全文获取类型
收费全文 | 2003篇 |
免费 | 239篇 |
国内免费 | 1篇 |
出版年
2021年 | 19篇 |
2018年 | 17篇 |
2017年 | 22篇 |
2016年 | 17篇 |
2015年 | 44篇 |
2014年 | 58篇 |
2013年 | 87篇 |
2012年 | 92篇 |
2011年 | 122篇 |
2010年 | 60篇 |
2009年 | 47篇 |
2008年 | 65篇 |
2007年 | 72篇 |
2006年 | 76篇 |
2005年 | 75篇 |
2004年 | 76篇 |
2003年 | 68篇 |
2002年 | 65篇 |
2001年 | 65篇 |
2000年 | 60篇 |
1999年 | 71篇 |
1998年 | 32篇 |
1997年 | 32篇 |
1996年 | 23篇 |
1994年 | 19篇 |
1993年 | 15篇 |
1992年 | 53篇 |
1991年 | 56篇 |
1990年 | 33篇 |
1989年 | 33篇 |
1988年 | 35篇 |
1987年 | 33篇 |
1986年 | 25篇 |
1985年 | 28篇 |
1984年 | 31篇 |
1983年 | 38篇 |
1982年 | 16篇 |
1980年 | 21篇 |
1979年 | 28篇 |
1978年 | 30篇 |
1977年 | 34篇 |
1976年 | 17篇 |
1975年 | 29篇 |
1974年 | 28篇 |
1973年 | 27篇 |
1972年 | 15篇 |
1971年 | 20篇 |
1970年 | 23篇 |
1969年 | 24篇 |
1968年 | 16篇 |
排序方式: 共有2243条查询结果,搜索用时 15 毫秒
161.
We present the foundation for establishing a modular, collaborative, integrated, open-source architecture for image processing of electron microscopy images, named iplt. It is designed around object oriented paradigms and implemented using the programming languages C++ and Python. In many aspects it deviates from classical image processing approaches. This paper intends to motivate developers within the community to participate in this on-going project. The iplt homepage can be found at http://www.iplt.org. 相似文献
162.
163.
Small Maf compound mutants display central nervous system neuronal degeneration,aberrant transcription,and Bach protein mislocalization coincident with myoclonus and abnormal startle response
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Katsuoka F Motohashi H Tamagawa Y Kure S Igarashi K Engel JD Yamamoto M 《Molecular and cellular biology》2003,23(4):1163-1174
164.
Disruption of the pelota gene causes early embryonic lethality and defects in cell cycle progression
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Adham IM Sallam MA Steding G Korabiowska M Brinck U Hoyer-Fender S Oh C Engel W 《Molecular and cellular biology》2003,23(4):1470-1476
Mutations in either the Drosophila melanogaster pelota or pelo gene or the Saccharomyces cerevisiae homologous gene, DOM34, cause defects of spermatogenesis and oogenesis in Drosophila, and delay of growth and failure of sporulation in yeast. These phenotypes suggest that pelota is required for normal progression of the mitotic and meiotic cell cycle. To determine the role of the pelota in mouse development and progression of cell cycle, we have established a targeted disruption of the mouse PELO: Heterozygous animals are variable and fertile. Genotyping of the progeny of heterozygous intercrosses shows the absence of Pelo(-/-) pups and suggests an embryo-lethal phenotype. Histological analyses reveal that the homozygous Pelo deficient embryos fail to develop past day 7.5 of embryogenesis (E7.5). The failure of mitotic active inner cell mass of the Pelo(-/-) blastocysts to expand in growth after 4 days in culture and the survival of mitotic inactive trophoplast indicate that the lethality of Pelo-null embryos is due to defects in cell proliferation. Analysis of the cellular DNA content reveals the significant increase of aneuploid cells in Pelo(-/-) embryos at E7.5. Therefore, the percent increase of aneuploid cells at E7.5 may be directly responsible for the arrested development and suggests that Pelo is required for the maintenance of genomic stability. 相似文献
165.
