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41.
Sciutto E Fragoso G Fleury A Laclette JP Sotelo J Aluja A Vargas L Larralde C 《Microbes and infection / Institut Pasteur》2000,2(15):1875-1890
This article reviews current knowledge on human and porcine cysticercosis caused by Taenia solium. It highlights the conditions favorable for its prevalence and transmission, as well as current trends in research on its natural history, epidemiology, immunopathology, diagnosis, treatment and prevention. Our opinions on the most urgent needs for further research are also presented. 相似文献
42.
Diversity of terrestrial gastropod mollusks in the Pacific region of Nicaragua, and their habitat preferences. Landsnail communities of the Nicaraguan Pacific Slope were studied. The study area was subdivided into 21 quadrats (ca. 40 x 40 km). A high taxonomic richness was found: 79 species, 43 genera and 23 families. Species richness (S) per quadrat ranged from 17 through 50. The biogeographic index presented higher values on quadrats 5 (3.04), 6 (3.03), 8 (2.96) and 11(2.96). Quadrat 11 had the highest species richness (S=50), and one of the highest biogeographic index values (IB=2.96). Gastropod mollusk communities are favored by sites with an illumination of filtered sun, associated to riparian forests and with a susbtratum of wet soils and leaf litter. 相似文献
43.
Summary The morphogenesis of chromosomes at early prophase of spermatocytes I is studied in three ortoptherian species: Grillus argentinus (Grillidae), Laplatacris dispar (Acrididae) and Blaptica dubia (Blaptidae).The first chromosome component appearing at the beginning of meiosis is a thread (single elementary thread; S.E.T.) of low electron density measuring about 700 Å to 0.1 width. A group having the same width and integrated by three helically twisted ribbon-like components develops from S.E.T. and is called primary tripartite group, P.T.G. The three components are at first of the same width (about 200 Å) but the lateral arms progressively increase in thickness and in that way the group becomes coated by a layer of dense microfibrils supposed to be chromatin.Late stages of prophase were not thoroughly investigated in this study, but many evidences were however found helping to identify synaptene stage. In accordance with these evidences each homologous chromosome is integrated by an axial component (tripartite groups) coated by chromatin.The medial component of tripartite groups of Blaptica dubia is double and also shows multistranded regions which are called puffy regions.A comparative optical and electron microscope study was made in order to better understand the above described process. This study includes the comparison of the thickness of chromosomes as measured in light and electron micrographs.On the other hand, rubber models were made to illustrate the same process and pohotographs of these models are exhibited in the text.The nuclear structure of early spermatids of the same species is also studied in this paper. It was found that Blaptica and Grillus early spermatids nuclei contain groups similar to those found at the beginning of prophase of spermatocyte I, with the only difference that in many cases composite groups, formed by fusion of the lateral arms of two or more than two single groups, were found.The nuclear structure of late spermatids was also considered in this paper. Notwithstanding the study only aimed to point out that the pattern of organization of the spermatozoon chromatin greatly differs in the species examined. 相似文献
44.
