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91.
Transcription and Replication of Viral Deoxyribonucleic acid in Cells Coinfected with Adenovirus Types 2 and 12 总被引:8,自引:8,他引:0
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Stanley Mak 《Journal of virology》1969,4(5):651-656
The yield of infectious virus was determined for KB cells infected with both adenovirus types 2 (ad 2) and 12 (ad 12). It was found that the yield of the former was greatly reduced, whereas that of the latter was not affected significantly. The reduction in virus yield was accompanied by an inhibition of ad 2 virus-specific ribonucleic acid (RNA) and viral deoxyribonucleic acid (DNA) synthesis at various times after infection. On the other hand, the rate of synthesis of ad 12 virus-specific RNA and viral DNA was not inhibited, but advanced in time. The total amount of ad 12 viral DNA synthesized was not affected by coinfection with ad 2. These results suggest that ad 2 infection hastens the maturation of ad 12. 相似文献
92.
Summary Variations in extractable cellulase and pectinase were followed during development of Hemerocallis (day lily) flowers. A peak in cellulase activity occurs in the pistil just prior to anthesis, followed by a 62% diminution in the enzyme activity at the time of anthesis. Cellulase activity, per mg protein, is about twice as high in the upper (stigma) portion as in the middle and lower one-third of the pistil tissues. No pectinase activity was detected in the pistil at all stages of development. Extractable pectinase is present at a maximum level in the very young ovary; it decreases rapidly as the ovary develops. Cellulase remains at a moderate level of activity throughout the development of the ovary, except for an increase of about 50% at pollination. Soluble cellulase and pectinase are found in mature pollen. The changes in the cell-wall hydrolytic enzymes in the pistil were pollen-tube growth. It may also promote changes in the cell walls of the pistil cells, although metabolism of the middle lamella during pollen germination is primarily controlled by pollen pectinases.A contribution of the Florida Agricultural Experiment Station, Journal Series No. 3070. 相似文献
93.
Purification, Ultrastructure, and Composition of Axial Filaments from Leptospira 总被引:22,自引:16,他引:6
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The ultrastructure of three strains of water Leptospira was studied by negative staining, thin sectioning, and freeze-etching. The cells possessed a triple-layered sheath which covered two independent axial filaments, one inserted subterminally in each end of the cell. The protoplasmic cylinder was surrounded by a triple-layered cell wall and possessed ribosomes, lamellar structures, and a typical procaryotic nuclear region. The axial filament was comprised of several component structures. An axial fibril, with a diameter of 20 to 25 nm, consisted of a solid inner core (13 to 16 nm in diameter) surrounded by a coat. A terminal knob (40 to 70 nm in length) was connected to a series of disc insertion structures at the terminal end of the axial fibril. The axial fibril was surrounded by a helical outer coat (35 to 60 nm in diameter) which was composed of a continuously coiled fiber, 3 to 4 nm in diameter, embedded in an electron-dense material. A procedure for the purification of the axial fibrils was presented and their ultrastructural, physical, and chemical properties were determined. Similarities in ultrastructural, physical, and chemical properties were noted between the axial fibrils and bacterial flagella. A schematic model of the leptospiral axial filament is presented, and a mechanism is proposed for its function as a locomotor organelle. 相似文献
94.
95.
The relationship between the source strength and the “manifest vector” in the Einthoven Triangle is derived for a line and
a point dipole source and confirmed experimentally. The result permits the interpretation of the standard ECG leads in absolute
terms and corrected for body size. The manifest vector is shown to be approximately
times what it would be in an otherwise similar circular slab which circumscribes the triangle. 相似文献
96.
Morphology and Reproductive Processes of the L Forms of Bacteria II. Comparative Study of L Forms and Mycoplasma with the Electron Microscope 总被引:3,自引:0,他引:3
Representative electron micrographs, from the study of eight strains of L forms and one strain of Mycoplasma, are presented. A- and B-type L forms were derived from two strains of Proteus, two other L forms were derived from a diphtheroid and from a staphylococcus strain, and two strains (designated as LX) were isolated from L forms derived from a group A beta-hemolytic streptococcus and from a staphylococcus. The Mycoplasma strain was isolated from goats. Sections were made of young colonies grown within agar and from parts of surface colonies embedded in the agar. B-type L colonies of Proteus were produced by inoculation of bacteria into media containing penicillin. The large bodies developing from the bacteria and the organisms in B-type L colonies of Proteus, like the parent bacteria, had a cell wall consisting of a plasma membrane and an outer cell wall. The loss of rigidity in the cell wall indicated an alteration in its structure. The A-type L cultures of Proteus consisted of irregular branching masses extending in several directions, of small dense organisms corresponding to the elementary corpuscles present in cultures of Mycoplasma, and of intermediary forms. In contrast to the B-type, all organisms in the A-type colonies were surrounded by a single unit membrane corresponding to the plasma membrane of bacteria. The structures inside the cell membrane, both in the A- and B-type, seemed to correspond to the structure of the parent bacteria, which contained ribosomes and threads of DNA. The elementary corpuscles formed chains and filaments, and, apparently, these corpuscles took part in the multiplication by gradual enlargement. The organisms seen in the cultures of all L forms and Mycoplasma studied, except in the B-type L forms of Proteus, corresponded in size, shape, and structure, as well as in the development of elementary corpuscles, to the organisms in the A-type L form of Proteus. In contrast to the spherical organisms usually seen in broth cultures, the organisms in young cultures of Mycoplasma, which were grown within the agar, were similar in morphology, as well as in the discernible structure of the organisms, to L forms. Significant morphological and structural differences were not apparent between the L forms and Mycoplasma (in cultures grown within agar media) under the conditions of this investigation. 相似文献
97.
