全文获取类型
收费全文 | 921篇 |
免费 | 93篇 |
国内免费 | 32篇 |
出版年
2024年 | 2篇 |
2023年 | 12篇 |
2022年 | 16篇 |
2021年 | 25篇 |
2020年 | 16篇 |
2019年 | 25篇 |
2018年 | 9篇 |
2017年 | 20篇 |
2016年 | 31篇 |
2015年 | 46篇 |
2014年 | 45篇 |
2013年 | 67篇 |
2012年 | 76篇 |
2011年 | 67篇 |
2010年 | 49篇 |
2009年 | 37篇 |
2008年 | 65篇 |
2007年 | 67篇 |
2006年 | 61篇 |
2005年 | 70篇 |
2004年 | 52篇 |
2003年 | 42篇 |
2002年 | 33篇 |
2001年 | 11篇 |
2000年 | 2篇 |
1999年 | 17篇 |
1998年 | 13篇 |
1997年 | 6篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 2篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1986年 | 2篇 |
1982年 | 4篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1976年 | 5篇 |
1972年 | 2篇 |
1969年 | 2篇 |
1961年 | 1篇 |
1955年 | 1篇 |
1954年 | 1篇 |
1952年 | 1篇 |
1949年 | 1篇 |
1947年 | 1篇 |
排序方式: 共有1046条查询结果,搜索用时 250 毫秒
11.
12.
13.
Stacy A. Krueger‐Hadfield Ben A. Flanagan Olivier Godfroy Kristina M. Hill‐Spanik Chris C. Nice Courtney J. Murren Allan E. Strand Erik E. Sotka 《Journal of phycology》2021,57(1):279-294
For many taxa, including isomorphic haplodiplontic macroalgae, determining sex and ploidy is challenging, thereby limiting the scope of some population demographic and genetic studies. Here, we used double‐digest restriction site‐associated DNA sequencing (ddRAD‐seq) to identify sex‐linked molecular markers in the widespread red alga Agarophyton vermiculophyllum. In the ddRAD‐seq library, we included 10 female gametophytes, 10 male gametophytes, and 16 tetrasporophytes from one native and one non‐native site (N = 40 gametophytes and N = 32 tetrasporophytes total). We identified seven putatively female‐linked and 19 putatively male‐linked sequences. Four female‐ and eight male‐linked markers amplified in all three life cycle stages. Using one female‐ and one male‐linked marker that were sex‐specific, we developed a duplex PCR and tested the efficacy of this assay on a subset of thalli sampled at two sites in the non‐native range. We confirmed ploidy based on the visual observation of reproductive structures and previous microsatellite genotyping at 10 polymorphic loci. For 32 vegetative thalli, we were able to assign sex and confirm ploidy in these previously genotyped thalli. These markers will be integral to ongoing studies of A. vermiculophyllum invasion. We discuss the utility of RAD‐seq over other approaches previously used, such as RAPDs (random amplified polymorphic DNA), for future work designing sex‐linked markers in other haplodiplontic macroalgae for which genomes are lacking. 相似文献
14.
15.
Yang Yang Hart Stephen C. McCorkle Emma P. Stacy Erin M. Barnes Morgan E. Hunsaker Carolyn T. Johnson Dale W. Berhe Asmeret Asefaw 《Ecosystems》2021,24(8):1853-1874
Ecosystems - Understanding the transport of dissolved organic carbon (DOC) and nitrogen (N) as water flows through headwater basins is important for predicting downstream water quality. With... 相似文献
16.
17.
Kent Reed Todd Knutson Stacy Krueth Laura Sullivan Lee Chaves 《Animal biotechnology》2013,24(2):81-102
Sequence similarity was used to predict the position of expressed sequence tags (ESTs) in the genome of the turkey (Meleagris gallopavo). Turkey EST sequences were compared with the draft assembly of the chicken whole-genome sequence and the chicken EST database by BLASTN. Among the 877 ESTs examined, 788 had significant matches in the chicken genome sequence. Position of orthologous sequences in the chicken genome and the predicted position of the EST loci in the turkey genome are presented. Genetic assignments suggest a high level of accuracy for the COMPASS predictions. 相似文献
18.
19.
Bradley M. Stacy Kristen K. Comfort Donald A. Comfort Saber M. Hussain 《Plasmonics (Norwell, Mass.)》2013,8(2):1235-1240
Due to their unique plasmonic and optical properties, gold nanorods (GNR) have shown tremendous potential for nano-based applications extending into a variety of fields including bioimaging, sensor development, electronics, and cancer therapy. These distinctive, shape-specific properties are strongly dependent upon the GNR aspect ratio, thus producing the ability to be targeted for an application by fine-tuning their physical parameters. It is owing to their characteristic spectral signature, which is vastly different from that of a cellular setting, that GNRs are emerging as an ideal candidate for nano-based imaging applications. However, one challenge that has emerged in the field of bioimaging is the need to account for the observed plasmon coupling effect that arises from GNR agglomeration in a physiological environment. In this study, GNRs with aspect ratios of 2.5 and 6.0 were actively identified in an in vitro setting through a hyperspectral imaging (HSI) analysis; which successfully recognized and separated the light scattering pattern of these particles from that of the surrounding cells. Through inclusion of agglomerated GNR spectral patterns in the HSI spectral library, this imaging technique was able to overcome the complication of plasmon coupling, though to varying degrees. These results demonstrate the tremendous potential of GNRs coupled with HSI analysis to advance the field of nano-based sensing and imaging mechanisms. 相似文献
20.