全文获取类型
收费全文 | 461篇 |
免费 | 35篇 |
国内免费 | 1篇 |
专业分类
497篇 |
出版年
2021年 | 6篇 |
2020年 | 2篇 |
2019年 | 9篇 |
2018年 | 9篇 |
2017年 | 7篇 |
2016年 | 13篇 |
2015年 | 8篇 |
2014年 | 13篇 |
2013年 | 25篇 |
2012年 | 31篇 |
2011年 | 31篇 |
2010年 | 14篇 |
2009年 | 13篇 |
2008年 | 27篇 |
2007年 | 18篇 |
2006年 | 19篇 |
2005年 | 19篇 |
2004年 | 18篇 |
2003年 | 9篇 |
2002年 | 12篇 |
2001年 | 11篇 |
2000年 | 14篇 |
1999年 | 11篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 7篇 |
1993年 | 4篇 |
1992年 | 12篇 |
1991年 | 12篇 |
1990年 | 10篇 |
1989年 | 9篇 |
1988年 | 5篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 6篇 |
1982年 | 6篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1974年 | 8篇 |
1973年 | 4篇 |
1971年 | 7篇 |
1970年 | 6篇 |
1969年 | 1篇 |
1967年 | 3篇 |
排序方式: 共有497条查询结果,搜索用时 15 毫秒
491.
492.
Dietmar Lohmann Bernhard Horsthemke Gabriele Gillessen-Kaesbach Fritz Heinrich Stefani Heinz Höfler 《Human genetics》1992,89(1):49-53
Summary Loss of function of both copies of the RB1 gene is a causal event in the development of retinoblastoma. The predisposition to this tumor can be inherited as an autosomal dominant trait. Direct detection of the genetic defect is important for presymptomatic DNA diagnosis and genetic counseling in families with hereditary retinoblastoma. We have used multiplex polymerase chain reaction and high-resolution polyacrylamide gel electrophoresis to detect RB1 gene deletions as small as one base pair. By using three independent sets of amplification reactions, which cover 26% of the RB1 gene coding region, we identified RB1 gene deletions in the DNA of peripheral blood cells in 3 out of 24 (12.5%) unrelated patients with hereditary retinoblastoma. In one case, formalin-fixed paraffin-embedded tumor material was also used to detect the mutation. Sequencing of the mutated alleles revealed deletions of 1, 3 and 10 base pairs. Each deleted region was flanked by direct repeats. 相似文献
493.
494.
Massimo Stefani Alessandra Modesti Guido Camici Giampaolo Manao Gianni Cappugi Andrea Berti Giampietro Ramponi 《Journal of Protein Chemistry》1986,5(5):307-321
The complete amino acid sequence of duck skeletal muscle acylphosphatase is presented. The sequence was studied by the manual Edman degradation of the complete series of tryptic peptides and the amino acid composition of peptic peptides. The NH2-terminus is acetylated, and the polypeptide consists of 102 amino acid residues. The sequence is compared with other known acylphosphatases from the skeletal muscle of several vertebrate species. 相似文献
495.
A. L. Rodriguez F. S. Stefani C. E. de Oliveira Praes¶ A. Piaceski¶ M. P. Oliveira† P. Martins† V. D. da Silva‡ C. Bonorino§ M. E. Bauer§ 《Cell proliferation》2009,42(4):562-567
Due to an increasing number of skin diseases as a result of exposure to ultraviolet (UV) radiation, it is necessary to evaluate the effectiveness of new skin care formulations with broad-spectrum sunscreens.
Objectives: This study aims to assess the status of nerve fibres in healthy human skin, to quantify effects of UV radiation on nerve endings, and to evaluate neuroprotective effects of new skin care formulations against UV exposure damage.