Summary The radioautographic technique has been used to study the axonal migration of catecholamines in sympathetic fibres of the sciatic nerve of rats after ligature. A first series of experiments aimed at ascertaining the capacity of the proximal portion of adrenergic fibres to take up and store exogenous tritiated catecholamines (3H-DOPA; 3H-DA and 3H-NA) 3 to 22 hours after ligation. The results are qualitatively similar in rats pretreated and non-pretreated with IMAO, but the intensity of the radioautographic reaction is lower in the latter cases. Most of the labeled axons are filled mainly with vesicular and tubular profiles of endoplasmic reticular origin, large dense bodies (probably lysosomes) and/or mitochondria. The silver grains are generally superimposed on the vesicular and/or the tubular profiles, but in some cases on the large dense bodies, suggesting that exogenous catecholamines can be stored in lysosomes. The question whether SGV specifically store catecholamines also in the modified adrenergic fibres has been investigated in KMnO4 fixed material. These results show that beside a large number of fibres in which there is a strict correlation between labeling and SGV, some fibres containing SGV do not retain the 3H-NA. Conversely some fibres which contain mainly agranular vesicles display radioautographic reaction. Therefore, in case of ligated fibres, SGV cannot be considered the specific organelles for storage of catecholamines.The axonal migration of labeled catecholamines has been studied in animals pretreated with IMAO. A moderate, but selective, labeling is present in the proximal portion of sciatic fibres of rats in which administration of labeled catecholamine preceeded of 2 hours the ligature and this was performed 22 hours before fixation.From these combined types of experiments, it is concluded that despite the presence of all the structures necessary for the storage of a high amount of catecholamines in the modified adrenergic fibres, only a small fraction of catecholamines accumulated above the ligature has been transported by axonal migration. Therefore, the axonal migration of catecholamines appears as an epiphenomenon related to the distal migration of enzymatic and storage proteins from the perikaryon.Part of this work was presented to the 84th Annual Session of American Association of Anatomists (Sotelo and Taxi, 1971).Some of the electron microscopic observations were made at the Unité 106 I.N.S.E.R.M. 相似文献
45.
Miquet JG Sotelo AI Bartke A Turyn D 《American journal of physiology. Endocrinology and metabolism》2005,289(4):E600-E607
Chronic exposure to growth hormone (GH) was related to the desensitization of the JAK2/STAT5 signaling pathway in liver, as demonstrated in cells, female rats, and transgenic mice overexpressing GH. The cytokine-induced suppressor (CIS) is considered a major mediator of this desensitization. Pregnancy is accompanied by an increment in GH circulating levels, which were reported to be associated with hepatic GH resistance, although the molecular mechanisms involved in this resistance are not clearly elucidated. We thus evaluated the JAK2/STAT5b signaling pathway and its regulation by the suppressors of cytokine signaling (SOCS)/CIS family and the JAK2-interacting protein SH2-Bbeta in pregnant mouse liver, a model with physiological prolonged exposure to high GH levels. Basal tyrosyl phosphorylation levels of JAK2 and STAT5b in pregnant mice were similar to values obtained for virgin animals, in spite of the important increment of GH they exhibit. Moreover, these signaling mediators were not phosphorylated upon GH stimulation in pregnant mice. A 3.3-fold increase of CIS protein content was found for pregnant mice, whereas the abundance of the other SOCS proteins analyzed and SH2-Bbeta did not significantly change compared with virgin animals. The desensitization of the JAK2/STAT5b GH signaling pathway observed in pregnant mice would then be mainly related to increased CIS levels rather than to the other regulatory proteins examined. 相似文献
46.
Sanford L. Palay Constantino Sotelo Alan Peters Paula M. Orkand 《The Journal of cell biology》1968,38(1):193-201
Axon hillocks and initial segments have been recognized and studied in electron micrographs of a wide variety of neurons. In all multipolar neurons the fine structure of the initial segment has the same pattern, whether or not the axon is ensheathed in myelin. The internal structure of the initial segment is characterized by three special features: (a) a dense layer of finely granular material undercoating the plasma membrane, (b) scattered clusters of ribosomes, and (c) fascicles of microtubules. A similar undercoating occurs beneath the plasma membrane of myelinated axons at nodes of Ranvier. The ribosomes are not organized into Nissl bodies and are too sparsely distributed to produce basophilia. They vanish at the end of the initial segment. Fascicles of microtubules occur only in the axon hillock and initial segment and nowhere else in the neuron. Therefore, they are the principal identifying mark. Some speculations are presented on the relation between these special structural features and the special function of the initial segment. 相似文献
47.
