全文获取类型
收费全文 | 1094篇 |
免费 | 74篇 |
国内免费 | 3篇 |
专业分类
1171篇 |
出版年
2023年 | 3篇 |
2022年 | 12篇 |
2021年 | 17篇 |
2020年 | 14篇 |
2019年 | 23篇 |
2018年 | 26篇 |
2017年 | 16篇 |
2016年 | 37篇 |
2015年 | 42篇 |
2014年 | 49篇 |
2013年 | 64篇 |
2012年 | 94篇 |
2011年 | 102篇 |
2010年 | 67篇 |
2009年 | 52篇 |
2008年 | 68篇 |
2007年 | 80篇 |
2006年 | 54篇 |
2005年 | 60篇 |
2004年 | 59篇 |
2003年 | 42篇 |
2002年 | 45篇 |
2001年 | 16篇 |
2000年 | 15篇 |
1999年 | 10篇 |
1998年 | 12篇 |
1997年 | 7篇 |
1996年 | 12篇 |
1995年 | 14篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1989年 | 2篇 |
1987年 | 2篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1982年 | 10篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1974年 | 4篇 |
1967年 | 1篇 |
1965年 | 1篇 |
1960年 | 1篇 |
1958年 | 1篇 |
1956年 | 1篇 |
1935年 | 1篇 |
1925年 | 1篇 |
排序方式: 共有1171条查询结果,搜索用时 15 毫秒
21.
Francová Kateřina Šumberová Kateřina Kučerová Andrea Šorf Michal Grill Stanislav Exler Norbert Vrba Jaroslav 《Wetlands Ecology and Management》2021,29(1):93-110
Wetlands Ecology and Management - Plants play an important role in fishpond littorals, but little is known about factors influencing their presence and growth patterns. We surveyed vegetation of... 相似文献
22.
Stanislav Kadl?ík Tomá? Ku?era Dominika Chalupská Radek Ga?ák Markéta Koběrská Dana Ulanová Jan Kopecky Eva Kutejová Lucie Najmanová Ji?í Janata 《PloS one》2013,8(12)
Clinically used lincosamide antibiotic lincomycin incorporates in its structure 4-propyl-L-proline (PPL), an unusual amino acid, while celesticetin, a less efficient related compound, makes use of proteinogenic L-proline. Biochemical characterization, as well as phylogenetic analysis and homology modelling combined with the molecular dynamics simulation were employed for complex comparative analysis of the orthologous protein pair LmbC and CcbC from the biosynthesis of lincomycin and celesticetin, respectively. The analysis proved the compared proteins to be the stand-alone adenylation domains strictly preferring their own natural substrate, PPL or L-proline. The LmbC substrate binding pocket is adapted to accomodate a rare PPL precursor. When compared with L-proline specific ones, several large amino acid residues were replaced by smaller ones opening a channel which allowed the alkyl side chain of PPL to be accommodated. One of the most important differences, that of the residue corresponding to V306 in CcbC changing to G308 in LmbC, was investigated in vitro and in silico. Moreover, the substrate binding pocket rearrangement also allowed LmbC to effectively adenylate 4-butyl-L-proline and 4-pentyl-L-proline, substrates with even longer alkyl side chains, producing more potent lincosamides. A shift of LmbC substrate specificity appears to be an integral part of biosynthetic pathway adaptation to the PPL acquisition. A set of genes presumably coding for the PPL biosynthesis is present in the lincomycin - but not in the celesticetin cluster; their homologs are found in biosynthetic clusters of some pyrrolobenzodiazepines (PBD) and hormaomycin. Whereas in the PBD and hormaomycin pathways the arising precursors are condensed to another amino acid moiety, the LmbC protein is the first functionally proved part of a unique condensation enzyme connecting PPL to the specialized amino sugar building unit. 相似文献
23.
