells derived from human umbilical vein, the active PETN metabolite PETriN (0.01-1 mM) increased heme oxygenase (HO)-1 mRNA and protein levels in a concentration-dependent fashion. HO-1 induction was accompanied by a marked increase in catalytic activity of the enzyme as reflected by enhanced formation of carbon monoxide and bilirubin. Pretreatment with PETriN or bilirubin at low micromolar concentrations protected endothelial cells from hydrogen peroxide-mediated toxicity. HO-1 induction and endothelial protection by PETriN were not mimicked by isosorbide dinitrate, another long-acting nitrate. The present study demonstrates that PETriN stimulates mRNA and protein expression as well as enzymatic activity of the antioxidant defense protein HO-1 in endothelial cells. Increased HO-1 expression and ensuing formation of cytoprotective bilirubin may contribute to and explain the specific antioxidant and antiatherogenic actions of PETN. 相似文献
132.
Marx S Baumgärtner M Kannan S Braun HP Lang BF Burger G Kunnan S 《Molecular biology and evolution》2003,20(1):145-153
In eubacteria, the respiratory bc(1) complex (complex III) consists of three or four different subunits, whereas that of mitochondria, which have descended from an alpha-proteobacterial endosymbiont, contains about seven additional subunits. To understand better how mitochondrial protein complexes evolved from their simpler bacterial predecessors, we purified complex III of Seculamonas ecuadoriensis, a member of the jakobid protists, which possess the most bacteria-like mitochondrial genomes known. The S. ecuadoriensis complex III has an apparent molecular mass of 460 kDa and exhibits antimycin-sensitive quinol:cytochrome c oxidoreductase activity. It is composed of at least eight subunits between 6 and 46 kDa in size, including two large "core" subunits and the three "respiratory" subunits. The molecular mass of the S. ecuadoriensis bc(1) complex is slightly lower than that reported for other eukaryotes, but about 2x as large as complex III in bacteria. This indicates that the departure from the small bacteria-like complex III took place at an early stage in mitochondrial evolution, prior to the divergence of jakobids. We posit that the recruitment of additional subunits in mitochondrial respiratory complexes is a consequence of the migration of originally alpha-proteobacterial genes to the nucleus. 相似文献
133.
Proteome analysis. Novel proteins identified at the peribacteroid membrane from Lotus japonicus root nodules 总被引:7,自引:0,他引:7
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The peribacteroid membrane (PBM) forms the structural and functional interface between the legume plant and the rhizobia. The model legume Lotus japonicus was chosen to study the proteins present at the PBM by proteome analysis. PBM was purified from root nodules by an aqueous polymer two-phase system. Extracted proteins were subjected to a global trypsin digest. The peptides were separated by nanoscale liquid chromatography and analyzed by tandem mass spectrometry. Searching the nonredundant protein database and the green plant expressed sequence tag database using the tandem mass spectrometry data identified approximately 94 proteins, a number far exceeding the number of proteins reported for the PBM hitherto. In particular, a number of membrane proteins like transporters for sugars and sulfate; endomembrane-associated proteins such as GTP-binding proteins and vesicle receptors; and proteins involved in signaling, for example, receptor kinases, calmodulin, 14-3-3 proteins, and pathogen response-related proteins, including a so-called HIR protein, were detected. Several ATPases and aquaporins were present, indicating a more complex situation than previously thought. In addition, the unexpected presence of a number of proteins known to be located in other compartments was observed. Two characteristic protein complexes obtained from native gel electrophoresis of total PBM proteins were also analyzed. Together, the results identified specific proteins at the PBM involved in important physiological processes and localized proteins known from nodule-specific expressed sequence tag databases to the PBM. 相似文献
134.
Arzumanov A Stetsenko DA Malakhov AD Reichelt S Sørensen MD Babu BR Wengel J Gait MJ 《Oligonucleotides》2003,13(6):435-453
135.
Heinz C Karosi S Niederweis M 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2003,790(1-2):337-348
MspA is the prototype of a new family of tetrameric porins and provides the main general diffusion pathway for hydrophilic compounds through the outer membrane of Mycobacterium smegmatis. Structural analysis was hampered by the scarce amount of pure protein. After replacement of the GC-rich codons of the mspA gene by codons optimal for high-level expression in Escherichia coli, the mature MspA protein was overproduced in E. coli. The recombinant MspA (rMspA) monomer (M(r) 20000) was purified by anion exchange and hydrophobic interaction chromatography yielding 2.6 mg pure protein per liter of culture. This exceeded the yield of the native protein 10-fold. Circular dichroism revealed that rMspA is folded in a native-like structure. rMspA assembled partially to the channel-forming tetramer both during expression in E. coli and after purification in vitro. Thus, overexpression in E. coli and chromatographic purification are key steps towards a high resolution structure of MspA. 相似文献
136.
