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排序方式: 共有252条查询结果,搜索用时 31 毫秒
91.
Pravesh Kumar Bundhun Akash Bhurtu Mohammad Zafooruddin Sani Soogund Man-Yun Long 《PloS one》2016,11(4)
BackgroundSeveral studies have shown Drug Eluting Stents (DES) to be better compared to Bare Metal Stents (BMS) in patients with type 2 Diabetes Mellitus (T2DM). Since, the adverse clinical outcomes in patients with Insulin-Treated Type 2 Diabetes Mellitus (ITDM) implanted with DES and BMS have not been previously studied, we aim to compare the clinical outcomes in similar patients with cardiovascular diseases, treated with DES and BMS.MethodsRandomized Controlled Trials (RCTs) comparing patients treated with DES and BMS were searched from PubMed and EMBASE databases. Outcome data for the patients with ITDM were carefully extracted. Major Adverse Cardiac Events (MACEs), mortality, Target Vessel Revascularization (TVR), Target Lesion Revascularization (TLR), Myocardial Infarction (MI) and Stent Thrombosis (ST) were considered as the clinical endpoints for this analysis. Odds ratios (OR) with 95% confidence intervals (CIs) were calculated and the pooled analyses were performed with RevMan 5.3 software.ResultsTen RCTs consisting of 830 patients with ITDM (477 patients in the DES group and 353 patients in the BMS group) from a total number of 9,141 patients were included in this analysis. During a follow-up period from one month to one year, MACEs were not increased with the use of DES in these patients with ITDM. At 9 months, MACEs were significantly lower in the DES group with OR: 0.40, 95% CI: 0.23–0.72; P = 0.002 with no increase in mortality. TVR and TLR also favored the DES group with OR: 0.44, 95% CI: 0.22–0.88, P = 0.02 and OR: 0.28, 95% CI: 0.14–0.53; P = 0.0001 respectively at 9 months, and OR: 0.46, 95% CI: 0.23–0.94, P = 0.03 and OR: 0.28, 95% CI: 0.14–0.55; P = 0.0003 respectively at one year. Results for MI, and ST were not statistically significant.ConclusionCompared to BMS, DES were associated with a significantly lower rate of repeated revascularization, without any increase in MACEs or mortality in these patients with ITDM during a follow up period of one year. However, due to the very small population size, further studies with a larger number of randomized patients are required to completely solve this issue. 相似文献
92.
Renata Stripecke Yoko B. Rosato Spartaco Astolfi-Filho 《Applied microbiology and biotechnology》1989,31(5-6):512-517
93.
Andrew J. Nok Paul C. Onyenekwe Sani Ibrahim Asabe Bature Emmanuel Ogbadoyi 《Cell biochemistry and function》1995,13(2):149-151
The activity of glutathione reductase (GHSR) in extracts of kidney, liver and testis of rats infected with Trypanosoma congolense decreased with every wave of parasitemia. The implications of these observations as they relate to the risk of oxidative stress are discussed. 相似文献
94.
Lack of correlation between fibrinolysis and the transformed state of cultured mammalian cells 总被引:9,自引:0,他引:9
D M Mott P H Fabisch B P Sani S Sorof 《Biochemical and biophysical research communications》1974,61(2):621-627
Transformed cells in culture have been reported by others to exhibit high levels of extracellular proteolytic (fibrinolytic) activity due to plasminogen activation, compared to low levels from nontransformed cells. Enhanced fibrinolysis was accordingly proposed to be a reliable and general enzymatic change associated with cell transformation.In the present study, ten different types of serially cultured, growing cells were examined for their extracellular fibrinolytic activity. The level of the fibrinolytic activity was found not to correlate with the transformed or nontransformed state of these cells. 相似文献
95.