Akiba Y Furukawa O Guth PH Engel E Nastaskin I Kaunitz JD 《American journal of physiology. Gastrointestinal and liver physiology》2001,280(3):G470-G474
We previously showed that the duodenal hyperemic response to acid occurs through activation of capsaicin-sensitive afferent nerves with subsequent release of vasodilatory substances such as calcitonin gene-related peptide (CGRP) and nitric oxide. We then tested the hypothesis that similar factors regulate duodenal mucus gel thickness. Gel thickness was optically measured using in vivo microscopy in anesthetized rats. Duodenal mucosae were superfused with pH 7.0 buffer with vanilloid receptor agonist capsaicin, bradykinin, or PGE(2) injection or were challenged with pH 2.2 solution, with or without the vanilloid antagonist capsazepine, human CGRP-(8-37), N(G)-nitro-L-arginine methyl ester, and indomethacin. Other rats underwent sensory ablation with high-dose capsaicin pretreatment. Acid, bradykinin, capsaicin, and PGE(2) all quickly thickened the gel. Antagonism of vanilloid and CGRP receptors, inhibition of nitric oxide synthase, and sensory deafferentation delayed gel thickening, suggesting that the capsaicin pathway mediated the initial burst of mucus secretion that thickened the gel. Indomethacin abolished gel thickening due to acid, bradykinin, and capsaicin. Inhibition of gel thickening by indomethacin in response to multiple agonists suggests that cyclooxygenase activity is essential for duodenal gel thickness regulation. Duodenal afferent neural pathways play an important role in the modulation of cyclooxygenase-mediated physiological control of gel thickness. 相似文献
166.
167.
SecYEG assembles into a tetramer to form the active protein translocation channel 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Translocase mediates preprotein translocation across the Escherichia coli inner membrane. It consists of the SecYEG integral membrane protein complex and the peripheral ATPase SecA. Here we show by functional assays, negative-stain electron microscopy and mass measurements with the scanning transmission microscope that SecA recruits SecYEG complexes to form the active translocation channel. The active assembly of SecYEG has a side length of 10.5 nm and exhibits an approximately 5 nm central cavity. The mass and structure of this SecYEG as well as the subunit stoichiometry of SecA and SecY in a soluble translocase-precursor complex reveal that translocase consists of the SecA homodimer and four SecYEG complexes. 相似文献
168.
GATA2 is required for the generation of V2 interneurons 总被引:6,自引:0,他引:6
169.
The cDNAs for human and murine Receptor Activity Modifying Proteins and for the associated murine Calcitonin Receptor Like Receptor were isolated. The human RAMP1 and RAMP3 genes possess two introns and human RAMP2 possesses three introns. Human RAMP1 was assigned to chromosome 2q36-->q37.1, RAMP2 to 17q12-->q21.1 and RAMP3 to 7p13-->p12. Mouse Ramp1 was assigned to chromosome 1 and Ramp2 and Ramp3 were assigned to chromosome 11. 相似文献
170.
A high thermal stability is observed for the five-stranded alpha-helical coiled-coil domain of cartilage oligomeric matrix protein COMP. It does not unfold in non-denaturing buffer between 0 and 100 degrees C and thermal denaturation is only achieved at high concentrations of guanidinium chloride (4-6 M). In these solutions the protein structure is lost at decreasing (cold denaturation) and increasing temperatures (heat denaturation). In the cold denaturation region, the melting profile showed deviations from the theory of Privalov et al. [P.L. Privalov, V. Griko Yu, S. Venyaminov, V.P. Kutyshenko, Cold denaturation of myoglobin, J. Mol. Biol. 190 (1986) 487-498] probably due to deviations from a two-state mechanism. High thermal stability as well as cold and heat denaturation was also observed for a mutant of the coiled-coil domain of COMP in which glutamine 54 was replaced by isoleucine but it still forms pentamer. The melting temperatures in plain buffer for the heat denaturation of COMP coiled-coil domain and its mutant obtained by extrapolation to zero molar guanidinium chloride concentration are approximately 160 and 220 degrees C, respectively, which groups them among the most stable proteins. 相似文献