Model Building and Analysis for Block Designs with Nested Rows and Columns

The paper deals with two main problems: it gives a unified approach to model building for observations obtained in a block design with nested rows and columns and it provides the analysis of variance for such data. Also, some statistical properties of the design are examined.     

Tide-dominated Middle Devonian sequence from the northern part of the Holy Cross Mountains (Central Poland)

Summary The dolomitic Wojciechowice Formation distinctly differs from the remaining, mainly shaly Middle Devonian succession in northern part of the Holy Cross Mountains (Central Poland). The upper Member of the Formation (Crystalline Dolostone Mb.), in greater part dolomitized but also containing limestone beds, is composed of shallowing-upward cyclothems well exposed in Skaly quarry in the Bodzentyn syncline. The lower parts of the cyclothems, interpreted as subtidal facies, contain fossils characteristic for restricted environments. They are grouped into two assemblages. The first, with brachiopods (largeBornhardtina andEmanuella), massive stromatoporoids, and subordinate gastropods and amphiporoids is related to a deeper subtidal environment, while the second (mainly amphiporoids, gastropods, ostracodes and calcareous algae) is shallower subtidal. Towards the top of succession the fossil content radically decreases. The upper parts of cyclothems are composed mainly of different types of laminites. In these parts of the section, interpreted as intertidal/supratidal units, stromatolites, desiccation polygons, intraformational breccias, and common bioturbations are present. The whole succession was deposited in a low-energy environment, only intermittently affected by high-energy events. For their most spectacular example of this, aBornhardtina-coquinite, a tempestitic origin is proposed. The interval with cyclic sedimentation studied correlates with the dolomitized lower “Unit I” of the Stromatoporoid-Coral Kowala Formation from the southern part of the Holy Cross Mountains, which exhibits sabkha-type cyclicity. The differences in development of cyclothems in both regions resemble outer and inner part of an extensive platform, and correspond well with basic trends of the Lower-Middle Devonian transgression in the Holy Cross Mountains. The general succession of formations deposited during this process coincides with transgressive events on Johnson's eustatic curve for the Devonian.     

Absence of 6-Hydroxydopamine in the Rat Brain After Treatment with Stimulants and Other Dopaminergic Agents: A Mass Fragmentographic Study

Abstract: Formation of 6-hydroxydopamine (6-OHDA) from dopamine has been hypothesized to mediate neuro-degeneration induced by some psychostimulants. Although the emergence of a 6-OHDA-like substance was reported in the striatum of methamphetamine-treated rats, this substance has not been identified by a direct approach. We used mass fragmentography to search for 6-OHDA in the rat frontal cortex and striatum after the administration of a number of drugs including 3,4-dihy-droxyphenyl-L-alanine, methamphetamine, amphetamine, and cocaine, all of which increase synaptic dopamine. No 6-OHDA was detected after the acute systemic administration of these agents. Intraventricular administration of 6-OHDA (10 μg/rat.) produced measurable concentrations of 6-OHDA that were higher in the striatum than in the frontal cortex. Intraventricular administration of 2,4,5-trihydroxy-phenyl-D,L-alanine (6-OHDOPA; 10 μg/rat) produced similar concentrations of 6-OHDA in both regions. Pargyline, but not carbidopa (α-methyldopahydrazine), enhanced the effect of intraperitoneal 6-OHDOPA administration (80 mg/kg). We conclude that (1) 6-OHDOPA can cross the blood-brain barrier and is converted to 6-OHDA in the brain, (2) 6-OHDA is a substrate for monoamine oxidase(s) and therefore a search for its purported deaminated metabolite is warranted, and (3) acute treatment with the above stimulants either does not lead to the formation of 6-OHDA or produces concentrations below the detection limit of the assay (<34 pg/mg of protein).     

Role of leptin in the stomach and the pancreas

Leptin, a 16 kDa protein encoded by the ob gene, is known mainly for its role in the regulation of food intake, body composition and energy expenditure through a central feedback mechanism. Initially leptin was considered as an ob gene product of adipocytes but recently the presence of leptin and its receptors have been revealed in other organs including gastric mucosa and the pancreas and found to be released from these organs by cholecystokinin (CCK), gastrin and ordinary feeding. Furthermore, leptin was found to mimic the action of CCK on gastric and pancreatic integrity, while reducing the food intake and to affect gastric and pancreatic secretion. This report emphasizes the role of leptin originating from the gastrointestinal tract acting synergistically with CCK at the hypothalamus level on the mechanism of food intake and locally on the protection of gastric mucosa and the pancreas against noxious agents and to maintain tissue integrity.