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311.
Different purified proteins were shown to give purple formazan bands corresponding to the protein stain following electrophoresis on polyacrylamide gels, in the presence of nitrobluetetrazolium (NBT) and phenazine methosulfate (PMS). Both PMS and NBT are needed for formazan production which has a favorable pH at 8.5. Sulfhydryl blockers in the incubation medium inhibited this color development to different extents. While proteins with free SH groups like bovine serum albumin, ovalbumin, and urease showed this pyridine nucleotide independent artifact, nonthiol proteins, viz., bovine pancreatic ribonuclease A, and riboflavin-binding protein from chicken egg white failed to do so. The nonenzymatic formazan formation observed with different proteins could also be shown in an in vitro assay system. It is clear that the “nothing dehydrogenase” phenomenon observed in several cases may be due to the thiol group-mediated artifactual staining of proteins. 相似文献
312.
Filsy Samuel William P. Flavin Sobia Iqbal Consiglia Pacelli Sri Dushyaanthan Sri Renganathan Louis-Eric Trudeau Edward M. Campbell Paul E. Fraser Anurag Tandon 《The Journal of biological chemistry》2016,291(9):4374-4385
Although trace levels of phosphorylated α-synuclein (α-syn) are detectable in normal brains, nearly all α-syn accumulated within Lewy bodies in Parkinson disease brains is phosphorylated on serine 129 (Ser-129). The role of the phosphoserine residue and its effects on α-syn structure, function, and intracellular accumulation are poorly understood. Here, co-expression of α-syn and polo-like kinase 2 (PLK2), a kinase that targets Ser-129, was used to generate phosphorylated α-syn for biophysical and biological characterization. Misfolding and fibril formation of phosphorylated α-syn isoforms were detected earlier, although the fibrils remained phosphatase- and protease-sensitive. Membrane binding of α-syn monomers was differentially affected by phosphorylation depending on the Parkinson disease-linked mutation. WT α-syn binding to presynaptic membranes was not affected by phosphorylation, whereas A30P α-syn binding was greatly increased, and A53T α-syn was slightly lower, implicating distal effects of the carboxyl- on amino-terminal membrane binding. Endocytic vesicle-mediated internalization of pre-formed fibrils into non-neuronal cells and dopaminergic neurons matched the efficacy of α-syn membrane binding. Finally, the disruption of internalized vesicle membranes was enhanced by the phosphorylated α-syn isoforms, a potential means for misfolded extracellular or lumenal α-syn to access cytosolic α-syn. Our results suggest that the threshold for vesicle permeabilization is evident even at low levels of α-syn internalization and are relevant to therapeutic strategies to reduce intercellular propagation of α-syn misfolding. 相似文献
313.
Meignana Arumugham Indiran Aravind Kumar Subramanian Jayashri Prabakar R Pradeep Kumar D Sri Sakthi L Leelavathi 《Bioinformation》2020,16(12):974
It is of interest to document data on oral health care services for adults with cognitive and intellectual disabilities. Hence, a study protocol was registered at the International Prospective Register of Systematic Reviews (PROSPERO) with registration number: CRD42020150759. We used PubMed, Science Direct, LILACS and SCIELO to collect data from known literature using keywords containing MESH (Medical Subject Headings) terms. The risk of bias rating for the collected data was calculated using the Newcastle-Ottawa assessment Scale. The AHRQ (Agency for Healthcare, Research and Quality) was used for classifying the level of evidence in the collected data. Analysis of available data shows that there is a lack of dentists with adequate skills to treat people with disabilities resulting in high cost for dental treatment. Thus, we conclude that inconvenient location of dental clinic, lack of dentists willing to treat people with disabilities and attitude of dental staff towards people with learning disabilities were considered as barriers and challenges faced for dental health service utilization in this context. 相似文献
314.