Elaheh Jamasbi Steven Batinovic Robyn A. Sharples Marc-Antoine Sani Roy Michael Robins-Browne John D. Wade Frances Separovic Mohammed Akhter Hossain 《Amino acids》2014,46(12):2759-2766
Melittin (MLT) is a lytic peptide with a broad spectrum of activity against both eukaryotic and prokaryotic cells. To understand the role of proline and the thiol group of cysteine in the cytolytic activity of MLT, native MLT and cysteine-containing analogs were prepared using solid phase peptide synthesis. The antimicrobial and cytolytic activities of the monomeric and dimeric MLT peptides against different cells and model membranes were investigated. The results indicated that the proline residue was necessary for antimicrobial activity and cytotoxicity and its absence significantly reduced lysis of model membranes and hemolysis. Although lytic activity against model membranes decreased for the MLT dimer, hemolytic activity was increased. The native peptide and the MLT-P14C monomer were mainly unstructured in buffer while the dimer adopted a helical conformation. In the presence of neutral and negatively charged vesicles, the helical content of the three peptides was significantly increased. The lytic activity, therefore, is not correlated to the secondary structure of the peptides and, more particularly, on the propensity to adopt helical conformation. 相似文献
96.
Karen Luna-Ramírez Marc-Antoine Sani Jesus Silva-Sanchez Juana María Jiménez-Vargas Fernando Reyna-Flores Kenneth D. Winkel Christine E. Wright Lourival D. Possani Frances Separovic 《生物化学与生物物理学报:生物膜》2014
UyCT peptides are antimicrobial peptides isolated from the venom of the Australian scorpion. The activity of the UyCT peptides against Gram positive and Gram negative bacteria and red blood cells was determined. The membrane interactions of these peptides were evaluated by dye release (DR) of the fluorophore calcein from liposomes and isothermal titration calorimetry (ITC); and their secondary structure was determined by circular dichroism (CD). Three different lipid systems were used to mimic red blood cells, Escherichia coli and Staphylococcus aureus membranes. UyCT peptides exhibited broad spectrum antimicrobial activity with low MIC for S. aureus and multi-drug resistant Gram negative strains. Peptide combinations showed some synergy enhancing their potency but not hemolytic activity. The UyCT peptides adopted a helical structure in lipid environments and DR results confirmed that the mechanism of action is by disrupting the membrane. ITC data indicated that UyCT peptides preferred prokaryotic rather than eukaryotic membranes. The overall results suggest that UyCT peptides could be pharmaceutical leads for the treatment of Gram negative multiresistant bacterial infections, especially against Acinetobacter baumanni, and candidates for peptidomimetics to enhance their potency and minimize hemolysis. This article is part of a Special Issue entitled: Interfacially Active Peptides and Proteins. Guest Editors: William C. Wimley and Kalina Hristova. 相似文献
97.
Franco Andreone Luca Bartolozzi Giovanni Boano Ferdinando Boero Marco?A. Bologna Mauro Bon Nicola Bressi Massimo Capula Achille Casale Maurizio Casiraghi Giorgio Chiozzi Massimo Delfino Giuliano Doria Antonio Durante Marco Ferrari Spartaco Gippoliti Michele Lanzinger Leonardo Latella Nicola Maio Carla Marangoni Stefano Mazzotti Alessandro Minelli Giuseppe Muscio Paola Nicolosi Telmo Pievani Edoardo Razzetti Giorgio Sabella Marco Valle Vincenzo Vomero Alberto Zilli 《ZooKeys》2014,(456):139-146
The Italian natural history museums are facing a critical situation, due to the progressive loss of scientific relevance, decreasing economic investments, and scarcity of personnel. This is extremely alarming, especially for ensuring the long-term preservation of the precious collections they host. Moreover, a commitment in fieldwork to increase scientific collections and concurrent taxonomic research are rarely considered priorities, while most of the activities are addressed to public events with political payoffs, such as exhibits, didactic meetings, expositions, and talks. This is possibly due to the absence of a national museum that would have better steered research activities and overall concepts for collection management. We here propose that Italian natural history museums collaborate to instate a “metamuseum”, by establishing a reciprocal interaction network aimed at sharing budgetary and technical resources, which would assure better coordination of common long-term goals and scientific activities. 相似文献
98.
Isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the Homestake gold mine,Lead, South Dakota,USA 总被引:3,自引:0,他引:3
Gurdeep Rastogi Geetha L. Muppidi Raghu N. Gurram Akash Adhikari Kenneth M. Bischoff Stephen R. Hughes William A. Apel Sookie S. Bang David J. Dixon Rajesh K. Sani 《Journal of industrial microbiology & biotechnology》2009,36(4):585-598
The present study investigated the cultivable mesophilic (37°C) and thermophilic (60°C) cellulose-degrading bacterial diversity
in a weathered soil-like sample collected from the deep subsurface (1.5 km depth) of the Homestake gold mine in Lead, South
Dakota, USA. Chemical characterization of the sample by X-ray fluorescence spectroscopy revealed a high amount of toxic heavy
metals such as Cu, Cr, Pb, Ni, and Zn. Molecular community structures were determined by phylogenetic analysis of 16S rRNA
gene sequences retrieved from enrichment cultures growing in presence of microcrystalline cellulose as the sole source of
carbon. All phylotypes retrieved from enrichment cultures were affiliated to Firmicutes. Cellulose-degrading mesophilic and thermophilic pure cultures belonging to the genera Brevibacillus, Paenibacillus, Bacillus, and Geobacillus were isolated from enrichment cultures, and selected cultures were studied for enzyme activities. For a mesophilic isolate
(DUSELG12), the optimum pH and temperature for carboxymethyl cellulase (CMCase) were 5.5 and 55°C, while for a thermophilic
isolate (DUSELR7) they were 5.0 and 75°C, respectively. Furthermore, DUSELG12 retained about 40% CMCase activity after incubation
at 60°C for 8 h. Most remarkably, thermophilic isolate, DUSELR7 retained 26% CMCase activity at 60°C up to a period of 300 h.
Overall, the present work revealed the presence of different cellulose-degrading bacterial lineages in the unique deep subsurface
environment of the mine. The results also have strong implications for biological conversion of cellulosic agricultural and
forestry wastes to commodity chemicals including sugars. 相似文献
99.
Gurdeep Rastogi Shariff Osman Ravi Kukkadapu Mark Engelhard Parag A. Vaishampayan Gary L. Andersen Rajesh K. Sani 《Microbial ecology》2010,60(3):539-550
A microbial census on deep biosphere (1.34 km depth) microbial communities was performed in two soil samples collected from the Ross and number 6 Winze sites of the former Homestake gold mine, Lead, South Dakota using high-density 16S microarrays (PhyloChip). Soil mineralogical characterization was carried out using X-ray diffraction, X-ray photoelectron, and Mössbauer spectroscopic techniques which demonstrated silicates and iron minerals (phyllosilicates and clays) in both samples. Microarray data revealed extensive bacterial diversity in soils and detected the largest number of taxa in Proteobacteria phylum followed by Firmicutes and Actinobacteria. The archael communities in the deep gold mine environments were less diverse and belonged to phyla Euryarchaeota and Crenarchaeota. Both the samples showed remarkable similarities in microbial communities (1,360 common OTUs) despite distinct geochemical characteristics. Fifty-seven phylotypes could not be classified even at phylum level representing a hitherto unidentified diversity in deep biosphere. PhyloChip data also suggested considerable metabolic diversity by capturing several physiological groups such as sulfur-oxidizer, ammonia-oxidizers, iron-oxidizers, methane-oxidizers, and sulfate-reducers in both samples. High-density microarrays revealed the greatest prokaryotic diversity ever reported from deep subsurface habitat of gold mines. 相似文献
100.
Lucie Khemtmourian Marc‐Antoine Sani Katell Bathany Gerhard Grbner Erick J. Dufourc 《Journal of peptide science》2006,12(1):58-64
Solid phase synthesis of BH4, the 26 amino-acid domain (6RTGYDNREIVMKYIHYKLSQRGYEWD31) of the anti-apoptotic Bcl-2 protein has been accomplished using Fmoc chemistry. The use of peculiar cleavage conditions provided high yields after purification such that tens to hundreds of mg could be obtained. A 15N-labelled version of the peptide could also be synthesized for NMR studies in membranes. The peptide purity was not lower than 98% as controlled by UV and MALDI-TOF mass spectrometry. The secondary structure was determined in water, trifluoroethanol (TFE) and in lipid membrane using UV circular dichroism. The peptide shows dominant beta-sheeted structures in water that convert progressively into alpha-helical features upon addition of TFE or membrane. The amphipathic character of the helix suggests that the peptide might have a structure akin to those of antimicrobial peptides upon interaction with membranes. 相似文献