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121.
Isolation and protein pattern of eye lens fiber junctions 总被引:13,自引:0,他引:13
122.
The X Chromosomes of Mammals: Karyological Homology as Revealed by Banding Techniques 总被引:2,自引:0,他引:2
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A comparison of the Giemsa-banding patterns of the X chromosomes in various mammalian species including man indicates that two major bands (A and B), which are resistant to trypsin and urea-treatments, are always present irrespective of the gross morphology of the X chromosomes. This is true in all mammalian species with the "original or standard type" X chromosomes (5-6% of the haploid genome) thus far analyzed. In the unusually large-sized X chromosomes the extra chromosomal material may be due either to the addition of genetically inert constitutive heterochromatin or to an X-autosome translocation. In these X chromosomes two major bands are present in the actual X-chromosome segment. Our data on C and G band patterns also support Ohno's hypothesis that the mammalian X chromosome is extremely conservative in its genetic content, in spite of its cytogenetic variability. 相似文献
123.
D. Gupta I. Kovács 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1974,45(2):64-71
Summary Hybrids obtained from two series of diallel crosses made between six o
2 converted inbred lines on the one hand and their normal analogues on the other were compared for twenty-five characters including yield and several of the yield components, including the parents. Observations on simple inter-character correlation coefficients presented here have shown that the majority of the correlations at the o
2 level are of the same order as at the normal level. A number of correlations of the o
2 type are inferior to those of their normal analogues, whereas a few are favoured by the o
2 gene including the correlation of grain yield with kernels per row. A measure of heterosis for each hybrid over its mid-parent also demonstrated that the o
2 types show poorer heterosis in more cases than do their normal counterparts. Still, in nearly 40 percent of the cases the o
2 hybrids were found to be more heterotic than their normal analogues, particularly for the various maturity characters and several of the yield components. Thus, the possibility of improvement exists in breeding maize with the opaque-2 gene. 相似文献
124.
The 0.5M KCl wash of rabbit reticulocyte ribosomes (I fraction) catalyzes the deacylation of Met-tRNAfMet. Upon DEAE-cellulose column chromatography, the deacylase activity elutes with the 0.1M KCl wash of the column (f1) and is well-resolved from the peptide chain initiation factors (1–3). The deacylase activity is specific for Met-tRNAfMet (retic., ). Other aminoacyl tRNAs tested including fMet-tRNAfMet (retic., ), Phe-tRNA (), Val-tRNA (retic.), and Arg-tRNA (retic.) are completely resistant to the action of the deacylase. In the presence of the peptide chain initiation factor (IF1) and GTP, retic. Met-tRNAfMet forms the initiation complex Met-tRNAfMet:IF1:GTP (2), and in this ternary complex Met-tRNAfMet is not degraded by the deacylase. Met-tRNAfMet binds to IF1 independent of GTP, and in this complex, this Met-tRNAfMet is degraded by the deacylase.Prior incubation of f1 with Met-tRNAfMet (retic.) strongly inhibited protein synthesis initiation, presumably due to deacylation of the initiator tRNA. This inhibition by f1 was completely prevented when Met-tRNAfMet (retic.) was pre-incubated with peptide chain initiation factors. 相似文献
125.
S L Gupta M L Sopori P Lengyel 《Biochemical and biophysical research communications》1973,54(2):777-783
Protein synthesis directed by exogenous (viral or cellular) messengers is impaired, but endogenous protein synthesis is not affected in an extract of interferon-treated Ehrlich ascites tumor cells (INT-extract). Protein synthesis directed by exogenous messengers is also impaired in a mixture of an INT-extract with an extract from control cells. This reveals that the impairment is due to one or more inhibitors in the INT-extract. The nondialyzability of the inhibitor(s) is probably an indication of large molecular size. In a not incubated INT-extract much of the inhibitory activity is in the high speed sediment fraction i.e., is presumably bound directly or indirectly to ribosomes. During incubation of the extract most of the inhibitory activity is released into the high speed supernatant fraction. The dose-response curve shows that in our conditions the translation of cellular messengers (from mouse L cells) is as sensitive to impairment by the inhibitor(s) as that of viral messengers (from reovirus or from encephalomyocarditis virus). 相似文献
126.
Mass spectra of nucleoside components of tRNA 总被引:2,自引:0,他引:2
127.
128.
Attempts were made to transform two strains ofAzotobacter, viz.A. chroococcum andA. vinelandii with the DNA ofRhizobium leguminosarum, R. japonicum, R. meliloti andR. trifoli. Resistance to streptomycin or crystal violet was used as marker for screening purposes. The frequency of transformation varied between 0.05 to 0.95 percent. The transformants also acquired a few other characters of the donor strains. 相似文献
129.
Nocardiosis: A review 总被引:2,自引:0,他引:2