全文获取类型
收费全文 | 784787篇 |
免费 | 95455篇 |
国内免费 | 434篇 |
专业分类
880676篇 |
出版年
2016年 | 8551篇 |
2015年 | 13054篇 |
2014年 | 15043篇 |
2013年 | 20912篇 |
2012年 | 23876篇 |
2011年 | 23826篇 |
2010年 | 16152篇 |
2009年 | 15339篇 |
2008年 | 21888篇 |
2007年 | 22470篇 |
2006年 | 21059篇 |
2005年 | 20295篇 |
2004年 | 20139篇 |
2003年 | 19469篇 |
2002年 | 18880篇 |
2001年 | 34349篇 |
2000年 | 34772篇 |
1999年 | 28045篇 |
1998年 | 10764篇 |
1997年 | 11398篇 |
1996年 | 10959篇 |
1995年 | 10362篇 |
1994年 | 10219篇 |
1993年 | 10049篇 |
1992年 | 23625篇 |
1991年 | 22889篇 |
1990年 | 22520篇 |
1989年 | 21854篇 |
1988年 | 20118篇 |
1987年 | 19775篇 |
1986年 | 18170篇 |
1985年 | 18236篇 |
1984年 | 15163篇 |
1983年 | 13251篇 |
1982年 | 10595篇 |
1981年 | 9453篇 |
1980年 | 8981篇 |
1979年 | 14584篇 |
1978年 | 11736篇 |
1977年 | 10600篇 |
1976年 | 10014篇 |
1975年 | 10844篇 |
1974年 | 11664篇 |
1973年 | 11458篇 |
1972年 | 10258篇 |
1971年 | 9518篇 |
1970年 | 8130篇 |
1969年 | 7694篇 |
1968年 | 6869篇 |
1967年 | 6196篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
401.
Tubulin subunit carboxyl termini determine polymerization efficiency 总被引:21,自引:0,他引:21
Cleavage of tubulin by subtilisin removes a small (Mr less than 2000) fragment from the C-terminal end of both alpha and beta subunits. The resulting protein is much reduced in negative charge. The cleaved, less acidic protein retains its competence to polymerize in a GTP-dependent and cold-, GDP-, and podophyllotoxin-sensitive manner and assembles into sheets or bundles of twisted filaments. The critical concentration for polymerization of the cleaved protein is about 50-fold lower than that for intact tubulin. It is proposed that the C termini of the subunits normally impede polymerization. 相似文献
402.
Summary Net aerial primary production and accumulation of nutrients by the grasses and nongrasses of the ground layer community of a cerrado vegetation in central Brazil were determined in burnt and unburnt areas. The net aerial primary production of the ground layer community was 327 gm–2 in the unburnt area and only 242 gm–2 in the burnt area during the first year after fire. Grasses contributed 68 to 78% of the aerial biomass of the ground layer in the unburnt area. The live biomass in the burnt and unburnt areas was comparable by the end of the first dry period after the fire. The major part of N, P and K in the aerial biomass was in the grasses. The concentration of all nutrients in the aerial biomass was generally higher in the burnt area during the first year after the fire. 相似文献
403.
Structural localization of the sequence alpha 235-242 of the nicotinic acetylcholine receptor 总被引:1,自引:0,他引:1
M Criado V Sarin J L Fox J Lindstrom 《Biochemical and biophysical research communications》1985,128(2):864-871
Two monoclonal antibodies (mAb 254 and 255) were obtained against a synthetic peptide corresponding to the sequence 235-242 of the alpha-subunit of Torpedo acetylcholine receptor. These mAbs could bind to receptor in native membrane vesicles only when these vesicles were permeabilized, suggesting that the sequence alpha 235-242 is exposed on the cytoplasmic surface of the receptor. Further evidence for the cytoplasmic localization of this sequence was partial competition for binding between these mAbs and mAbs previously demonstrated to bind to the cytoplasmic part of the receptor. A model is proposed which accounts for all the experimental data obtained thus far on the transmembrane orientation of the subunit polypeptide chains. 相似文献
404.