Elena V. Gorb Alexander E. Filippov Stanislav N. Gorb 《Arthropod-Plant Interactions》2013,7(3):277-286
Myrmecochory or seed dispersal by ants is a widely spread phenomenon, and myrmecochorous plants constitute a large portion of species in many ecosystems. Since the ant species complex in the ecosystem is continuously changing in time and space, the long-term effects of such ant–plant interactions on the plant community remained unclear. The manifold information obtained in numerous previous studies from one ecosystem in the deciduous forests of Central Ukraine allowed us to simulate the possible scenarios for plant survival and distribution in the ecosystem after a reduction in the number or local extinction of one of the ant species. The results of the virtual long-term experiment show that the abundance and spatial distribution of myrmecochorous plants strongly depends on both the abundance of ants and their species composition in the ecosystem. The positive role of ant species diversity for maintaining myrmecochorous plant species diversity is shown. Competition between plant species for seed dispersers is influenced by the ant community in such a way that the disappearance of one ant species may lead to the reduction or even local extinction of a particular plant population. 相似文献
24.
Ivana Shawkatová Juraj Javor Zuzana Párnická Peter Kozub Mária Žilínková Peter Frey Stanislav Ferenčík Milan Buc 《Folia microbiologica》2013,58(4):319-324
Psoriasis vulgaris is a complex chronic skin disease with immunological and genetic background. The most important predisposing genetic factors in psoriasis are genes of the human leukocyte antigen (HLA) region. Accumulative evidence has shown that several HLA alleles are closely associated with psoriasis; however, they tend to vary in different racial and ethnic backgrounds. One hundred forty-seven unrelated Slovak patients with psoriasis vulgaris (average age at onset 28?±?14 years) were genotyped for the HLA-C, DQB1 and DRB1 alleles by the polymerase chain reaction using sequence-specific primers. Allele frequencies observed in the group of psoriatic patients were compared to those obtained in the ethnically matched control group comprising 194 subjects with no history of psoriasis. Susceptibility to psoriasis vulgaris in our study group is significantly associated with HLA-C*06 (odds ratio (OR)?=?3.85), DRB1*07 (OR?=?2.56) and DQB1*02 (OR?=?1.09), respectively, whereas DRB*01 (OR?=?0.05) is associated negatively. Hereby, we provide the first report on the association of HLA-C, DRB1 and DQB1 alleles with psoriasis in the Slovak population. Our findings confirm HLA-C*06 and DRB1*07 as the most important genetic risk factors for psoriasis. However, the role of HLA genes as causative in the pathogenesis of the disease remains unclear. Identification of genetic factors that increase the risk of psoriasis is a precondition that helps to elucidate the pathogenesis of this troubling disease and identify targets for a more specific and effective therapy. 相似文献
25.
Inter‐Fullerene Electronic Coupling Controls the Efficiency of Photoinduced Charge Generation in Organic Bulk Heterojunctions 下载免费PDF全文
Bryon W. Larson Obadiah G. Reid David C. Coffey Stanislav M. Avdoshenko Alexey A. Popov Olga V. Boltalina Steven H. Strauss Nikos Kopidakis Garry Rumbles 《Liver Transplantation》2016,6(24)
Photoinduced charge generation (PCG) dynamics are notoriously difficult to correlate with specific molecular properties in device relevant polymer:fullerene organic photovoltaic blend films due to the highly complex nature of the solid state blend morphology. Here, this study uses six judiciously selected trifluoromethylfullerenes blended with the prototypical polymer poly(3‐hexylthiophene) and measure the PCG dynamics in 50 fs–500 ns time scales with time‐resolved microwave conductivity and femtosecond transient absorption spectroscopy. The isomeric purity and thorough chemical characterization of the fullerenes used in this study allow for a detailed correlation between molecular properties, driving force, local intermolecular electronic coupling and, ultimately, the efficiency of PCG yield. The findings show that the molecular design of the fullerene not only determines inter‐fullerene electronic coupling, but also influences the decay dynamics of free holes in the donor phase even when the polymer microstructure remains unchanged. 相似文献
26.
