全文获取类型
收费全文 | 1499篇 |
免费 | 181篇 |
专业分类
1680篇 |
出版年
2023年 | 6篇 |
2022年 | 15篇 |
2021年 | 30篇 |
2020年 | 15篇 |
2019年 | 27篇 |
2018年 | 15篇 |
2017年 | 26篇 |
2016年 | 44篇 |
2015年 | 64篇 |
2014年 | 83篇 |
2013年 | 87篇 |
2012年 | 122篇 |
2011年 | 121篇 |
2010年 | 87篇 |
2009年 | 74篇 |
2008年 | 87篇 |
2007年 | 91篇 |
2006年 | 80篇 |
2005年 | 95篇 |
2004年 | 55篇 |
2003年 | 47篇 |
2002年 | 62篇 |
2001年 | 24篇 |
2000年 | 26篇 |
1999年 | 18篇 |
1998年 | 19篇 |
1997年 | 10篇 |
1996年 | 13篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1992年 | 9篇 |
1991年 | 19篇 |
1990年 | 13篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 15篇 |
1986年 | 8篇 |
1985年 | 5篇 |
1984年 | 14篇 |
1982年 | 7篇 |
1979年 | 6篇 |
1977年 | 6篇 |
1976年 | 9篇 |
1975年 | 5篇 |
1973年 | 5篇 |
1966年 | 4篇 |
1938年 | 3篇 |
1935年 | 3篇 |
1931年 | 3篇 |
1924年 | 3篇 |
排序方式: 共有1680条查询结果,搜索用时 0 毫秒
121.
Qian Jiang Stacey Arnold Tiffany Heanue Krishna?Praneeth Kilambi Betty Doan Ashish Kapoor Albee?Yun Ling Maria?X. Sosa Moltu Guy Qingguang Jiang Grzegorz Burzynski Kristen West Seneca Bessling Paola Griseri Jeanne Amiel Raquel?M. Fernandez Joke?B.G.M. Verheij Robert?M.W. Hofstra Salud Borrego Stanislas Lyonnet Isabella Ceccherini Jeffrey?J. Gray Vassilis Pachnis Andrew?S. McCallion Aravinda Chakravarti 《American journal of human genetics》2015,96(4):581-596
Innervation of the gut is segmentally lost in Hirschsprung disease (HSCR), a consequence of cell-autonomous and non-autonomous defects in enteric neuronal cell differentiation, proliferation, migration, or survival. Rare, high-penetrance coding variants and common, low-penetrance non-coding variants in 13 genes are known to underlie HSCR risk, with the most frequent variants in the ret proto-oncogene (RET). We used a genome-wide association (220 trios) and replication (429 trios) study to reveal a second non-coding variant distal to RET and a non-coding allele on chromosome 7 within the class 3 Semaphorin gene cluster. Analysis in Ret wild-type and Ret-null mice demonstrates specific expression of Sema3a, Sema3c, and Sema3d in the enteric nervous system (ENS). In zebrafish embryos, sema3 knockdowns show reduction of migratory ENS precursors with complete ablation under conjoint ret loss of function. Seven candidate receptors of Sema3 proteins are also expressed within the mouse ENS and their expression is also lost in the ENS of Ret-null embryos. Sequencing of SEMA3A, SEMA3C, and SEMA3D in 254 HSCR-affected subjects followed by in silico protein structure modeling and functional analyses identified five disease-associated alleles with loss-of-function defects in semaphorin dimerization and binding to their cognate neuropilin and plexin receptors. Thus, semaphorin 3C/3D signaling is an evolutionarily conserved regulator of ENS development whose dys-regulation is a cause of enteric aganglionosis. 相似文献
122.