The dynamics of leucocytes in abdominal abscesses were studied using indium-111 autologous leucocyte scanning in 30 patients. Thirteen patients showing enteric drainage of leucocytes on delayed scans were characterised by a lack of abdominal localising signs and a low detection rate by ultrasound (25%). By contrast, 16 of 17 patients without enteric drainage had abdominal signs, and in these patients ultrasound was associated with a higher detection rate (58%). Despite the presence of an enteric route of drainage for the abscess 10 of the 13 patients needed surgical intervention. These results help explain the wide variation in clinical presentation of abdominal abscesses; suggest that 111In leucocyte scanning should be the initial investigation in those patients without focal signs; and show that formal surgical drainage is needed in patients recognised as having enteric communication with abscesses. 相似文献
405.
Occurrence of multiple-antibiotic-resistant enteric bacteria in domestic sewage and oxidation lagoons 总被引:1,自引:0,他引:1
The coliform bacterial population in the Grand Forks, N.Dak. sewage system was examined for multiple-antibiotic-resistant organisms over a 1-year period. Multiple-antibiotic-resistant coliforms were found to be common in the sewage, and their numbers remained fairly constant relative to the total coliform population throughout the year. Resistance to kanamycin, tetracycline, and ampicillin was found to be transferable at variable rates. Transfer rates were found to be temperature sensitive and were optimal at 35 degrees C. Although 75% of the multiple-antibiotic-resistant coliforms were capable of transferring resistance at some level, only 25% were capable of transferring resistance at rates greater than 10(-3) transconjugants per initial donor. 相似文献
406.
407.
408.
409.
J Markussen I Diers A Engesgaard M T Hansen P Hougaard L Langkjaer K Norris U Ribel A R S?rensen E S?rensen 《Protein engineering》1987,1(3):215-223
It has previously been found that insulins, to which positive charge has been added by substitutions in position B30, thus raising the isoelectric point towards pH 7, had a prolonged action when injected as slightly acidic solutions because such derivatives crystallize very readily upon neutralization. Positive charge has now been added by substituting the B13 and A17 glutamic acid residues with glutamines and B27 threonine with lysine or arginine. These substitutions were introduced by site-specific mutagenesis in a gene coding for a single-chain insulin precursor. By tryptic transpeptidation the single-chain precursors were transformed to the double-chain insulin structure, concomitantly with incorporation of residue B30. Thus insulins combining B13 glutamine, A17 glutamine and B27 lysine or arginine with B30 threonine, threonine amide or lysine amide were synthesized. The time course of blood glucose lowering effect and the absorption were studied after subcutaneous injection in rabbits and pigs. The prolonged action of B30-substituted insulins was markedly enhanced by B27 lysine or arginine substitutions and by B13 glutamine. The B27 residue is located on the surface of the hexamer, so a basic residue in this position presumably promotes the packing of hexamers at neutral pH. The B13 residues cluster in the centre of the hexamer. When the electrostatic repulsive forces from six glutamic acid residues are abolished by substitution with glutamine, a stabilization of the hexamer can be envisaged.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
410.
The dose of intravenous diazepam required for sedation was estimated in a series of 78 patients aged 17-85 years given the drug for dental and endoscopic procedures. Multiple regression analysis showed a significant correlation (r = 0.71; p less than 0.001) between dose and age, body weight, the taking of regular sedation, and the taking of more than 40 g alcohol daily, but there were no differences in the doses required between men and women, smokers and non-smokers, inpatients and outpatients, or dental and endoscopy patients. Patients aged 80 required an average dose of 10 mg and patients aged 20 an average dose of 30 mg, and the dose required was much higher in those receiving regular sedation or having a high alcohol intake. Plasma total and free diazepam concentrations were measured in the second half of the series of patients (n = 37). Plasma concentrations required for sedation fell twofold to threefold between the ages of 20 and 80 and were significantly higher in those taking regular sedation or alcohol. Differences in the acute response to diazepam appeared to be due to differences in the sensitivity of the central nervous system (pharmacodynamic tolerance) rather than to differences in pharmacokinetic factors. 相似文献