全文获取类型
收费全文 | 309篇 |
免费 | 15篇 |
专业分类
324篇 |
出版年
2023年 | 4篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 13篇 |
2018年 | 4篇 |
2017年 | 13篇 |
2016年 | 15篇 |
2015年 | 13篇 |
2014年 | 25篇 |
2013年 | 31篇 |
2012年 | 31篇 |
2011年 | 23篇 |
2010年 | 18篇 |
2009年 | 21篇 |
2008年 | 21篇 |
2007年 | 10篇 |
2006年 | 8篇 |
2005年 | 9篇 |
2004年 | 19篇 |
2003年 | 4篇 |
2002年 | 11篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
排序方式: 共有324条查询结果,搜索用时 0 毫秒
321.
S. Katla B. Karmakar S.R.R. Tadi N. Mohan B. Anand U. Pal S. Sivaprakasam 《Journal of applied microbiology》2019,126(5):1438-1453
322.
A high defibrillation threshold occurs in approximately 6% of implants. The defibrillation threshold can be improved by addition of a defibrillation lead. However, the DF-4 high energy ICD header precludes the addition of a defibrillation lead. Here we report on use of a new high voltage adaptor/splitter that enables the addition of an extra defibrillation lead. 相似文献
323.
Michael E.B. FitzPatrick Nicholas M. Provine Lucy C. Garner Kate Powell Ali Amini Sophie L. Irwin Helen Ferry Tim Ambrose Peter Friend Georgios Vrakas Srikanth Reddy Elizabeth Soilleux Paul Klenerman Philip J. Allan 《Cell reports》2021,34(3):108661
- Download : Download high-res image (154KB)
- Download : Download full-size image
324.
Phillip Grant Barbara Ahlemeyer Srikanth Karnati Timm Berg Ingra Stelzig Anca Nenicu Klaus Kuchelmeister Denis I. Crane Eveline Baumgart-Vogt 《Histochemistry and cell biology》2013,140(4):423-442
Catalase and ABCD3 are frequently used as markers for the localization of peroxisomes in morphological experiments. Their abundance, however, is highly dependent on metabolic demands, reducing the validity of analyses of peroxisomal abundance and distribution based solely on these proteins. We therefore attempted to find a protein which can be used as an optimal marker for peroxisomes in a variety of species, tissues, cell types and also experimental designs, independently of peroxisomal metabolism. We found that the biogenesis protein peroxin 14 (PEX14) is present in comparable amounts in the membranes of every peroxisome and is optimally suited for immunoblotting, immunohistochemistry, immunofluorescence, and immunoelectron microscopy. Using antibodies against PEX14, we could visualize peroxisomes with almost undetectable catalase content in various mammalian tissue sections (submandibular and adrenal gland, kidney, testis, ovary, brain, and pancreas from mouse, cat, baboon, and human) and cell cultures (primary cells and cell lines). Peroxisome labeling with catalase often showed a similar tissue distribution to the mitochondrial enzyme mitochondrial superoxide dismutase (both responsible for the degradation of reactive oxygen species), whereas ABCD3 exhibited a distinct labeling only in cells involved in lipid metabolism. We increased the sensitivity of our methods by using QuantumDots?, which have higher emission yields compared to classic fluorochromes and are unsusceptible to photobleaching, thereby allowing more exact quantification without artificial mistakes due to heterogeneity of individual peroxisomes. We conclude that PEX14 is indeed the best marker for labeling of peroxisomes in a variety of tissues and cell types in a consistent fashion for comparative morphometry. 相似文献