Jana Batovska Mark J. Blacket Karen Brown Stacey E. Lynch 《Ecology and evolution》2016,6(9):3001-3011
DNA barcoding is a modern species identification technique that can be used to distinguish morphologically similar species, and is particularly useful when using small amounts of starting material from partial specimens or from immature stages. In order to use DNA barcoding in a surveillance program, a database containing mosquito barcode sequences is required. This study obtained Cytochrome Oxidase I (COI) sequences for 113 morphologically identified specimens, representing 29 species, six tribes and 12 genera; 17 of these species have not been previously barcoded. Three of the 29 species ─ Culex palpalis, Macleaya macmillani, and an unknown species originally identified as Tripteroides atripes ─ were initially misidentified as they are difficult to separate morphologically, highlighting the utility of DNA barcoding. While most species grouped separately (reciprocally monophyletic), the Cx. pipiens subgroup could not be genetically separated using COI. The average conspecific and congeneric p‐distance was 0.8% and 7.6%, respectively. In our study, we also demonstrate the utility of DNA barcoding in distinguishing exotics from endemic mosquitoes by identifying a single intercepted Stegomyia aegypti egg at an international airport. The use of DNA barcoding dramatically reduced the identification time required compared with rearing specimens through to adults, thereby demonstrating the value of this technique in biosecurity surveillance. The DNA barcodes produced by this study have been uploaded to the ‘Mosquitoes of Australia–Victoria’ project on the Barcode of Life Database (BOLD), which will serve as a resource for the Victorian Arbovirus Disease Control Program and other national and international mosquito surveillance programs. 相似文献
123.
Koichi Miura Kerry M Jacques Stacey Stauffer Atsutaka Kubosaki Kejin Zhu Dianne Snow Hirsch James Resau Yi Zheng Paul A Randazzo 《Molecular cell》2002,9(1):109-119
We have identified ARAP1 and ARAP2 and examined ARAP1 as a possible link between phosphoinositide-, Arf-, and Rho-mediated cell signaling. ARAP1 contains Arf GAP, Rho GAP, Ankyrin repeat, Ras-associating, and five PH domains. In vitro, ARAP1 had Rho GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent Arf GAP activity. ARAP1 associated with the Golgi. The Rho GAP activity mediated cell rounding and loss of stress fibers when ARAP1 was overexpressed. The Arf GAP activity mediated changes in the Golgi apparatus and the formation of filopodia, the latter a consequence of increased cellular activity of Cdc42. The Arf GAP and Rho GAP activities both contributed to inhibiting cell spreading. Thus, ARAP1 is a PIP3-dependent Arf GAP that regulates Arf-, Rho-, and Cdc42-dependent cell activities. 相似文献
124.
Heterothermy in the southern African hedgehog, <Emphasis Type="Italic">Atelerix frontalis</Emphasis>
Hallam SL Mzilikazi N 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2011,181(3):437-445
Most research on mammalian heterothermic responses in southern Africa tends to be laboratory based and biased towards rodents
and smaller members of the Afrotheria. In this study, we continuously measured body temperature of southern African hedgehogs
(Atelerix frontalis) between April and August 2009 (−10°C < T
a < 43°C), kept under semi-captive conditions. A. frontalis showed a high propensity for torpor with animals spending up to 84% of the measurement period torpid. During this study,
A. frontalis displayed the lowest T
b min (ca 1°C) yet recorded in an Afrotropical placental heterotherm. Bout lengths of between 0.7 h (40 min) and 116.3 h (4.8 days)
were recorded. Differences in bout length were observed between lighter individuals compared with an individual exhibiting
a higher body mass at the onset of winter, with low M
b individuals exhibiting daily torpor whereas a heavier individual exhibited torpor bouts that were indicative of hibernation.
Our results suggest that heterothermic responses are an important feature in the energy balance equation of this species and
that body mass at the onset of winter may determine the patterns of heterothermy utilised in this species. 相似文献
125.
Mia Petljak Ludmil B. Alexandrov Jonathan S. Brammeld Stacey Price David C. Wedge Sebastian Grossmann Kevin J. Dawson Young Seok Ju Francesco Iorio Jose M.C. Tubio Ching Chiek Koh Ilias Georgakopoulos-Soares Bernardo Rodríguez–Martín Burçak Otlu Sarah O’Meara Adam P. Butler Andrew Menzies Shriram G. Bhosle Michael R. Stratton 《Cell》2019,176(6):1282-1294.e20
126.
