全文获取类型
收费全文 | 145篇 |
免费 | 21篇 |
出版年
2022年 | 1篇 |
2021年 | 4篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 5篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2012年 | 6篇 |
2011年 | 3篇 |
2010年 | 3篇 |
2009年 | 9篇 |
2008年 | 7篇 |
2007年 | 9篇 |
2006年 | 11篇 |
2005年 | 9篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1987年 | 8篇 |
1986年 | 6篇 |
1985年 | 3篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 1篇 |
1973年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1939年 | 1篇 |
排序方式: 共有166条查询结果,搜索用时 15 毫秒
141.
142.
143.
Data management and preliminary data analysis in the pilot phase of the HUPO Plasma Proteome Project
Adamski M Blackwell T Menon R Martens L Hermjakob H Taylor C Omenn GS States DJ 《Proteomics》2005,5(13):3246-3261
The pilot phase of the HUPO Plasma Proteome Project (PPP) is an international collaboration to catalog the protein composition of human blood plasma and serum by analyzing standardized aliquots of reference serum and plasma specimens using a variety of experimental techniques. Data management for this project included collection, integration, analysis, and dissemination of findings from participating organizations world-wide. Accomplishing this task required a communication and coordination infrastructure specific enough to support meaningful integration of results from all participants, but flexible enough to react to changing requirements and new insights gained during the course of the project and to allow participants with varying informatics capabilities to contribute. Challenges included integrating heterogeneous data, reducing redundant information to minimal identification sets, and data annotation. Our data integration workflow assembles a minimal and representative set of protein identifications, which account for the contributed data. It accommodates incomplete concordance of results from different laboratories, ambiguity and redundancy in contributed identifications, and redundancy in the protein sequence databases. Recommendations of the PPP for future large-scale proteomics endeavors are described. 相似文献
144.
145.
Takusan: a large gene family that regulates synaptic activity 总被引:1,自引:0,他引:1
We have characterized a rodent-specific gene family designated alpha-takusan (meaning "many" in Japanese). We initially identified a member of the family whose expression is upregulated in mice lacking the NMDAR subunit NR3A. We then isolated cDNAs encoding 46 alpha-takusan variants from mouse brains. Most variants share an approximately 130 aa long sequence, which contains the previously identified domain of unknown function 622 (DUF622) and is predicted to form coiled-coil structures. Single-cell PCR analyses indicate that one neuron can express multiple alpha-takusan variants and particular variants may predominate in certain cell types. Forced expression in cultured hippocampal neurons of two variants, alpha1 or alpha2, which bind either directly or indirectly to PSD-95, leads to an increase in PSD-95 clustering, dendritic spine density, GluR1 surface expression, and AMPAR activity. Conversely, treating cultured neurons with RNAi targeting alpha-takusan variants resulted in the opposite phenotype. Hence, alpha-takusan represents a large gene family that regulates synaptic activity. 相似文献
146.
Motomi Ito Kuniaki Watanabe Yoko Kita Takayuki Kawahara DJ Crawford Tetsukazu Yahara 《Journal of plant research》2000,113(1):79-89
Eupatorium were examined by sequencing the internal transcribed spacers (ITS) of nuclear ribosomal DNA and restriction site analysis
of chloroplast DNA. Molecular data provided strong evidence that (1) this genus originated in North America, (2) the genus
diverged into three morphological species groups, Eutrochium, Traganthes and Uncasia in North America, and (3) one of the North American Uncasia lineages migrated into temperate Europe and eastern Asia over the Bering land bridge. The estimated divergence times support
a late Miocene to early Pliocene migration from North America to Eurasia via the Bering land bridge. A European species was
sister to all of the eastern Asian species examined. The disjunct distribution pattern of the genus Eupatorium is incongruent with the classical Arcto-Tertiary geoflora concept.
Received 13 September 1999/ Accepted in revised form 4 January 2000 相似文献
147.
Beatrice States Stanton Segal 《Biochemical and biophysical research communications》1982,105(4):1327-1332
Cysteamine when incubated with human normal and cystinotic fibroblasts produces significant alterations in cellular amino acid pools with a decrease in proline, glycine, histidine, and methionine as well as branch chain and dibasic amino acids. Serine content was increased while aspartate, glutamate and glutamine were unchanged. The uptake of L-proline by cysteamine treated cells was impaired suggesting that altered uptake of amino acids may be a factor in the cysteamine effect. 相似文献
148.
Differences between OK and LLC-PK1 cells: cystine handling 总被引:1,自引:0,他引:1
149.
Several studies have indicated that olfactory responses are impeded by
amiloride. Therefore, it was of interest to see whether, and if so which,
olfactory epithelial cellular compartments have amiloride- sensitive
structures. Using ultrastructural methods that involved rapid freezing,
freeze-substitution and low temperature embedding of olfactory epithelia,
this study shows that, in the rat, this tissue is immunoreactive to
antibodies against amiloride sensitive Na(+)- channels. However, microvilli
of olfactory supporting cells, as opposed to receptor cilia, contained most
of the immunoreactive sites. Apices from which the microvilli sprout and
receptor cell dendritic knobs had much less if any of the
amiloride-antibody binding sites. Using a direct ligand-binding
cytochemical method, this study also confirms earlier ones that showed that
olfactory receptor cell cilia have Na+, K(+)-ATPase. It is proposed that
supporting cell microvilli and the receptor cilia themselves have
mechanisms, different but likely complementary, that participate in
regulating the salt concentration around the receptor cell cilia. In this
way, both structures help to provide the ambient mucous environment for
receptor cells to function properly. This regulation of the salt
concentration of an ambient fluid environment is a function that the
olfactory epithelium shares with cells of transporting epithelia, such as
those of kidney.
相似文献
150.
Beatrice States Robert Reynolds Judithann Lee Stanton Segal 《In vitro cellular & developmental biology. Plant》1990,26(2):105-112
Summary Large numbers of kidney epithelial cells were cultured successfully from isolated dog proximal tubule segments. Cells in primary
culture and in first passage retained the cystine-dibasic amino acid co-transporter system which is found in vivo and in freshly
isolated proximal tubule segments. In contrast to other cultured cells, the cystine-glutamate anti-porter was absent in primary
cultures. However, this anti-porter system seemed to be developing in cells in first passage. The intracellular ratio of cysteine:reduced
glutathione (CSH:GSH) was maintained at 1∶36 in both primary cultures and in low passage cells. Incubation of cells in primary
culture for 5 min at 37°C with 0.025 mM [35S]l-cystine resulted in incorporation of approximately 36 and 8.5% of the label into intracellular CSH and GSH, respectively.
These cultured cells, therefore, seem to be an excellent model system for the eventual elucidation of a) the intricacies of
cystine metabolism and b) regulation of 1) the cystine-dibasic amino acid co-transporter system and 2) the development of
the cystine-glutamate anti-porter system.
Supported by National Institutes of Health, Bethesda, MD, grant no. DK40555 and The National Kidney Foundation of the Delaware
Valley. 相似文献