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81.
Four patients with peripheral neuropathy and nonmalignant monoclonal gammopathy with anti-myelin-associated glycoprotein (MAG) antibodies were studied to determine whether secretion of anti-MAG IgM antibodies by B cells was autonomous, or whether the monoclonal B cells were responsive to T cells. Secretion of anti-MAG IgM by isolated B cells was stimulated by the addition of increasing numbers of pokeweed mitogen (PWM)-activated autologous OKT4+ helper T cells in all four patients. Secretion of anti-MAG IgM by peripheral blood lymphocytes was dependent on the ratio of OKT4+ T helper cells to OKT8+ T suppressor/cytotoxic cells. In three patients with an OKT4+ to OKT8+ T-cell ratio of 2:1, PWM activation stimulated secretion of anti-MAG IgM; in one patient with an OKT4+ to OKT8+ ratio of 1:2, activation by PWM suppressed anti-MAG IgM secretion. These studies suggest that the monoclonal B cells that secrete anti-MAG IgM are responsive to regulatory T cells. 相似文献
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Cerebrovascular Endothelial Cell Culture: Metabolism and Synthesis of 5-Hydroxytryptamine 总被引:3,自引:2,他引:1
The presence of 5-hydroxytryptamine was investigated in cultured and propagated cerebrovascular endothelium using immunohistochemistry and high pressure liquid chromatography. These studies demonstrate that the endothelium has the ability to take up and metabolize 5-hydroxytryptamine as well as to synthesize this amine from its precursor L-tryptophan, thus providing evidence for extraneural synthesis of 5-hydroxytryptamine in the central nervous system. 相似文献
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One‐year outcomes of an intense workplace cardio‐metabolic risk reduction program among high‐risk employees: The My Unlimited Potential
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Henry Guzman Sankalp Das Doris Brown Chukwuemeka U. Osondu Erica Spatz Brandon Shaffer Joann Santiago‐Charles Teresa Ochoa Joseph Mora Cynthia Gilliam Virginia Lehn Shoshana Sherriff Thinh H. Tran Janisse Post Emir Veledar Theodore Feldman Arthur S. Agatston Khurram Nasir 《Obesity (Silver Spring, Md.)》2016,24(1):71-78
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Visual learning in Drosophila populations is characterized by a fast rate of acquisition, and conditioning indices near 0.3 are obtained. Heterogeneity of the populations with respect to this behaviour is small. Reversed conditioning is possible. Prolonged extinction procedures fail to reduce the conditioning index to zero. Memory lasts for a minimum of 14 hours. 相似文献
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Asymmetric transcription of SPP1 in vivo 总被引:5,自引:0,他引:5
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Angela CM Luyf Barbera DC van Schaik Michel de Vries Frank Baas Antoine HC van Kampen Silvia D Olabarriaga 《BMC bioinformatics》2010,11(1):598
Background
Bioinformatics is confronted with a new data explosion due to the availability of high throughput DNA sequencers. Data storage and analysis becomes a problem on local servers, and therefore it is needed to switch to other IT infrastructures. Grid and workflow technology can help to handle the data more efficiently, as well as facilitate collaborations. However, interfaces to grids are often unfriendly to novice users. 相似文献90.