排序方式: 共有35条查询结果,搜索用时 0 毫秒
31.
Peifang Sun Josefina García Guillermo Comach Maryanne T. Vahey Zhining Wang Brett M. Forshey Amy C. Morrison Gloria Sierra Isabel Bazan Claudio Rocha Stalin Vilcarromero Patrick J. Blair Thomas W. Scott Daria E. Camacho Christian F. Ockenhouse Eric S. Halsey Tadeusz J. Kochel 《PLoS neglected tropical diseases》2013,7(7)
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Lesley J. Drake Sarman Singh C. K. Mishra Amarjeet Sinha Sanjay Kumar Rajesh Bhushan T. Deirdre Hollingsworth Laura J. Appleby Rakesh Kumar Kriti Sharma Yogita Kumar Sri Raman Stalin Chakrabarty Jimmy H. Kihara N. K. Gunawardena Grace Hollister Vandana Kumar Anish Ankur Babul Prasad Sushma Ramachandran Alissa Fishbane Prerna Makkar 《PLoS neglected tropical diseases》2015,9(11)
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Costes B Fournier G Michel B Delforge C Raj VS Dewals B Gillet L Drion P Body A Schynts F Lieffrig F Vanderplasschen A 《Journal of virology》2008,82(10):4955-4964
Koi herpesvirus (KHV) is the causative agent of a lethal disease in koi and common carp. In the present study, we describe the cloning of the KHV genome as a stable and infectious bacterial artificial chromosome (BAC) clone that can be used to produce KHV recombinant strains. This goal was achieved by the insertion of a loxP-flanked BAC cassette into the thymidine kinase (TK) locus. This insertion led to a BAC plasmid that was stably maintained in bacteria and was able to regenerate virions when permissive cells were transfected with the plasmid. Reconstituted virions free of the BAC cassette but carrying a disrupted TK locus (the FL BAC-excised strain) were produced by the transfection of Cre recombinase-expressing cells with the BAC. Similarly, virions with a wild-type revertant TK sequence (the FL BAC revertant strain) were produced by the cotransfection of cells with the BAC and a DNA fragment encoding the wild-type TK sequence. Reconstituted recombinant viruses were compared to the wild-type parental virus in vitro and in vivo. The FL BAC revertant strain and the FL BAC-excised strain replicated comparably to the parental FL strain. The FL BAC revertant strain induced KHV infection in koi carp that was indistinguishable from that induced by the parental strain, while the FL BAC-excised strain exhibited a partially attenuated phenotype. Finally, the usefulness of the KHV BAC for recombination studies was demonstrated by the production of an ORF16-deleted strain by using prokaryotic recombination technology. The availability of the KHV BAC is an important advance that will allow the study of viral genes involved in KHV pathogenesis, as well as the production of attenuated recombinant candidate vaccines. 相似文献
34.
Bart L. Haagmans Judith M. A. van den Brand Lisette B. Provacia V. Stalin Raj Koert J. Stittelaar Sarah Getu Leon de Waal Theo M. Bestebroer Geert van Amerongen Georges M. G. M. Verjans Ron A. M. Fouchier Saskia L. Smits Thijs Kuiken Albert D. M. E. Osterhaus 《Journal of virology》2015,89(11):6131-6135
The ability of Middle East respiratory syndrome coronavirus (MERS-CoV) to infect small animal species may be restricted given the fact that mice, ferrets, and hamsters were shown to resist MERS-CoV infection. We inoculated rabbits with MERS-CoV. Although virus was detected in the lungs, neither significant histopathological changes nor clinical symptoms were observed. Infectious virus, however, was excreted from the upper respiratory tract, indicating a potential route of MERS-CoV transmission in some animal species. 相似文献
35.
V. Stalin Raj Saskia L. Smits Lisette B. Provacia Judith M. A. van den Brand Lidewij Wiersma Werner J. D. Ouwendijk Theo M. Bestebroer Monique I. Spronken Geert van Amerongen Peter J. M. Rottier Ron A. M. Fouchier Berend Jan Bosch Albert D.M.E. Osterhaus Bart L. Haagmans 《Journal of virology》2014,88(3):1834-1838
Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection. 相似文献