全文获取类型
收费全文 | 822篇 |
免费 | 87篇 |
国内免费 | 2篇 |
出版年
2022年 | 3篇 |
2021年 | 12篇 |
2020年 | 5篇 |
2019年 | 9篇 |
2018年 | 11篇 |
2017年 | 9篇 |
2016年 | 15篇 |
2015年 | 31篇 |
2014年 | 40篇 |
2013年 | 51篇 |
2012年 | 58篇 |
2011年 | 51篇 |
2010年 | 32篇 |
2009年 | 30篇 |
2008年 | 55篇 |
2007年 | 47篇 |
2006年 | 44篇 |
2005年 | 38篇 |
2004年 | 57篇 |
2003年 | 40篇 |
2002年 | 41篇 |
2001年 | 11篇 |
2000年 | 6篇 |
1999年 | 16篇 |
1998年 | 24篇 |
1997年 | 4篇 |
1996年 | 6篇 |
1995年 | 8篇 |
1994年 | 10篇 |
1993年 | 15篇 |
1992年 | 10篇 |
1991年 | 6篇 |
1990年 | 6篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 12篇 |
1986年 | 11篇 |
1985年 | 10篇 |
1984年 | 10篇 |
1983年 | 5篇 |
1982年 | 9篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 6篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1970年 | 2篇 |
1969年 | 2篇 |
排序方式: 共有911条查询结果,搜索用时 281 毫秒
841.
Chuan Hao Tan Kai Shyang Koh Chao Xie Martin Tay Yan Zhou Rohan Williams Wun Jern Ng Scott A Rice Staffan Kjelleberg 《The ISME journal》2014,8(6):1186-1197
Quorum sensing (QS) signalling has been extensively studied in single species populations. However, the ecological role of QS in complex, multi-species communities, particularly in the context of community assembly, has neither been experimentally explored nor theoretically addressed. Here, we performed a long-term bioreactor ecology study to address the links between QS, organization and composition of complex microbial communities. The conversion of floccular biomass to highly structured granules was found to be non-random, but strongly and positively correlated with N-acyl-homoserine-lactone (AHL)-mediated QS. Specific AHLs were elevated up to 100-fold and were strongly associated with the initiation of granulation. Similarly, the levels of particular AHLs decreased markedly during the granular disintegration phase. Metadata analysis indicated that granulation was accompanied by changes in extracellular polymeric substance (EPS) production and AHL add-back studies also resulted in increased EPS synthesis. In contrast to the commonly reported nanomolar to micromolar signal concentrations in pure culture laboratory systems, QS signalling in the granulation ecosystem occurred at picomolar to nanomolar concentrations of AHLs. Given that low concentrations of AHLs quantified in this study were sufficient to activate AHL bioreporters in situ in complex granular communities, AHL mediated QS may be a common feature in many natural and engineered ecosystems, where it coordinates community behaviour. 相似文献
842.
Keith W. Larson Miriam Liedvogel BriAnne Addison Oddmund Kleven Terje Laskemoen Jan T. Lifjeld Max Lundberg Susanne ?kesson Staffan Bensch 《PloS one》2014,9(5)
Local adaptation is an important process contributing to population differentiation which can occur in continuous or isolated populations connected by various amounts of gene flow. The willow warbler (Phylloscopus trochilus) is one of the most common songbirds in Fennoscandia. It has a continuous breeding distribution where it is found in all forested habitats from sea level to the tree line and therefore constitutes an ideal species for the study of locally adapted genes associated with environmental gradients. Previous studies in this species identified a genetic marker (AFLP-WW1) that showed a steep north-south cline in central Sweden with one allele associated with coastal lowland habitats and the other with mountainous habitats. It was further demonstrated that this marker is embedded in a highly differentiated chromosome region that spans several megabases. In the present study, we sampled 2,355 individuals at 128 sites across all of Fennoscandia to study the geographic and climatic variables associated with the allele frequency distributions of WW1. Our results demonstrate that 1) allele frequency patterns significantly differ between mountain and lowland populations, 2) these allele differences coincide with extreme temperature conditions and the short growing season in the mountains, and milder conditions in coastal areas, and 3) the northern-allele or “altitude variant” of WW1 occurs in willow warblers that occupy mountainous habitat regardless of subspecies. Finally these results suggest that climate may exert selection on the genomic region associated with these alleles and would allow us to develop testable predictions for the distribution of the genetic marker based on climate change scenarios. 相似文献
843.