Michal Malček Lukáš Bučinský Zuzana Barbieriková Sandra Dorotíková Dana Dvoranová Vlasta Brezová Peter Rapta Stanislav Biskupič 《Journal of molecular modeling》2016,22(10):251
Protonation in the two-electron/two-proton reduction processes of 2,6-dichlorophenolindophenolate (DCIP) is investigated combining density functional theory (DFT) and molecular dynamics (MD) methods. DCIP (anion), DCIP?– (radical anion), and DCIP2? (dianion) are considered, including the electronic structure analysis from the prospective of quantum theory of atoms and molecules (QTAIM). It is shown that oxygen on the indophenolate moiety and nitrogen are the first and/or the second proton acceptor sites and their energetic order depends on the total charge of the system. MD simulations of differently charged species interacting with the solvent molecules have been performed for methanol, water, and oxonium cation (H3O+). Methanol and water molecules are found to form only hydrogen bonds with the solute irrespective of its charge. The calculated pKa values show that the imino group of DCIPH? is a weaker acid than water. While in the case of DCIP (and DCIP?–) plus oxonium cation, proton transfer from the solvent to the solute was evidenced for both aforementioned acceptor sites. In addition, MD simulations of bulks containing 15 and 43 molecules of water around the DCIP molecule have been performed, revealing the formation of 2–4 hydrogen bonds. 相似文献
27.
Benjamin Lamp Angelika Url Kerstin Seitz Jürgen Eichhorn Christiane Riedel Leonie Janina Sinn Stanislav Indik Hemma K?glberger Till Rümenapf 《PloS one》2016,11(11)
European honey bees are highly important in crop pollination, increasing the value of global agricultural production by billions of dollars. Current knowledge about virulence and pathogenicity of Deformed wing virus (DWV), a major factor in honey bee colony mortality, is limited. With this study, we close the gap between field research and laboratory investigations by establishing a complete in vitro model for DWV pathogenesis. Infectious DWV was rescued from a molecular clone of a DWV-A genome that induces DWV symptoms such as crippled wings and discoloration. The expression of DWV proteins, production of infectious virus progeny, and DWV host cell tropism could be confirmed using newly generated anti-DWV monoclonal antibodies. The recombinant RNA fulfills Koch’s postulates circumventing the need of virus isolation and propagation of pure virus cultures. In conclusion, we describe the development and application of a reverse genetics system for the study of DWV pathogenesis. 相似文献
28.
Stanislav Sobolevsky Izabela Sitko Remi Tachet des Combes Bartosz Hawelka Juan Murillo Arias Carlo Ratti 《PloS one》2016,11(2)
Scientific studies of society increasingly rely on digital traces produced by various aspects of human activity. In this paper, we exploit a relatively unexplored source of data–anonymized records of bank card transactions collected in Spain by a big European bank, and propose a new classification scheme of cities based on the economic behavior of their residents. First, we study how individual spending behavior is qualitatively and quantitatively affected by various factors such as customer’s age, gender, and size of his/her home city. We show that, similar to other socioeconomic urban quantities, individual spending activity exhibits a statistically significant superlinear scaling with city size. With respect to the general trends, we quantify the distinctive signature of each city in terms of residents’ spending behavior, independently from the effects of scale and demographic heterogeneity. Based on the comparison of city signatures, we build a novel classification of cities across Spain in three categories. That classification exhibits a substantial stability over different city definitions and connects with a meaningful socioeconomic interpretation. Furthermore, it corresponds with the ability of cities to attract foreign visitors, which is a particularly remarkable finding given that the classification was based exclusively on the behavioral patterns of city residents. This highlights the far-reaching applicability of the presented classification approach and its ability to discover patterns that go beyond the quantities directly involved in it. 相似文献
29.