排序方式: 共有55条查询结果,搜索用时 15 毫秒
21.
D. Habedank I. Reindl G. Vietzke U. Bauer A. Sperfeld S. Gl?ser K. D. Wernecke F. X. Kleber 《European journal of applied physiology and occupational physiology》1998,77(5):421-426
The ventilatory equivalent for CO2 defines ventilatory efficiency largely independent of metabolism. An impairment of ventilatory efficiency may be caused by
an increase in either anatomical or physiological dead space, the latter being the most important mechanism in the hyperpnoea
of heart failure, pulmonary embolism, pulmonary hypertension and the former in restrictive lung disease. However, normal values
for ventilatory efficiency have not yet been established. We investigated 101 (56 men) healthy volunteers, aged 16–75 years,
measuring ventilation and gas exchange at rest (n = 64) and on exercise (modified Naughton protocol, n = 101). Age and sex dependent normal values for ventilatory efficiency at rest defined as the ratio ventilation:carbon dioxide
output (V˙
E:V˙CO2), exercise ventilatory efficiency during exercise, defined as the slope of the linear relationship between ventilation and
carbon dioxide output (V˙
E vs V˙CO2 slope), oxygen uptake at the anaerobic threshold and at maximum (V˙O2AT,V˙O2max, respectively) and breathing reserve were established. Ventilatory efficiency at rest was largely independent of age, but
was smaller in the men than in the women [V˙
E:V˙CO2 50.5 (SD 8.8) vs 57.6 (SD 12.6) P<0.05]. Ventilatory efficiency during exercise declined significantly with age and was smaller in the men than in the women
(men: (V˙
E vs V˙CO2 slope = 0.13 × age + 19.9; women: V˙
E vs V˙CO2 slope = 0.12 × age + 24.4). The V˙O2AT and V˙O2max were 23 (SD 5) and 39 (SD 7) ml O2 · kg · min−1 in the men and 18 (SD 4) and 32 (SD 7) in the women, respectively, and declined significantly with age. The V˙O2AT was reached at 58 (SD 9)% V˙O2max. Breathing reserve at the end of exercise was 41% and was independent of sex and age. It was concluded from this study that
ventilatory efficiency as well as peak oxygen uptake are age and sex dependent in adults.
Accepted: 11 June 1997 相似文献
22.
Ca2+, annexins, and GTP modulate exocytosis from maize root cap protoplasts 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《The Plant cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Protoplasts isolated from root cap cells of maize were shown to secrete fucose-rich polysaccharides and were used in a patch-clamp study to monitor changes in whole-cell capacitance. Ca2+ was required for exocytosis, which was measured as an increase in cell capacitance during intracellular dialysis with Ca2+ buffers via the patch pipette. Exocytosis was stimulated significantly by small increases above normal resting [Ca2+]. In the absence of Ca2+, protoplasts decreased in size. In situ hybridization showed significant expression of the maize annexin p35 in root cap cells, differ-entiating vascular tissue, and elongating cells. Dialysis of protoplasts with maize annexins stimulated exocytosis at physiological [Ca2+], and this could be blocked by dialysis with antibodies specific to maize annexins. Dialysis with milli-molar concentrations of GTP strongly inhibited exocytosis, causing protoplasts to decrease in size. GTPgammaS and GDPbetaS both caused only a slight inhibition of exocytosis at physiological Ca2+. Protoplasts were shown to internalize plasma membrane actively. The results are discussed in relation to the regulation of exocytosis in what is usually considered to be a constitutively secreting system; they provide direct evidence for a role of annexins in exocytosis in plant cells. 相似文献
23.
CEES GRASHOFF † PAUL DIJKSTRA † SANDERINE NONHEBEL ‡ AD H.C.M. SCHAPENDONK† SIEBE C. VAN DE GEIJN† 《Global Change Biology》1995,1(6):417-428
The effect of elevated [CO2] on the productivity of spring wheat, winter wheat and faba bean was studied in experiments in climatized crop enclosures in the Wageningen Rhizolab in 1991–93. Simulation models for crop growth were used to explore possible causes for the observed differences in the CO2 response. Measurements of the canopy gas exchange (CO2 and water vapour) were made continuously from emergence until harvest. At an external [CO2] of 700 μmol mol?1 Maximum Canopy CO2 Exchange Rate (CCERmax) at canopy closure was stimulated by 51% for spring wheat and by 71% for faba bean. At the end of the growing season, above ground biomass increase at 700 μmol mol?1 was 58% (faba bean), 35% (spring wheat) and 19% (winter wheat) and the harvest index did not change. For model exploration, weather data sets for the period 1975-88 and 1991–93 were used, assuming adequate water supply and [CO2] at 350 and 700 μmol mol?1. For spring wheat the simulated responses (35–50%) were at the upper end of the experimental results. In agreement with experiments, simulations showed smaller responses for winter wheat and larger responses for faba bean. Further model explorations showed that this differential effect in the CO2 response may not be primarily due to fundamental physiological differences between the crops, but may be at least partly due to differences in the daily air temperatures during comparable stages of growth of these crops. Simulations also showed that variations between years in CO2 response can be largely explained by differences in weather conditions (especially temperature) between growing seasons. 相似文献
24.