Cobo F Stacey GN Hunt C Cabrera C Nieto A Montes R Cortés JL Catalina P Barnie A Concha A 《Applied microbiology and biotechnology》2005,68(4):456-466
The transplant of cells of human origin is an increasingly complex sector of medicine which entails great opportunities for
the treatment of a range of diseases. Stem cell banks should assure the quality, traceability and safety of cultures for transplantation
and must implement an effective programme to prevent contamination of the final product. In donors, the presence of infectious
micro-organisms, like human immunodeficiency virus, hepatitis B virus, hepatitis C virus and human T cell lymphotrophic virus,
should be evaluated in addition to the possibility of other new infectious agents (e.g. transmissible spongiform encephalopathies
and severe acute respiratory syndrome). The introduction of the nucleic acid amplification can avoid the window period of
these viral infections. Contamination from the laboratory environment can be achieved by routine screening for bacteria, fungi,
yeast and mycoplasma by European pharmacopoeia tests. Fastidious micro-organisms, and an adventitious or endogenous virus,
is a well-known fact that will also have to be considered for processes involving in vitro culture of stem cells. It is also
a standard part of current good practice in stem cell banks to carry out routine environmental microbiological monitoring
of the cleanrooms where the cell cultures and their products are prepared. The risk of viral contamination from products of
animal origin, like bovine serum and mouse fibroblasts as a “feeder layer” for the development of embryonic cell lines, should
also be considered. Stem cell lines should be tested for prion particles and a virus of animal origin that assure an acceptable
quality. 相似文献
127.
Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity 总被引:13,自引:0,他引:13 下载免费PDF全文
Ricci MS Jin Z Dews M Yu D Thomas-Tikhonenko A Dicker DT El-Deiry WS 《Molecular and cellular biology》2004,24(19):8541-8555
128.
The processing of stem cell lines for application in human therapy requires a physical environment in which air quality (i.e., the number of airborne particles) is controlled to minimize risk of contamination. The processing facility should be constructed and operated to minimise the introduction, generation and retention of particles and microorganisms. A formal program of environmental monitoring should be maintained in each stem cell bank to specify and assess key factors and their influence on the microbiological quality of the process and product. This program should assure the manipulation of cells involved in the derivation of stem cell lines and their culture under established limits for airborne particles and for microbial contamination of the air and surfaces. Environmental monitoring should also address the regulatory requirements in the countries in which the cells will be used. The monitoring programme will depend on local conditions in each processing centre or cell bank. Each centre will need to evaluate its specific needs and establish appropriate monitoring procedures which should not become intrusive to the extent that they might compromise the quality of the cell banks or products. 相似文献
129.
Emily H. M. Wong Chun-Laam Ng Vincent Chi-Hang Lui Man-ting So Stacey S. Cherny Pak-Chung Sham Paul Kwong-Hang Tam Maria-Mercè Garcia-Barceló 《PloS one》2013,8(8)
Anorectal malformations (ARMs) are birth defects that require surgery and carry significant chronic morbidity. Our earlier genome-wide copy number variation (CNV) study had provided a wealth of candidate loci. To find out whether these candidate loci are related to important developmental pathways, we have performed an extensive literature search coupled with the currently available bioinformatics tools. This has allowed us to assign both genic and non-genic CNVs to interrelated pathways known to govern the development of the anorectal region. We have linked 11 candidate genes to the WNT signalling pathway and 17 genes to the cytoskeletal network. Interestingly, candidate genes with similar functions are disrupted by the same type of CNV. The gene network we discovered provides evidence that rare mutations in different interrelated genes may lead to similar phenotypes, accounting for genetic heterogeneity in ARMs. Classification of patients according to the affected pathway and lesion type should eventually improve the diagnosis and the identification of common genes/molecules as therapeutic targets. 相似文献