Edna J. L. Barbosa Camilla A. M. Glad Anna G. Nilsson Niklas Bosaeus Helena Filipsson Nystr?m Per-Arne Svensson Bengt-?ke Bengtsson Staffan Nilsson Ingvar Bosaeus Cesar Luiz Boguszewski Gudmundur Johannsson 《PloS one》2014,9(8)
Objectives
Growth hormone deficiency (GHD) in adults is associated with decreased extracellular water volume (ECW). In response to GH replacement therapy (GHRT), ECW increases and blood pressure (BP) reduces or remains unchanged. Our primary aim was to study the association between polymorphisms in genes related to renal tubular function with ECW and BP before and 1 year after GHRT. The ECW measures using bioimpedance analysis (BIA) and bioimpedance spectroscopy (BIS) were validated against a reference method, the sodium bromide dilution method (Br−).Design and Methods
Using a candidate gene approach, fifteen single-nucleotide polymorphisms (SNPs) in nine genes with known impact on renal tubular function (AGT, SCNN1A, SCNN1G, SLC12A1, SLC12A3, KCNJ1, STK39, WNK1 and CASR) were genotyped and analyzed for associations with ECW and BP at baseline and with their changes after 1 year of GHRT in 311 adult GHD patients. ECW was measured with the Br−, BIA, and BIS.Results
Both BIA and BIS measurements demonstrated similar ECW results as the reference method. At baseline, after adjustment for sex and BMI, SNP rs2291340 in the SLC12A1 gene was associated with ECW volume in GHD patients (p = 0.039). None of the SNPs influenced the ECW response to GHRT. One SNP in the SLC12A3 gene (rs11643718; p = 0.024) and three SNPs in the SCNN1G gene [rs5723 (p = 0.02), rs5729 (p = 0.016) and rs13331086 (p = 0.035)] were associated with the inter-individual differences in BP levels at baseline. A polymorphism in the calcium-sensing receptor (CASR) gene (rs1965357) was associated with changes in systolic BP after GHRT (p = 0.036). None of these associations remained statistically significant when corrected for multiple testing.Conclusion
The BIA and BIS are as accurate as Br− to measure ECW in GHD adults before and during GHRT. Our study provides the first evidence that individual polymorphisms may have clinically relevant effects on ECW and BP in GHD adults. 相似文献844.
Henrik Tomenius Anna-Karin Pernestig Kristina Jonas Dimitris Georgellis Roland Möllby Staffan Normark Öjar Melefors 《BMC microbiology》2006,6(1):27-8
Background
The Salmonella enterica BarA-SirA, the Erwinia carotovora ExpS-ExpA, the Vibrio cholerae BarA-VarA and the Pseudomonas spp GacS-GacA all belong to the same orthologous family of two-component systems as the Escherichia coli BarA-UvrY. In the first four species it has been demonstrated that disruption of this two-component system leads to a clear reduction in virulence of the bacteria. Our aim was to determine if the Escherichia coli BarA-UvrY two-component system is connected with virulence using a monkey cystitis model. 相似文献845.
Staffan Wallin Leif Hammarstr?m Errol Blart Fabrice Odobel 《Photochemical & photobiological sciences》2006,5(9):828-834
The photophysical and spectroscopic properties of a series of bis-porphyrin compounds meso-meso-linked via oligothiophene bridges are reported. In particular the effects of the different bridges on the porphyrin properties as well as their ability to enhance energy transfer is investigated. The main findings are: a splitting of the degeneracy of the porphyrin Soret band transition with a lower energy transition aligned along the bridge, a dramatic decrease in triplet lifetime and the occurrence of "superexchange" as the main mechanism for mediating singlet-singlet energy transfer in the case where the bridge is a quaterthiophene. Our results show significant perturbations of the intrinsic porphyrin properties induced by the bridge, which are important for the function of porphyrin assemblies. 相似文献
846.
