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271.
Direct reaction of NAD(P)H with oxidants like singlet oxygen ((1)O(2)) has not yet been demonstrated in biological systems. We therefore chose different rhodamine derivatives (tetramethylrhodamine methyl ester, TMRM; 2',4',5',7'-tetrabromorhodamine 123 bromide; and rhodamine 123; Rho 123) to selectively generate singlet oxygen within the NAD(P)H-rich mitochondrial matrix of cultured hepatocytes. In a cell-free system, photoactivation of all of these dyes led to the formation of (1)O(2), which readily oxidized NAD(P)H to NAD(P)(+). In hepatocytes loaded with the various dyes only TMRM and Rho 123 proved suited to generating (1)O(2) within the mitochondrial matrix space. Photoactivation of the intracellular dyes (TMRM for 5-10 s, Rho 123 for 60 s) led to a significant (29.6 +/- 8.2 and 30.2 +/- 5.2%) and rapid decrease in mitochondrial NAD(P)H fluorescence followed by a slow increase. Prolonged photoactivation (> or =15 s) of TMRM-loaded cells resulted in even stronger NAD(P)H oxidation, the rapid onset of mitochondrial permeability transition, and apoptotic cell death. These results demonstrate that NAD(P)H is the primary target for (1)O(2) in hepatocyte mitochondria. Thus NAD(P)H may operate directly as an intracellular antioxidant, as long as it is regenerated. At cell-injurious concentrations of the oxidant, however, NAD(P)H depletion may be the event that triggers cell death. 相似文献
272.
This paper is concerned with large scale associative memory design. A serious problem with neural associative memories is the quadratic growth of the number of interconnections with the problem size. An overlapping decomposition algorithm is proposed to attack this problem. Specifically, a pattern to be processed is decomposed into overlapping sub-patterns. Then, neural sub-networks are constructed that process the sub-patterns. An error correction algorithm operates on the outputs of each sub-network in order to correct the mismatches between sub-patterns that are obtained from the independent recall processes of individual sub-networks. The performance of the proposed large scale associative memory is illustrated using two-dimensional images. It is shown that the proposed method reduces the computing cost of the design of the associative memories compared with non-interconnected associative memories. 相似文献
273.
The enzyme indole-3-acetylglucose synthase (UDPG: indole-3-ylacetylglucosyl transferase) catalyzes the reaction: UDPG + IAA 1-O-IAGlc + UDP. The enzyme is abundantly present inimmature maize endosperm, but present in lesser amount in the endosperm ofgerminating kernels. Rabbit polyclonal antibodies, against purified IAGlcsynthase, easily visualize the presence of the enzyme protein in endosperm, butnot in vegetative tissue. However, after 4 to 8 h of incubation ofmesocotyl and coleoptile segments in 50 M 1-naphthalene aceticacid (NAA) solution, the IAGlc synthase protein is detectable by Western blotanalysis, and enzyme activity determined in whole tissue homogenate is alsoincreased. Induction of IAGlc synthase by NAA is inhibited by cycloheximide. 相似文献
274.
Boguszewska A Tchórzewski M Dukowski P Winiarczyk S Grankowski N 《Biology of the cell / under the auspices of the European Cell Biology Organization》2002,94(3):139-146
The yeast ribosomal "stalk"--a lateral protuberance on the 60S subunit--consists of four acidic P-proteins, P1A, P1B, P2A and P2B, which play an important role during protein synthesis. Contrary to most ribosomal proteins, which are rapidly degraded in the cytoplasm, P-proteins are found as a cytoplasmic pool and are exchanged with the ribosome-bound proteins during translation. As yet, subcellular trafficking of P-proteins has not been extensively investigated. Therefore, we have characterized--using immunological approaches--the cellular distribution of P-proteins in several environmental conditions, characteristic of yeast cells, such as growth phases, and heat-, osmotic-, and oxygen-stress. Using the western blotting approach, we have shown P-proteins to be present in constant amounts on the ribosomes, despite their exchangeability with the cytoplasmic pool, and regardless of environmental conditions. On the other hand, P-protein level in the cytoplasm decreased sharply throughout the consecutive growth phases, but was not affected by several stress conditions. Applying the electron microscopic technique and immunogold labeling, we have found that P-proteins are located in two cell compartments. The first one is the cytoplasm and the second one--an unexpected place--the cell wall, where P-proteins are fully phosphorylated. Moreover, the existence of P-proteins on the cellular wall is not affected by various environmental conditions. 相似文献
275.
A prospective randomized trial was used to determine iron concentrations in intestinal cancer tissue and colorectum polyps.