Cation Transport in Escherichia coli : VII. Potassium requirement for phosphate uptake 总被引:13,自引:1,他引:12
Paul L. Weiden Wolfgang Epstein Stanley G. Schultz 《The Journal of general physiology》1967,50(6):1641-1661
When Escherichia coli K-12 is grown in media containing limiting amounts of K, growth continues normally until all the extracellular K has been consumed. Thereafter the rates of growth, glucose consumption, and oxygen consumption decrease progressively, and the cell contents of K and P fall. These changes, referred to as K limitation, are all reversed by the addition of K. By specifically altering the ionic composition of the cells it was shown that these metabolic disturbances are not due to changes in the cell content of K or Na, but are directly related to the absence of K from the extracellular medium. The cell pool of inorganic P and the uptake of PO4 from the medium are low in K-limited cells and are immediately stimulated by the addition of K, suggesting that the primary effect of K limitation is to inhibit PO4 uptake. All the metabolic effects of K limitation can be attributed to inhibition of PO4 uptake. The requirement of extracellular K for PO4 uptake may be due to a coupling between the uptake of K and PO4. 相似文献
98.
99.
Stanley G. Schultz Ralph Zalusky Arthur E. Gass Jr. 《The Journal of general physiology》1964,48(2):375-378
Unidirectional Cl fluxes across in vitro segments of rabbit ileum have been determined both in the absence and in the presence of an electrochemical potential gradient. The results indicate that Cl transport in this preparation can be attributed to purely passive forces uninfluenced by solvent drag or exchange diffusion. Furthermore, on the basis of this and previous studies, it has been demonstrated that the sum of the partial ionic conductances of Na and Cl accounts for at least 90 per cent of the total tissue conductance. 相似文献
100.
The European corn borer, Ostrinia nubilalis (Hübn.), has been reared satisfactorily on a meridic diet containing no crude plant material. Ascorbic acid has been shown to be a necessary component of this diet for normal development of the larvae. Evidence is presented of the identity of the corn leaf factor (CLF), contained in spray-dried alfalfa juice, with ascorbic acid. The larvae grew better on a wheat germ supplemented diet than on the diets in which wheat germ was replaced with powdered cellulose, vitamin-free casein and corn oil or wheat germ oil. These data suggested that another factor, present in wheat germ, was required for optimal larval growth.
Approved for publication by the director of the Wisconsin Agricultural Experiment Station.
This study was supported in part by research grant (GM-05093) from the National Institutes of Health. 相似文献
Zusammenfassung Der Maiszünsler, Ostrinia nubilalis (Hübn.), wurde zufriedenstellend mit einer meridischen (meridic) Diät aufgezogen, die kein pflanzliches Rohmaterial enthielt. Es zeigte sich, daß zur normalen Entwicklung der Raupen zu dieser Diät Ascorbinsäure zugesetzt werden muß. Es wird wahrscheinlich gemacht, daß der Mais-Blattfaktor (CLF), der in sprühgetrocknetem Luzernesaft enthalten ist, mit Ascorbinsäure identisch ist.Die Larven wuchsen an der ergänzten Weizenkeimlingsdiät besser als an Diäten, bei denen die Weizenkeimlinge durch staubförmige Cellulose, vitaminfreies Kasein und Maisöl oder Weizenkeimlingsöl ersetzt war. Diese Befunde lassen vermuten, daß zum optimalen Wachstum ein anderer, in Weizenkeimlingen enthaltener Faktor erforderlich ist.Die Entdeckung der Ascorbinsäure als Mais-Blattfaktor ermöglicht die Entwicklung einer holidischen (holidic) Diät für dieses Insekt. Solch eine holidische Diät wird jedoch solange unteroptimal sein, bis der Faktor in Weizenkeim bestimmt ist.
Approved for publication by the director of the Wisconsin Agricultural Experiment Station.
This study was supported in part by research grant (GM-05093) from the National Institutes of Health. 相似文献