An increasing number of studies describe moving hybrid zones. This raises the issue of their actual frequency and emphasizes the need for methods that enable the detection of zone movements without historical records. Asymmetric introgression, usually considered as a signature of geographical shift, might be misleading when applied to mitochondrial or potentially non-neutral markers. We investigated mitochondrial and genomic introgression, using 30 AFLP derived markers, in a well-documented moving avian contact zone between two warblers. We found no instances of cross-species transmission of mitochondrial DNA but we detected nuclear introgression. Introgression levels were higher in the expanding species. Highest introgression was observed in populations that recently became allopatric than in current sympatric populations, which suggests that alien genetic material mainly spread at the time just before the receding species became extinct. We propose that either local recruitment or positive selection on some loci contribute to this pattern. Furthermore, we propose that, when the rarefaction of sexual partners drive the hybridization process, movement could be revealed by introgression peaking on the rear edge of the moving zone, or in its close vicinity. 相似文献
847.
Environmental impacts of forest production and supply of pulpwood: Spanish and Swedish case studies 总被引:2,自引:0,他引:2
Sara González-García Staffan Berg Gumersindo Feijoo Ma Teresa Moreira 《The International Journal of Life Cycle Assessment》2009,14(4):340-353
Background, aim and scope Forest operations use large amounts of energy, which must be considered when life cycle assessment (LCA) methodology is applied
to forest products. Forest management practices differ considerably between countries and may also differ within a country.
This paper aims to identify and compare the environmental burdens from forest operations in Sweden and Spain focused on pulpwood
production and supply to pulp mills.
Materials and methods To perform the analysis, the main forest plantations were investigated as well as the most important tree species used in
pulp mills in both countries: eucalyptus and, Norway spruce and Scots pine, were taken into account for the Spanish and Swedish
case studies, respectively. Energy requirements for pulpwood production and supply to Spanish and Swedish pulp mills are evaluated
in this paper. All forest operations from site preparation to extraction of felled wood to the delivery point at the roadside
are included within the system boundaries as well as wood transport from forest landing to the pulp mill gate. Seedling and
machinery production are excluded from the system boundaries due to lack of field data. The impact assessment phase was carried
out according to the Swedish Environmental Management Council and, in particular, the impact categories assessed in forest
and agricultural LCAs (global warming, acidification, eutrophication and photochemical oxidant formation) were analysed. SimaPro
7.10 software was used to perform the impact assessment stage.
Results Different types of wood are produced in both case studies: softwood in Sweden and hardwood in Spain, with higher production
of round wood and biomass per hectare in Sweden. Total energy use for pulpwood production and supply are in a similar order
of magnitude, up to 395 MJ and 370 MJ/m3 solid under bark in Spain and Sweden, respectively. Field operations, such as silviculture and logging, are more energy-intensive
in the Spanish case study. However, secondary hauling of pulpwood to pulp mill requires more energy in the Swedish case study.
These important differences are related to different forest management practices as well as to pulpwood supply to the pulp
mill. The eventual imports of pulpwood, application of pesticides, thinning step or final felling considerably affects energy
requirements, which are reflected on the environmental results.
Discussion Although differences between both case studies were observed, several stages were investigated: wood delivery to the pulp
mill by road, harvesting and forwarding, contribute considerably to acidification, eutrophication and global warming potential
in both cases. The type of wood, the machines used in forest operations (mechanised or motor-manual), the use of fossil fuels
and the amount of wood produced influence the results. These differences must be kept in mind in comparative studies between
such different countries.
Conclusions The results obtained in this work allow one to forecast the importance of forest operations in LCA of forest products (in
this case, wood pulp) and the influence of energy use in the results. Special attention has been paid in the inventory analysis
stage for both case studies. It is possible to gain a better environmental performance in both case studies if alternative
practices are considered, mainly focused on site preparation and stand tending in the Spanish system and on pulpwood supply
in the Swedish one.
Recommendations and perspectives This study provides useful information that can assist forest-based industries in the aim of increasing their sustainability.
Future work will focus on the study of several transport alternatives of pulpwood supply including railway, road and ship.
In addition, pulpwood processing in Spanish and Swedish paper pulp mills considered to be representative of the “state of
art” will be carried out in order to get a complete picture of this kind of forest-based industry. In addition, the use of
biofuels (such as forest biomass) instead of fossil fuels and CO2 uptake of wood via photosynthesis will be carried out in order to have a complete perspective of forest ecosystems. 相似文献
848.
