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61.
Rats were trained to discriminate short or long durations of houselight illumination using a choice procedure. During the test phase of each trial, the left and right levers were presented with an auditory cue above one of them on (cued lever) while the other was off (uncued lever). The auditory cue was presented immediately after sample offset and the levers were inserted after the auditory cue had been presented for 2 s. For half of the rats, the correct response following the short sample was to press the cued lever, while following the long sample, it was to press the uncued lever. This was reversed for the remaining rats. Following acquisition of the discrimination, two different types of delay tests were administered. In the first set, the delay between offset of the sample and onset of the auditory cue was manipulated (Cue Delay Test). In the second set, the delay between onset of the auditory cue and entry of the levers into the chamber was manipulated (Response Delay Test). Cue Delay testing resulted in a choose-long bias at the longer delays. Response Delay testing did not result in a systematic response bias and there was little forgetting over the delay interval. These data suggest that the rats did not stop the internal clock when the nominal sample was offset, but allowed it to keep running until the auditory cue was presented. The data from the Response Delay Test indicate that either a response decision was made based on the clock reading as soon as the auditory cue was presented, or the clock reading itself was retained over the delay with no subjective shortening and little forgetting. 相似文献
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Harry Balch Stanford Splitter Paul Flynn Laurance W. Kinsell 《The Western journal of medicine》1958,89(3):165-168
Atherosclerosis is the Number One public health problem. Many factors have been implicated in the pathogenesis of this disease. Prominent among these factors is the amount and kind of fat in the diet. The evidence now appears to be conclusive that sufficient quantities of polyunsaturated fat in the diet, with proportional decrease in saturated fat, will result in major decrease in blood lipid. Some evidence indicates that such blood lipid lowering produces a desirable effect upon existing atherosclerosis. Much additional time and work will be required to clarify the prophylactic and therapeutic value of this type of dietary approach. 相似文献
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Heat and desiccation are the predominant factors affecting inactivation of Bacillus licheniformis and Bacillus thuringiensis spores during simulated composting
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67.
Standardized experiments in mutant mice reveal behavioural similarity on 129S5 and C57BL/6J backgrounds
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L. N. van de Lagemaat L. E. Stanford C. M. Pettit D. J. Strathdee K. E. Strathdee K. A. Elsegood D. G. Fricker M. D. R. Croning N. H. Komiyama S. G. N. Grant 《Genes, Brain & Behavior》2017,16(4):409-418
Behavioural analysis of mice carrying engineered mutations is widely used to identify roles of specific genes in components of the mammalian behavioural repertoire. The reproducibility and robustness of phenotypic measures has become a concern that undermines the use of mouse genetic models for translational studies. Contributing factors include low individual study power, non‐standardized behavioural testing, failure to address confounds and differences in genetic background of mutant mice. We have examined the importance of these factors using a statistically robust approach applied to behavioural data obtained from three mouse mutations on 129S5 and C57BL/6J backgrounds generated in a standardized battery of five behavioural assays. The largest confounding effect was sampling variation, which partially masked the genetic background effect. Our observations suggest that strong interaction of mutation with genetic background in mice in innate and learned behaviours is not necessarily to be expected. We found composite measures of innate and learned behaviour were similarly impacted by mutations across backgrounds. We determined that, for frequently used group sizes, a single retest of a significant result conforming to the commonly used P < 0.05 threshold results in a reproducibility of 60% between identical experiments. Reproducibility was reduced in the presence of strain differences. We also identified a P‐value threshold that maximized reproducibility of mutant phenotypes across strains. This study illustrates the value of standardized approaches for quantitative assessment of behavioural phenotypes and highlights approaches that may improve the translational value of mouse behavioural studies. 相似文献
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Identification of mutations from phenotype-driven ENU mutagenesis in mouse Chromosome 7 总被引:1,自引:1,他引:0
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D. S. Pavlov K. A. Savvaitova K. V. Kuzishchin M. A. Gruzdeva A. Yu. Mal’tsev J. A. Stanford 《Journal of Ichthyology》2008,48(1):37-44
The diversity of life strategies and population structure of Kamchatka mykiss Parasalmo mykiss in the ecosystems of small salmon rivers of various types are considered. Hydrogeomorphological differences of the model rivers Kol and Kekhta are found. The preferred habitats are investigated, and their area is determined. It is shown that, in the Kol River, the mykiss having a resident life strategy predominated, and in the Kekhta River—the mykiss with migratory strategy. The key parameter controlling the prevalence of life strategies in each river is the ratio of the area of spawning grounds to the area of feeding grounds and their productivity. The hypothesis is confirmed that, in complex river systems, due to the diversity of biotopes and a higher productivity, the food resources are sufficient for maturation of mykiss and for realization of the resident life strategy (without migration to the sea for feeding). In small rivers of the channel type with their insufficient food resources, the specimens having a migratory life strategy prevail. 相似文献