Chandrabose Karthikeyan Pramod Jharia Digambar Kumar Waiker Amy Catherine Nusbaum Haneen Amawi Erin Marie Kirwen Ryann Christman Sri Krishna Chaitanya Arudra Laurent Meijer Amit K. Tiwari Piyush Trivedi 《Bioorganic & medicinal chemistry letters》2017,27(12):2663-2667
Described herein is the design, synthesis and biological evaluation of a series of N-(1H-pyrazol-3-yl)quinazolin-4-amines against a panel of eight disease relevant protein kinases. The kinase inhibition results indicated that two compounds inhibited casein kinase 1δ/ε (CK1δ/ε) with some selectivity over related kinases, namely CDK5/p25, GSK-3α/β, and DYRK1A. Docking studies with 3c and 3d revealed the key interactions with desired amino acids in the ATP binding site of CK1δ. Furthermore, compound 3c also elicited selective cytotoxic activity against the pancreas ductal adenocarcinoma (PANC-1) cell line. Taken together, the results of this study establish N-(1H-pyrazol-3-yl)quinazolin-4-amines especially 3c and 3d as valuable lead molecules with great potential for CK1δ/ε inhibitor development targeting neurodegenerative disorders and cancer. 相似文献
315.
We have cloned the cDNA coding the beta-tropomyosin of human muscle in an expression vector whose expression depends upon a promotor that can be induced by isopropyl-beta-thiogalactopyranoside. We show that a new protein was synthesized by bacteria containing the engineered plasmid. This protein was heat stable and reacted with antibodies against tropomyosin. We have purified this protein and further identified it by determining its amino acid composition and sequencing the NH2 terminal. Unlike the native muscle tropomyosin, the NH2 terminal is not acetylated and contains a methionine. The circular dichroism spectrum is compatible with 100% alpha-helices. These results show that the protein synthesized in E. coli possesses a native structure. 相似文献
316.
Sri Devi Sukumaran Shah Bakhtiar Nasir Jia Ti Tee Michael J. C. Buckle Rozana Othman Noorsaadah Abd. Rahman Vannajan Sanghiran Lee Syed Nasir Abbas Bukhari Chin Fei Chee 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):130
A series of C4-substituted tertiary nitrogen-bearing 2′-hydroxychalcones were designed and synthesised based on a previous mixed type acetylcholinesterase inhibitor. Majority of the 2′-hydroxychalcone analogues displayed a better inhibition against acetylcholinesterase (AChE) than butyrylcholinesterase (BuChE). Among them, compound 4c was identified as the most potent AChE inhibitor (IC50: 3.3 µM) and showed the highest selectivity for AChE over BuChE (ratio >30:1). Molecular docking studies suggested that compound 4c interacts with both the peripheral anionic site (PAS) and catalytic anionic site (CAS) regions of AChE. ADMET analysis confirmed the therapeutic potential of compound 4c based on its blood–brain barrier penetrating. Overall, the results suggest that this 2′-hydroxychalcone deserves further investigation into the therapeutic lead for Alzheimer’s disease (AD). 相似文献
317.
Issac Praveen Kumar Guru Ajay Chandrakumar Sri Snehaa Lite Christy Saraswathi N. T. Arasu Mariadhas Valan Al-Dhabi Naif Abdullah Arshad Aziz Arockiaraj Jesu 《Molecular biology reports》2020,47(9):6727-6740
Molecular Biology Reports - Understanding the mechanism by which the exogenous biomolecule modulates the GLUT-4 signalling cascade along with the information on glucose metabolism is essential for... 相似文献
318.
Stefania Pagliari Vladimir Vinarsky Fabiana Martino Ana Rubina Perestrelo Jorge Oliver De La Cruz Guido Caluori Jan Vrbsky Pamela Mozetic Antonio Pompeiano Andrea Zancla Sri Ganji Ranjani Petr Skladal Dan Kytyr Zbynk Zdrhal Gabriele Grassi Maurilio Sampaolesi Alberto Rainer Giancarlo Forte 《Cell death and differentiation》2021,28(4):1193
319.
Background
Sequence similarity between proteins is usually considered a reliable indicator of homology. Pyruvate-ferredoxin oxidoreductase and quinol-fumarate reductase contain ferredoxin domains that bind [Fe-S] clusters and are involved in electron transport. Profile-based methods for sequence comparison, such as PSI-BLAST and HMMer, suggest statistically significant similarity between these domains. 相似文献320.
D. Ballerini D. Parlouar M. Lapeyronnie K. Sri 《Applied microbiology and biotechnology》1977,4(1):1-9
Summary A mixed culture of bacteria MS1 capable of growth on methanol as its sole carbon-energy source was isolated by continuous culture with a minimum mineral salts medium and without asepsis. This bacterial association is composed of four Gram-negative bacterial strains which we identified. The respective numerical proportions of each microorganism are stable in time. The properties, and specially the stability of the continuous culture process have been studied with respect to substrate yield, biomass and productivity. 相似文献