We investigated the possible difference between the concentrations of iron, ferritin, albumin, and hemoglobin in the serum
of patients with colorectal cancer and polyps. We also determined the relationship between the iron and ferritin levels in
cancer tissue, the localization of neoplasms, and the stage of their development. The study comprises 67 patients with colorectum
cancer and 42 patients with colon and rectum polyps. The metal was determined by using the total-reflection X-ray fluorescence
(TRXRF) method. The mean concentration of iron in colorectal cancer equaled 46.1 μg/g of the tissue and was higher than in
the case of polyps (43.2 μg/g). The mean serum iron level in patients with colorectal cancer was statistically lower than
in the serum of patients with polyp and in the control group (54.5, 91.3, and 108.0 μg/g, respectively). The determined average
concentration of ferritin in the serum of patients with colorectal cancer equaled 60.4 μg/g and was statistically lower than
the level of this enzyme in the serum of patients with polyps (85.2 μg/g) and in the control group (102.0 μg/g). There was
no difference between the serum albumin and hemoglobin concentrations in patients with colorectal cancer, polyps, and the
control. There was no difference in the levels of iron and ferritin depending on the location of the neoplasm and the stage
of its development. Also, there was no difference between the concentrations of iron in the cancer tissue of malignant and
benign tumors after taking into consideration sex and age of patients. During the examination we determined significantly
higher concentrations of iron in the cancer tissue and not in the polyp. The low levels of iron in the serum of patients with
malignant tumor may increase colorectal cancer risk. 相似文献
276.
Fryer MJ Ball L Oxborough K Karpinski S Mullineaux PM Baker NR 《The Plant journal : for cell and molecular biology》2003,33(4):691-705
In Arabidopsis leaves, high light stress induces rapid expression of a gene encoding a cytosolic ascorbate peroxidase (APX2), whose expression is restricted to bundle sheath cells of the vascular tissue. Imaging of chlorophyll fluorescence and the production of reactive oxygen species (ROS) indicated that APX2 expression followed a localised increase in hydrogen peroxide (H2O2) resulting from photosynthetic electron transport in the bundle sheath cells. Furthermore, leaf transpiration rate also increased prior to APX2 expression, suggesting that water status may also be involved in the signalling pathway. Abscisic acid stimulated APX2 expression. Exposure of ABA-insensitive mutants (abi1-1, abi2-1) to excess light resulted in reduced levels of APX2 expression and confirmed a role for ABA in the signalling pathway. ABA appears to augment the role of H2O2 in initiating APX2 expression. This regulation of APX2 may reflect a functional organisation of the leaf to resolve two conflicting physiological requirements of protecting the sites of primary photosynthesis from ROS and, at the same time, stimulating ROS accumulation to signal responses to changes in the light environment. 相似文献
277.
278.
Fox K Wallace H Glazewski S 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2002,357(1428):1709-1715
Sensory deprivation and injury to the peripheral nervous system both induce plasticity in the somatosensory system of adult animals, but in different places. While injury induces plasticity at several locations within the ascending somatosensory pathways, sensory deprivation appears only to affect the somatosensory cortex. Experiments have been performed to detect experience-dependent plasticity in thalamic receptive fields, thalamic domain sizes and convergence of thalamic receptive fields onto cortical cells. So far, plasticity has not been detected with sensory deprivation paradigms that cause substantial cortical plasticity. Part of the reason for the lack of thalamic plasticity may lie in the synaptic properties of afferent systems to the thalamus. A second factor may lie in the differences in the organization of cortical and thalamic circuits. Many deprivation paradigms induce plasticity by decreasing phasic lateral inhibition. Since lateral inhibition appears to be far weaker in the thalamus than the cortex, sensory deprivation may not cause large enough imbalances in thalamic activity to induce plasticity in the thalamus. 相似文献
279.
UDP-glucose pyrophosphorylase (UGPase) is an important enzyme of synthesis of sucrose, cellulose, and several other polysaccharides in all plants. The protein is evolutionarily conserved among eukaryotes, but has little relation, aside from its catalytic reaction, to UGPases of prokaryotic origin. Using protein homology modeling strategy, 3D structures for barley, poplar, and Arabidopsis UGPases have been derived, based on recently published crystal structure of human UDP-N-acetylglucosamine pyrophosphorylase. The derived 3D structures correspond to a bowl-shaped protein with the active site at a central groove, and a C-terminal domain that includes a loop (I-loop) possibly involved in dimerization. Data on a plethora of earlier described UGPase mutants from a variety of eukaryotic organisms have been revisited, and we have, in most cases, verified the role of each mutation in enzyme catalysis/regulation/structural integrity. We have also found that one of two alternatively spliced forms of poplar UGPase has a very short I-loop, suggesting differences in oligomerization ability of the two isozymes. The derivation of the structural model for plant UGPase should serve as a useful blueprint for further function/structure studies on this protein. 相似文献
280.