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Anaerobic, bacterial reduction of water-soluble U(VI) complexes to the poorly soluble U(IV) mineral uraninite has been intensively
studied as a strategy for in situ remediation of uranium-contaminated groundwater. A novel and potentially counteracting metabolic
process, anaerobic, nitrate-dependent U(IV) oxidation, has recently been described in two bacterial species (Geobacter metallireducens and Thiobacillus denitrificans), but the underlying biochemistry and genetics are completely unknown. We report here that two diheme, c-type cytochromes (putatively c
4 and c
5 cytochromes) play a major role in nitrate-dependent U(IV) oxidation by T. denitrificans. Insertion mutations in each of the two genes encoding these cytochromes resulted in a greater than 50% decrease in U(IV)
oxidation activity, and complementation in trans restored activity to wild-type levels. Sucrose-density-gradient ultracentrifugation
confirmed that both cytochromes are membrane-associated. Insertion mutations in genes encoding other membrane-associated,
c-type cytochromes did not diminish U(IV) oxidation. This is the first report of proteins involved in anaerobic U(IV) oxidation.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
72.
Tingfang Chen Alex M. Rohacek Matthew Caporizzo Amir Nankali Jeroen J. Smits Jaap Oostrik Cornelis P. Lanting Erdi Kücük Christian Gilissen Jiddeke M. van de Kamp Ronald J.E. Pennings Staci M. Rakowiecki Klaus H. Kaestner Kevin K. Ohlemiller John S. Oghalai Hannie Kremer Benjamin L. Prosser Douglas J. Epstein 《Developmental cell》2021,56(10):1526-1540.e7
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73.