Stöcker M Tur MK Sasse S Krüssmann A Barth S Engert A 《Protein expression and purification》2003,28(2):211-219
Immunotoxins consist of a target-cell-specific binding moiety, chemically or recombinantly linked to a cytotoxic component. A number of different immunotoxins (IT) have increasingly been evaluated for immunotherapy. Since these foreign proteins are highly immunogenic in human, we have developed recombinant IT using the human ribonuclease angiogenin. Due to their potential toxic effects on eucaryotic cells, these IT are usually expressed in bacteria. Depending on the structure, size, and sequence of the desired IT, bacterial expression can be limited and the yield after purification be unsatisfactory. Therefore, the expression of IT in eucaryotic cells could provide a promising alternative. For this purpose we genetically fused the anti-CD30 single-chain variable fragment (scFv) Ki4 to the N- and C-termini of recombinant angiogenin. Both IT possess leader sequences, which mediate their secretion into the cell culture supernatant. Using a bicistronic mRNA the IT were simultaneously expressed together with enhanced green fluorescent protein (EGFP). This allows direct monitoring of transfected cells. An additional plasmid encoded Zeocin resistance enhances the cultivation of transfected cells under selection pressure. Three days after transfection of 293T-cells, unpurified IT were analyzed by flow cytometry and competitive cell proliferation assays. This is the first report on the use of eucaryotic cells for the secretion of functionally active IT with a human effector domain. 相似文献
137.
138.
Impaired insulin secretion and glucose tolerance in beta cell-selective Ca(v)1.2 Ca2+ channel null mice
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Schulla V Renström E Feil R Feil S Franklin I Gjinovci A Jing XJ Laux D Lundquist I Magnuson MA Obermüller S Olofsson CS Salehi A Wendt A Klugbauer N Wollheim CB Rorsman P Hofmann F 《The EMBO journal》2003,22(15):3844-3854
Insulin is secreted from pancreatic beta cells in response to an elevation of cytoplasmic Ca(2+) resulting from enhanced Ca(2+) influx through voltage-gated Ca(2+) channels. Mouse beta cells express several types of Ca(2+) channel (L-, R- and possibly P/Q-type). beta cell-selective ablation of the gene encoding the L-type Ca(2+) channel subtype Ca(v)1.2 (betaCa(v)1.2(-/-) mouse) decreased the whole-cell Ca(2+) current by only approximately 45%, but almost abolished first-phase insulin secretion and resulted in systemic glucose intolerance. These effects did not correlate with any major effects on intracellular Ca(2+) handling and glucose-induced electrical activity. However, high-resolution capacitance measurements of exocytosis in single beta cells revealed that the loss of first-phase insulin secretion in the betaCa(v)1.2(-/-) mouse was associated with the disappearance of a rapid component of exocytosis reflecting fusion of secretory granules physically attached to the Ca(v)1.2 channel. Thus, the conduit of Ca(2+) entry determines the ability of the cation to elicit secretion. 相似文献
139.
140.
Stefanie?HellwegEmail author Thomas?B.?Hofstetter Konrad?Hungerbuhler 《The International Journal of Life Cycle Assessment》2003,8(1):8-18
Background In Life-Cycle Assessment (LCA), decision makers are often faced with tradeoffs between current and future impacts. One typical
example is waste incineration, where immediate emissions to the air from the incineration process have to be weighted against
future emissions of slag landfills. Long-term impacts are either completely taken into account or they are entirely disregarded
in case of a temporal cut-off. Temporal cutoffs are a special case of discounting.
Objective In this paper, discounting is defined as valuing damages differently at different points of time using a positive or negative
discount rate. Apart from temporal cut-offs, discounting has rarely been applied in LCA so far. It is the goal of this paper
to discuss the concept of discounting and its applicability in the context of LCA.
Methods For this purpose, we first review the arguments for discounting and its principles in economic sciences. Discounting in economics
can be motivated by pure time preference, productivity of capital, diminishing marginal utility of consumption, and uncertainties.
The nominal discount rate additionally includes changes in the price level. These arguments and their justification are discussed
in the context of environmental impacts harming future generations.
Results and Discussion It is concluded that discounting across generations because of pure time preference contradicts fundamental ethical values
and should therefore not be applied in LCA. However, it has to be acknowledged that in practice decision makers often use
positive discount rates because of pure time preference — either because they might profit from imposing environmental damage
on others instead of themselves or because people in the far future are not of immediate concern to them. Discounting because
of the productivity of capital assumes a relationship between monetary values and environmental impact. If such a relationship
is accepted, discounting could be applied. However, future generations should be compensated for the environmental damage.
It is likely that they would demand a higher compensation if the real per capita income increases. As both the compensation
and the discount rate are related to economic growth, the overall discount rate might be close to zero. It is shown that the
overall discount rate might even be negative considering that the required compensation could increase (even to infinite)
if natural assets remain scarce, whereas the utility of consumption diminishes with increasing income. Uncertainties could
justify both positive and negative discount rates. Since the relationship between uncertainties and the magnitude of damage
is generally not exponential, we recommend to model changes in the magnitude of damage in scenario analysis instead of considering
it in discounting (which requires an exponential function of time in the case of a constant discount rate). We investigated
the influence of discounting in a case study of heavy metal emissions from slag landfills. It could be shown that even small
discount rates of less than 1 % lead to a significant reduction of the impact score, whereas negative discount rates inflate
the results.
Conclusions and Recommendations Discounting is only applicable when temporally differentiated data is available. In some cases, such a temporal differentiation
is necessary to take sound decisions, especially when long emission periods are involved. An example is the disposal of nuclear
or heavy metal-containing waste. In these cases, the results might completely depend on the discount rate. This paper helps
to structure arguments and thus to support the decision about whether or not discounting should be applied in an LCA. 相似文献