Development of the embryonic mammalian pancreas: The relationship between morphogenesis and cytodifferentiation

The degree to which cytodifferentiation is obligatorily coupled to ongoing morphogenesis has been analyzed in the developing mouse pancreas system. Protodifferentiated pancreatic epithelia are isolated from investing mesoderm and are cultured on plastic substrata. Within 24 hr, the epithelia spread into two-dimensional monolayers. Under these conditions, acinar formation fails to take place. Furthermore, the cells do not undergo mitosis, an observation consistent with the absence of mesenchymal cells or mesenchymal factor from these cultures. In spite of the absence of morphogenesis, cytodifferentiation takes place on schedule. Thus, over 5–7 days of culture, amylase specific activity rises 100-fold, and zymogen granules appear in the cells. The results argue that the regulation of exocrine cytodifferentiation is independent of ongoing morphogenesis.     

Growth and morphogenesis of embryonic mouse organs on biopore membrane

Research supported by the American Heart Association, the Wesley Foundation, and NASA (NAGW 1197 and 2328).     

Further studies on bovine serum amylase--the effect of gel buffer pH.

This paper describes the effect of gel buffer pH on the resolution of bovine serum amylase (Amylase I) isozymes in starch gel and the consequences for the understanding of the genetics of this locus. The two main findings are: (1) the existence of a satellite isozyme E to isozyme C which at pH 7.3 has the same mobility as the B isozyme but which at pH 8.0 migrates slower than B, and (2) the finding of three alleles Aml A, Aml B and Aml C in British cattle populations previously reported as having only Aml B and Aml C.