Sigurd D. Süssmuth Anne D. Sperfeld Albert C. Ludolph Hayrettin Tumani 《Neurochemical research》2010,35(7):1071-1074
Elevated cerebrospinal fluid (CSF)/serum quotients of albumin (QAlb) may occur in motor neuron diseases (MND) including amyotrophic lateral sclerosis (ALS), but the pathophysiologic mechanisms
underlying these alterations are unclear. Evidence from animal experiments suggests that the arterial carbon dioxide level
might affect the QAlb, i.e. the function of the blood-CSF barrier (BCB). We therefore compared basic CSF parameters in different forms of MND (ALS,
n = 105; lower motor neuron diseases, n = 12; and upper motor neuron diseases, n = 7) and investigated the relationship between elevated QAlb and the arterial partial pressure of carbon dioxide (pCO2) in ALS where respiratory insufficiency leads to hypercapnia in the course of the disease. Pathologic elevations of QAlb occurred in 32 of 124 MND patients. In ALS, QAlb significantly correlated with the arterial pCO2 (r = 0.454; P = 0.001; n = 45). These data indicate that BCB dysfunction is a frequent finding in different forms of MND and may reflect distinct
pathophysiological mechanisms. In ALS, an important underlying mechanism might be the influence of the arterial pCO2 which may alter the CSF flow. 相似文献
25.
26.
27.
Abstract: The Spanish town of Galve (Teruel) is notable because of the abundance of Upper Jurassic and, especially, Lower Cretaceous vertebrates recorded there. Although most groups have been studied in detail, information on turtles is very limited even though the material is relatively abundant. So far, no turtle taxa have been identified at the generic level. The only Lower Cretaceous articulated specimen from Galve is analysed here. It is identified as a representative of Cryptodira, Galvechelone lopezmartinezae gen. et sp. nov. Galvechelone lopezmartinezae is determined as a taxon belonging to the node that groups the turtles traditionally assigned to ‘Macrobaenidae’ and ‘Sinemydidae’, and other taxa such as the members of Panchelonioidea. This node, very abundant in the Lower Cretaceous of Asia, and with a broad subsequent distribution, has recently been recognized in the Lower Cretaceous of Europe. The diversity of basal members of Eucryptodira in the European Late Jurassic (represented by Thalassemydidae, Plesiochelyidae and Eurysternidae) was high. Owing to a relative scarcity of well‐preserved early Cretaceous turtles from Europe, the knowledge of this group of reptiles is limited. The study of the new turtle from Galve, together with the recently described Hoyasemys jimenezi, and the recently completed review of the enigmatic Chitracephalus dumonii demonstrate that members of the cryptodiran node grouping ‘Macrobaenidae’, ‘Sinemydidae’ and Panchelonioidea were also very diverse in this period. 相似文献
28.
Alexander F. Keist Florentina Ferchiu Lea Sante Sebastian Frese Wilhelm Bloch Florian Kreppel Stefan Kochanek Anca Sindrilaru Sebastian Iben Josef Högel Michael Ohnmacht Lutz E. Claes Anita Ignatius Jin Ho Chung Min Jung Lee York Kamenisch Mark Berneburg Thorsten Nikolaus Kerstin E. Braunstein Anne‐Dorte Sperfeld Albert C. Ludolph Karlis Briviba Meinhard Wlaschek Lore Florin Peter Angel Karin Scharffetter‐Kochanek 《Aging cell》2011,10(5):912-912
29.
Corrinoid cofactors play a crucial role as methyl group carriers in the C1 metabolism of anaerobes, e.g. in the cleavage of phenyl methyl ethers by O‐demethylases. For the methylation, the protein‐bound corrinoid has to be in the super‐reduced [CoI]‐state, which is highly sensitive to autoxidation. The reduction of inadvertently oxidized corrinoids ([CoII]‐state) is catalysed in an ATP‐dependent reaction by RACE proteins, the r eductive a ctivators of c orrinoid‐dependent e nzymes. In this study, a reductive activator of O‐demethylase corrinoid proteins was characterized with respect to its ATPase and corrinoid reduction activity. The reduction of the corrinoid cofactor was dependent on the presence of potassium or ammonium ions. In the absence of the corrinoid protein, a basal slow ATP hydrolysis was observed which was obviously not coupled to corrinoid reduction. ATP hydrolysis was significantly stimulated by the corrinoid protein in the [CoII]‐state of the corrinoid cofactor. The stoichiometry of ATP hydrolysed per mol corrinoid reduced was near 1:1. Site‐directed mutagenesis was applied to study the impact of a highly conserved region possibly involved in nucleotide binding of RACE proteins, indicating that an aspartate and a glycine residue may play an essential role for the function of the enzyme. 相似文献
30.
Ecology of predator-induced morphological defense traits in Daphnia longispina (Cladocera,Arthropoda) 总被引:1,自引:0,他引:1
Sperfeld Erik Nilssen Jens Petter Rinehart Shelby Schwenk Klaus Hessen Dag Olav 《Oecologia》2020,192(3):687-698
Oecologia - Inducible defenses against predators are widespread among plants and animals. For example, some Daphnia species form neckteeth against predatory larvae of the dipteran genus Chaoborus.... 相似文献