Electron microscope study on the development of ciliary components of the neural epithelium of the chick embryo 总被引:1,自引:0,他引:1
Summary Ciliary development was studied in the cells of the neural canal of chick embryos incubated from 60 hours to 7 days.It was found that centrioles move after the last mitosis to the cell periphery where one of them enters into contact (terminal contact) with the cell membrane; the other centriole remains close by, its axis aligned along the axis of the former.The cell membrane was seen afterwards bulging at the contact point, and the content of the ciliary bud thus formed is only constituted at the beginning of a varied number of vesicles of about 140 Å diameter.The ciliary bud becomes elongated shortly after filaments start becoming organized in the bud matrix.Roughly coinciding with the initiation of filament organization the centrioles move inward and the cilium becomes deeply invaginated in the cell. At the end of ciliary growth the centriole moves again toward the surface and the cilium emerges in the neural canal lumen. 相似文献
48.
To study the adaptative capabilities of the retinotectal system in birds, the primordium of one optic tectum from 12-somite embryos of Japanese quail was transplanted either homotopically, to replace the ablated same primordium, or heterotopically, to replace the ablated dorsal diencephalon in White Leghorn chick embryos of the same stage. The quail nucleolar marker was used to recognize the transplants. The cytoarchitecture of the tecta and the retinal projections from the eye contralateral to the graft were studied on the 17th or 18th day of incubation in the chimeric embryos by autoradiographic or horseradish peroxidase tracing methods. Morphometric analysis was applied to evaluate the percentage of the tectal surface receiving optic projections. It was observed that: (i) quail mesencephalic alar plate can develop a fully laminated optic tectum even when transplanted heterotopically; (ii) retinal ganglion cells from the chick not only recognize the tectal neurons of the quail as their specific targets in homotopic grafts, but the optic fibers deviate to innervate the heterotopically grafted tectum; (iii) in the presence of a graft, the chick retina is unable to innervate a tectal surface of similar or larger size than that of the control tectum; (iv) tectal regions devoid of optic projections, whether formed by donor or by host cells, always present an atrophic lamination; (v) the diencephalic supernumerary optic tectum competes with and prevails over the host tectum as a target for optic fiber terminals. 相似文献
49.
T. Kuramoto C. Sotelo N. Yokoi T. Serikawa E. Goñalons Sintes J. Cantó Martorell J. -L. Guénet 《Mammalian genome》1996,7(12):890-894
A recessive mutation exhibiting severe myelin breakdown, mainly at the level of the lumbar segments of the spinal cord and
without any associated inflammation, was discovered in a partially inbred rat colony. Analysis of the segregation patterns
of a set of polymorphic microsatellite markers in two inter-strain crosses allowed the mapping of this autosomal recessive
mutation to rat Chromosome (Chr) 17, very close to the prolactin (Prl) locus, in a region homologous to human Chr 6p21.2-22.3 and mouse Chr 13. The pathology of the demyelination process and
the chromosomal localization indicate that this mutation has no known equivalent in either mouse or human.
Received: 21 March 1996 / Accepted: 22 July 1996 相似文献
50.
Carla Díaz-Tielas Tamara Sotelo Elisa Graña Manuel J. Reigosa Adela M. Sánchez-Moreiras 《Journal of Plant Growth Regulation》2014,33(2):181-194
Chalcones are flavonoid precursors that recently have been found to disrupt mitochondrial function and induce apoptosis in Arabidopsis radicles. However, whether they are potentially useful for weed control as selective herbicides depends on whether they are phytotoxic for important weeds and are not toxic for crops. This work determines the phytotoxicity of chalcone for the germination and early development of a variety of crop species and associated weeds. Also, the phytotoxic potential was investigated in chalcone-watered or sprayed adult plants of Arabidopsis, a common model in phytotoxicity assays. Chalcone was detrimental mainly to the germination of Plantago lanceolata and Lactuca sativa and to the early root growth of Amaranthus retroflexus, Echinochloa crus-galli, and P. lanceolata, and likewise detrimental to the development of adult Arabidopsis. The morphology and physiology of adult Arabidopsis plants watered or sprayed with chalcone confirmed that this metabolite is also phytotoxic for adult plants and showed that the mechanism of its action on plant metabolism depends on whether it is administered by spraying or watering. These results support the role of chalcone as a plant growth regulator and its potential use in weed management in the field. 相似文献