Hanne Vihervaara Janne Sundell Hannu Ylönen 《Ethology : formerly Zeitschrift fur Tierpsychologie》2010,116(9):888-894
Infanticide, the killing of conspecific young, is commonly recognized as an adaptive behavioural strategy enhancing the fitness of the perpetrator. Infanticide is supposed to be inhibited in several male rodent species after mating with a time lag to the time when perpetrators own offspring would be born. This is because males with no parental care do not recognize their own offspring. It has been suggested that copulation alone is enough to inhibit infanticidal behaviour in male rodents. Infanticidal behaviour occurs in more than 50% of male bank voles (Myodes glareolus), and offspring loss because of infanticide may have a great effect on breeding success and population recruitment. In a laboratory experiment, we studied whether infanticidal male bank voles after successful mating stop the killing of pups. Infanticidal males were paired with a female until successful copulation. After the young were born, the males’ infanticidal behaviour was studied from the time of expected birth of own pups until their post‐weaning age. We predicted that mated infanticidal males are inhibited from committing infanticide especially during the time period when pups are less than 10 d old. Against our prediction, 67% of the infanticidal males continued the killing of pups in the age of 3 d. Infanticidal behaviour remained stable, and half of the males were infanticidal still at the age of weaning of pups. Our results are contradictory to previous studies, as we observed no inhibition of infanticide during early life of pups nor increase in infanticide again when their own offspring would have reached the ‘safe’ age and size after weaning. We suggest that mating alone is not sufficient to inhibit infanticide. Thus, we suggest that other cues of the female with whom the male mated with or on her territory are needed for inhibition to occur. 相似文献
849.
Staffan Kjelleberg Beverley A. Humphrey Kevin C. Marshall 《Applied microbiology》1982,43(5):1166-1172
The copiotrophic marine Vibrio sp. strain DW1, shown previously in batch culture to increase in numbers at the onset of starvation and then to form viable small cells with low endogenous respiration, appears to have a survival advantage at interfaces. Vibrio sp. strain DW1 behaved differently at interfaces compared with the aqueous phase under starvation conditions: (i) small cells were observed at an air-water interface without nutrients, (ii) nutrients added to the air-water interface quickly produced larger cells at the surface, (iii) motility persisted many hours longer at the solid-water interface of a dialysis membrane in a microchamber at the onset of starvation, and (iv) regrowth and division at the solid-liquid interface occurred quickly and at nutrient concentrations too low to permit growth in the aqueous phase. It was concluded that, if small starved cells from copiotrophic bacteria can reach an interface, additional survival mechanisms become available to them: (i) interfaces constitute areas of favorable nutrient conditions, and (ii) interfaces lacking a sufficient amount of nutrient, nevertheless, trigger cells to become smaller, thus increasing their surface/volume ratio and the packing density. 相似文献
850.
Changes in bacterial surface hydrophobicity, charge, and degree of irreversible binding to glass surfaces of seven marine isolates were followed during starvation. The degree of hydrophobicity was measured by hydrophobic interaction chromatography and by two-phase separation in a hexadecane-water system, whereas changes in charge were measured by electrostatic interaction chromatography. All isolates underwent the starvation-induced responses of fragmentation, which is defined as division without growth, and continuous size reduction, which results in populations with increased numbers of smaller cells. The latter process was also responsible for a significant proportion of the total drop in cell volume; this was observed by noting the biovolume (the average cell multiplied by the number of bacteria) of a population after various times of starvation. Four strains exhibited increases in both hydrophobicity and irreversible binding, initiated after different starvation times. The most hydrophilic and most hydrophobic isolates both showed a small increase in the degree of irreversible binding after only 5 h, followed by a small decrease after 22 h. Their hydrophobicity remained constant, however, throughout the entire starvation period. On the other hand, one strain, EF190, increased its hydrophobicity after 5 h of starvation, although the degree of irreversible binding remained constant. Charge effects could not be generally related to the increase in irreversible binding. Scanning electron micrographs showed a large increase in surface roughness throughout the starvation period for all strains that showed marked changes in physicochemical characteristics. 相似文献