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51.
Mihaela C. Balasu Laurentiu N. Spiridon Simona Miron Constantin T. Craescu Axel J. Scheidig Andrei-Jose Petrescu Stefan E. Szedlacsek 《PloS one》2009,4(5)
The activity of ERK2, an essential component of MAP-kinase pathway, is under the strict control of various effector proteins. Despite numerous efforts, no crystal structure of ERK2 complexed with such partners has been obtained so far. PTP-SL is a major regulator of ERK2 activity. To investigate the ERK2–PTP-SL complex we used a combined method based on cross-linking, MALDI-TOF analysis, isothermal titration calorimetry, molecular modeling and docking. Hence, new insights into the stoichiometry, thermodynamics and interacting regions of the complex are obtained and a structural model of ERK2-PTP-SL complex in a state consistent with PTP-SL phosphatase activity is developed incorporating all the experimental constraints available at hand to date. According to this model, part of the N-terminal region of PTP-SL has propensity for intrinsic disorder and becomes structured within the complex with ERK2. The proposed model accounts for the structural basis of several experimental findings such as the complex-dissociating effect of ATP, or PTP-SL blocking effect on the ERK2 export to the nucleus. A general observation emerging from this model is that regions involved in substrate binding in PTP-SL and ERK2, respectively are interacting within the interface of the complex. 相似文献
52.
Spiridon Papapetropoulos Heather Katzen Anette Schrag Carlos Singer Blake K Scanlon Daniel Nation Alexandra Guevara Bonnie Levin 《BMC neurology》2008,8(1):21