Methods: Samples were obtained from 34 female patients enrolled for plastic surgery and were immediately treated (10 min) with three emulsions: Cream 1, Cream 2 (placebo) and a sunscreen with sun protection factor 15 (SPF15). Control samples and those treated with the cream emulsions were exposed to UVA and UVB for 60 min. Nerve fibres were identified by immunofluorescence using a monoclonal antibody (anti-human CD56/NCAM). Cell damage was assessed by image analysis.
Results: Several cellular nervous structures were identified in the skin samples, including free nerve endings. UVA and UVB significantly decreased (40–60%) density of nerve endings in the control samples and those treated with placebo (Cream 2) or SPF15 (all P < 0.001). Cream 1 completely blocked effects of UV radiation on nerve endings ( P > 0.05 vs. control).
Conclusions: Quantification of cell damage induced by UV radiation provides useful information for identification of new skin care compounds with neuroprotective properties. 相似文献
Objectives: This study aims to assess the status of nerve fibres in healthy human skin, to quantify effects of UV radiation on nerve endings, and to evaluate neuroprotective effects of new skin care formulations against UV exposure damage.
Methods: Samples were obtained from 34 female patients enrolled for plastic surgery and were immediately treated (10 min) with three emulsions: Cream 1, Cream 2 (placebo) and a sunscreen with sun protection factor 15 (SPF15). Control samples and those treated with the cream emulsions were exposed to UVA and UVB for 60 min. Nerve fibres were identified by immunofluorescence using a monoclonal antibody (anti-human CD56/NCAM). Cell damage was assessed by image analysis.
Results: Several cellular nervous structures were identified in the skin samples, including free nerve endings. UVA and UVB significantly decreased (40–60%) density of nerve endings in the control samples and those treated with placebo (Cream 2) or SPF15 (all P < 0.001). Cream 1 completely blocked effects of UV radiation on nerve endings ( P > 0.05 vs. control).
Conclusions: Quantification of cell damage induced by UV radiation provides useful information for identification of new skin care compounds with neuroprotective properties. 相似文献
496.
Cytochrome c oxidase (complex IV of the respiratory chain) was studied histochemically in autoptic human extraocular muscles (n = 135), revealing randomly distributed single fibers without enzyme activity. The enzyme defect was expressed in all the mitochondria of an involved fiber as evidenced by ultracytochemistry. Succinate dehydrogenase showed normal histochemical reactivity. The defects occurred already in the second decade and were regularly seen from the third decade on. The defect density (defects/mm2) increased from approx. 1/mm2 below the fifth decade to about 4/mm2 in advanced age (P = 0.000). The highest defect density was observed in the levator palpebrae muscle. On the whole, the defect density was about 5-6 times higher in the extraocular muscles than in the limb muscle, diaphragm and heart (Müller-H?cker, 1989, 1990). Immunocytochemical detection of cytochrome c oxidase showed that loss of cytochrome c oxidase activity was due to an almost complete absence of both nuclear and mitochondria subunits of the enzyme. The results document different organ and heterogenic cellular sensitivity to the age-related loss of cytochrome c oxidase. The loss of both mitochondrial and nuclear subunits indicates that nuclear factors are most probably involved in the decline of the respiratory chain function in senescence. 相似文献
497.
Lysosomal sialidase deficiency: increased ganglioside content in autopsy tissues of a sialidosis patient 总被引:3,自引:0,他引:3
Organs obtained at autopsy from a patient with sialidosis were analyzed for 'bound' sialic acid and their ganglioside and neutral glycolipid patterns determined. The water-soluble bound sialic acid was increased between 10- and 17-fold in visceral organs, but only about 2-fold in the brain, when compared to normal controls. Lipid-bound sialic acid was increased up to 8-fold in visceral organs due to elevated amounts of gangliosides GM3, GD3 and probably GM4 and LM1, whereas the brain showed no deviation from controls. An alteration of the neutral glycolipid pattern was also observed. The results indicate an impaired catabolism of gangliosides in sialidosis in addition to that of sialyloligosaccharides and sialoglycoproteins. 相似文献